Is there any similarity between human and model
organisms?
Human development
Drosophila embryos
Using functional conservation to clone human
genes
Using mutants to analyze the pathway or
functions of genes
Mutants have to be generated to analyze the pathway
or functions of genes
Saturation screening
Overexpression
Suppressor mutations
Synthetic lethals
Nonallelic noncomplementation
Saturation
screening
may not
recover all
genes
Overexpression
Suppressor mutations
Synthetic lethals
Nonallelic noncomplementation
Developmental genetics-YEAST model
Three kinds of
plasmids used
by yeast
geneticists
Gene disruption in yeast by homologous
recombination
Cell type
differentiation in
yeast
Homothallic yeast can switch mating type
Yeast mating is mediated by pheromones
Mating signal induces physiological changes
Mice genetics
Early
mammalian
embryos are
highly
malleable
Generating
transgenic
mice
Using transgenic technology to prove SRY as
maleness development gene
Short-tail
phenotype
caused by T
locus mutation
is due to
pme75 gene
deletion
Targeting
mutagenesis in
mice