Welcome Each of
You to My
Molecular Biology
Class
Molecular Biology of the Gene,
5/E --- Watson et al,(2004)
Part I,Chemistry and Genetics
Part II,Maintenance of the Genome
Part III,Expression of the Genome
Part IV,Regulation
Part V,Methods
3/01/05
Question,Why Watson et al,
want to tell us some stories
about genetics and chemistry
before we start the molecular
biology?
3/01/05
Chemistry and Genetics
Ch 1,The Mendelian View of the World
Ch 2,Nucleic Acids convey genetic
information
Ch 3,The importance of weak
chemical interactions
Ch 4,The importance of high-energy
bonds
Ch 5,Weak and strong bonds
determine macromolecular structure
3/01/05
Chapter 1,The Mendelian
View of the World
To explain how the genetic traits
are transferred from the parents to
the offsprings
Two + one person tell us
one big story
3/01/05
Two important lessons to
learn from Mendel’s discovery
1,The attitude to science:
interested in discovering the
wonders of the nature (laws,
mechanisms)
2,The principle to conduct science:
using simple models to
understand the complicate or
unknown story
3/01/05
Discussion
Why Mendelianism and Darwinism
conflicted initially,how the
conflict was resolved? (P15-16)
3/01/05
Chapter 2,Nucleic Acids
convey genetic information
The stories/efforts led to the
fundamental knowledge of life,
which open a new era of modern
biology
3/04/05
Ch 2 Nucleic Acids convey genetic information
Avery’s Bombshell (1944),
DNA can carry genetic specificity
3/04/05
The Double Helix (1953)
The genetic information within DNA is conveyed by
the sequence of its four nucleotide building blocks
Establishing the direction of protein synthesis
The Central Dogma (1956)
The Era of Genomics (2000)
Avery’s Bombshell (1944),
DNA can carry genetic specificity
A story written for 26 years
Thinking 1,what do you learn
from the efforts of George W,
Beadle and Edward Tatum (1940s),
Frederick Griffith (1928),Osward T,
Avery (1940s)
1928 by Frederick
Griffith
1944 by Osward T,Avery
Thinking 2,Why Avery made
more recognizable contribution to
the field than did Griffith?
The Double Helix (1953)
The foundation of molecular biology
Francis H,
Crick
James D,
Watson
Thinking 3,what led to the success
of Watson and Crick?
The Central Dogma (1953-1956)
Setting the platform for molecular
biology only takes 0.5 year or 3.5
years
Exciting days of biologists
DNA RNA Protein
Replication
Transcription
Translation
The revised central dogma
Gene
Expression
The purpose of Molecular Biology
of the genes is to provide a firm
foundation for understanding how
DNA functions as the template for
biological complexity,
3/04/05
Ch 2 Nucleic Acids convey genetic information
Remember,this book is living in the
age of the Central Dogma in
understanding life,post-genomic era
will certainly tell us a more exciting
and different story of life
Part 2,Maintenance of the Genome,
describes the structure of the genetic
material and its faithful duplication.
Part 3,Expression of the Genome,
shows how the genetic instructions
contained in DNA is converted into
proteins
Part 4,Regulation,describes
strategies for differential gene activity
that are used to generate complexity
within the organisms and diversity
among organisms 3/04/05
Part 5,Methods,describes various
laboratory techniques,bioinformatics
approaches,and model systems that
are commonly used to investigate
biological problem.
3/04/05
Chapter 3,The importance of
weak chemical interactions
Weak bonds indeed are vital
for life,partly because they
can form and break under the
physiological conditions
present with cells,
(Dynamics is important)
3/04/05
Pages 45-53 are important to
read
3/04/05
List the reasons making weak
bond vital for life
Chapter 5,Weak and strong
bonds determine
macromolecular structure
3/04/05
Ch 5,Weak and strong bonds determine
macromolecular structure
?Higher-order structures are determined
by intra- and intermolecular interactions
?The specific conformation of a protein
results from its pattern of hydrogen bonds
?Most proteins are modular,containing
two or three domains
?Weak bonds correctly position proteins
along DNA and RNA molecules
?Allostery,Regulation of a protein’s
function by changing its shape
3/04/05
You to My
Molecular Biology
Class
Molecular Biology of the Gene,
5/E --- Watson et al,(2004)
Part I,Chemistry and Genetics
Part II,Maintenance of the Genome
Part III,Expression of the Genome
Part IV,Regulation
Part V,Methods
3/01/05
Question,Why Watson et al,
want to tell us some stories
about genetics and chemistry
before we start the molecular
biology?
3/01/05
Chemistry and Genetics
Ch 1,The Mendelian View of the World
Ch 2,Nucleic Acids convey genetic
information
Ch 3,The importance of weak
chemical interactions
Ch 4,The importance of high-energy
bonds
Ch 5,Weak and strong bonds
determine macromolecular structure
3/01/05
Chapter 1,The Mendelian
View of the World
To explain how the genetic traits
are transferred from the parents to
the offsprings
Two + one person tell us
one big story
3/01/05
Two important lessons to
learn from Mendel’s discovery
1,The attitude to science:
interested in discovering the
wonders of the nature (laws,
mechanisms)
2,The principle to conduct science:
using simple models to
understand the complicate or
unknown story
3/01/05
Discussion
Why Mendelianism and Darwinism
conflicted initially,how the
conflict was resolved? (P15-16)
3/01/05
Chapter 2,Nucleic Acids
convey genetic information
The stories/efforts led to the
fundamental knowledge of life,
which open a new era of modern
biology
3/04/05
Ch 2 Nucleic Acids convey genetic information
Avery’s Bombshell (1944),
DNA can carry genetic specificity
3/04/05
The Double Helix (1953)
The genetic information within DNA is conveyed by
the sequence of its four nucleotide building blocks
Establishing the direction of protein synthesis
The Central Dogma (1956)
The Era of Genomics (2000)
Avery’s Bombshell (1944),
DNA can carry genetic specificity
A story written for 26 years
Thinking 1,what do you learn
from the efforts of George W,
Beadle and Edward Tatum (1940s),
Frederick Griffith (1928),Osward T,
Avery (1940s)
1928 by Frederick
Griffith
1944 by Osward T,Avery
Thinking 2,Why Avery made
more recognizable contribution to
the field than did Griffith?
The Double Helix (1953)
The foundation of molecular biology
Francis H,
Crick
James D,
Watson
Thinking 3,what led to the success
of Watson and Crick?
The Central Dogma (1953-1956)
Setting the platform for molecular
biology only takes 0.5 year or 3.5
years
Exciting days of biologists
DNA RNA Protein
Replication
Transcription
Translation
The revised central dogma
Gene
Expression
The purpose of Molecular Biology
of the genes is to provide a firm
foundation for understanding how
DNA functions as the template for
biological complexity,
3/04/05
Ch 2 Nucleic Acids convey genetic information
Remember,this book is living in the
age of the Central Dogma in
understanding life,post-genomic era
will certainly tell us a more exciting
and different story of life
Part 2,Maintenance of the Genome,
describes the structure of the genetic
material and its faithful duplication.
Part 3,Expression of the Genome,
shows how the genetic instructions
contained in DNA is converted into
proteins
Part 4,Regulation,describes
strategies for differential gene activity
that are used to generate complexity
within the organisms and diversity
among organisms 3/04/05
Part 5,Methods,describes various
laboratory techniques,bioinformatics
approaches,and model systems that
are commonly used to investigate
biological problem.
3/04/05
Chapter 3,The importance of
weak chemical interactions
Weak bonds indeed are vital
for life,partly because they
can form and break under the
physiological conditions
present with cells,
(Dynamics is important)
3/04/05
Pages 45-53 are important to
read
3/04/05
List the reasons making weak
bond vital for life
Chapter 5,Weak and strong
bonds determine
macromolecular structure
3/04/05
Ch 5,Weak and strong bonds determine
macromolecular structure
?Higher-order structures are determined
by intra- and intermolecular interactions
?The specific conformation of a protein
results from its pattern of hydrogen bonds
?Most proteins are modular,containing
two or three domains
?Weak bonds correctly position proteins
along DNA and RNA molecules
?Allostery,Regulation of a protein’s
function by changing its shape
3/04/05
