Welcome Each of
You to My
Molecular Biology
Class
Molecular Biology of the Gene,
5/E --- Watson et al,(2004)
Part I,Chemistry and Genetics
Part II,Maintenance of the Genome
Part III,Expression of the Genome
Part IV,Regulation
Part V,Methods
3/29/05
Part II,Maintenance of the Genome
Dedicated to the structure of
DNA and the processes that
propagate,maintain and alter it
from one cell generation to the
next
Ch 6,The structures of DNA and RNA
Ch 7,Chromosomes,chromatins and
the nucleosome
Ch 8,The replication of DNA
Ch 9,The mutability and repair of
DNA
Ch 10,Homologous recombination at
the molecular level
Ch 11,Site-specific recombination
and transposition of DNA
3/29/05
CHAPTER 10 Homologous
recombination at the
molecular level
?Molecular Biology Course
? All DNA is recombinant DNA,
? Genetic exchange works constantly
to blend and rearrange
chromosomes,most obviously
during meiosis,when homologous
chromosomes pair prior to the first
nuclear division,
? During this pairing,genetic
exchange between the
chromosomes occurs,This
exchange,classically termed
crossing over,is one of the results
of homologous recombination,
OUTLINE
1,Models for Homologous
Recombination (two models)**
2,Homologous Recombination Protein
Machines (RecBCD pathway)**
3,Homologous Recombination in
Eukaryotes (meiosis recombination,
proteins)
4,Mating-Type Switching (a gene
conversion event)
5,Genetic Consequences of the
Mechanism of Homologous
Recombination (exchange or
conversion)
重点和难点
3/29/05
Topics 1 & 2 are the keys to
understand homologous
recombination and other
topics,
Completely understand two
animations on your CD
CHAPTER 10 Homologous recombination at the molecular level
Review your textbook
Homework
CHAPTER 9 The mutability and repair of DNA