Study on Fertility Characteristic and Development
on New System of Male Sterile Lines
of Nian竹pe in Wheat
MA,Postgraduate,Na Niu
Tutor,Prof,Gaisheng Zhang
(Northwest Sci.&Tech,University of Agriculture and Forestry,
Yangting,Shaanxi,712100)
Abstract
The CMS is one of the main ways to make use of wheat heterosis,In order to probe into
and open out the fertility characteristics of male sterile lines of Nian type in wheat deeply,to
utilize characteristics as fertility gene single of it,and to establish a undivided convertible
breeding system of it,the principle materials,such as sterile gene carriers different resources,
a series of non-IBLYIRS male sterile lines of different cytoplasms and identical nucleus,
identical cytoplasms and diversified nucleus and different cytoplasms and nucleus,are
utilized,and four contents are studied in this research,1,to screen the different fertility gene
carriers and compare fertility characteristic of male sterile lines of Nian type in wheat; 2,to
study inheritance on restoring performance of it; 3,to analyze the genetic mechanism of male
and female gametes transferring in sterile lines and Ft; 4,to establish the fast convertible
breeding system of it,The results are as follows.
1,By screening the non-IBL/I RS male sterile carriers of Nian type from different
resources in wheat and combining with cytology identification,we can find that SP4,Tmacha,
90-110,224 are belong to the types of non- I BL/IRS male sterility,They manifest complete
male sterility,and the sterility are very stable,After non- IBL/IRS male sterile carriers SP4,
Tmacha,90-110,224，led into the allocytoplasms with Ae.kotschyi,Ae.variabilis,
Ae.ventricosa and Ae.bicornis cytoplasms,The results of mensuration to their fertility
restoring performances show as follows,The restoring performances of sterile carriers with
Ae.kotschyi and Ae,ventricosa are quite different,The restoring degree of Alloplasms SP4 and
T.macha sterile carriers respectively is lower,The range of restoring degree is wider,The C,V
is higher,and F,plants of these two sterile types appear sterility separation in genotype of
special father or sterile cytoplasms,The restoring performances of Alloplasms 90-110 and
224 are fine,They don't appear sterility separation,The C.V is lower,Compared with SP4
and T macha，the difference of average seed set is best significant,So 90-110 and 224 are the
fine resources of maintaining lines,They have the vast prospects for making use of heterosis
in wheat.
2,The fertility restoring performance of the 4 male sterile lines of alloplasmic
non-IBLIIRS wheat with Aegilops koisc句4 Ae.varibifs,Ae,ventricosa and Ae,bicornis
cytoplasms are systematically investigated in this paper,The results are as follows,The 4
male sterile lines of alloplasmic non-1BL/IRS wheat tested are all belonged to the sterile type
of both good restoration and fine maintenance performance,When the 4 male sterile lines of
alloplasmic non-1BUIRS wheat,as female parents,are testcrossed with an identical male
parent or many different male parents,there is si娜ficant negative correlation between the
average seed set of F,and the C.V,of the seed set among the single plant,As for the 4 male
sterile lines of alloplasmic non- 1BL/1 RS wheat,their average seed set of Fi is influenced by
its alloplasmic cytoplasm to some extent,But there is not Significance of difference among
different sterile cytoplasms,Though the fertility carrier of the 4 male sterile lines of
alloplasmic non-1BL/IRS wheat is the same each other,the location of fertility loci in the
same linkage group,besides the male sterile type with Ae.kotschyi,Ae.varibilis cytoplasms is
identical,may be different respectively.The some minor enhance gene and inhibiting gene of
fertility,besides major fertility gene,are in the different karyotype of the male sterile lines and
the restorer lines of 4 alloplasmic non-1BUIRS wheat,The forms constituted and combined
of gene from different parent lines is important judgement index of the fertility restoring
degree of NIAN type
3,The manner of gametes transmission of non-IBUIRS male sterile lines with
Ae.kotschyi Ae.variabilis,Ae.ventricosa and Ae.bicornis cytoplasms in wheat and Fi is
analyzed in this paper,Obviously,non-IBUIRS male sterile lines of Nian type have a
characteristic of gametophyte sterility,In F2 groups,Most of combinations supplied appear
fertile,There is not fertility separation,And Ttest on hybridization Fi,F2 indicates that the
percent of average seed set is higher in F2 than Fl,These results prove once again that the
hybridization Fi of non-IBL/IRS male sterile lines with Ae.kotschyi,Ae.variabilis and
Ae.bicornis cytoplasms inherits in mode of gametophyte sterility.
4,The four non-IBUIRS male sterile lines of Nian type as mother parents cross with
Abbondanza 1B nullisomic,Chinese Spring nullitetrasomic IB0ID",Chinese Spring,When
the chromosome I B on Chinese Spring disomic exists,the four male sterile lines can be
restored,When the chromosome 1B on Abbondananza IB nullisomic and Chinese Spring
nullitetrasomic IB0ID" are deficient,they can not be restored,That shows thei sterile gene
single,and sterile gene lies on the chromosome 1B,this paves the way for establishing a set
of method system that fast creats Nian type non-1BL/IRS male sterile line in wheat,And
this supplies a new method for use strong predominant combination in many ways.
Key words,wheat,male sterile line,restoring performance,gamete transmission,
chromosome engineering
on New System of Male Sterile Lines
of Nian竹pe in Wheat
MA,Postgraduate,Na Niu
Tutor,Prof,Gaisheng Zhang
(Northwest Sci.&Tech,University of Agriculture and Forestry,
Yangting,Shaanxi,712100)
Abstract
The CMS is one of the main ways to make use of wheat heterosis,In order to probe into
and open out the fertility characteristics of male sterile lines of Nian type in wheat deeply,to
utilize characteristics as fertility gene single of it,and to establish a undivided convertible
breeding system of it,the principle materials,such as sterile gene carriers different resources,
a series of non-IBLYIRS male sterile lines of different cytoplasms and identical nucleus,
identical cytoplasms and diversified nucleus and different cytoplasms and nucleus,are
utilized,and four contents are studied in this research,1,to screen the different fertility gene
carriers and compare fertility characteristic of male sterile lines of Nian type in wheat; 2,to
study inheritance on restoring performance of it; 3,to analyze the genetic mechanism of male
and female gametes transferring in sterile lines and Ft; 4,to establish the fast convertible
breeding system of it,The results are as follows.
1,By screening the non-IBL/I RS male sterile carriers of Nian type from different
resources in wheat and combining with cytology identification,we can find that SP4,Tmacha,
90-110,224 are belong to the types of non- I BL/IRS male sterility,They manifest complete
male sterility,and the sterility are very stable,After non- IBL/IRS male sterile carriers SP4,
Tmacha,90-110,224，led into the allocytoplasms with Ae.kotschyi,Ae.variabilis,
Ae.ventricosa and Ae.bicornis cytoplasms,The results of mensuration to their fertility
restoring performances show as follows,The restoring performances of sterile carriers with
Ae.kotschyi and Ae,ventricosa are quite different,The restoring degree of Alloplasms SP4 and
T.macha sterile carriers respectively is lower,The range of restoring degree is wider,The C,V
is higher,and F,plants of these two sterile types appear sterility separation in genotype of
special father or sterile cytoplasms,The restoring performances of Alloplasms 90-110 and
224 are fine,They don't appear sterility separation,The C.V is lower,Compared with SP4
and T macha，the difference of average seed set is best significant,So 90-110 and 224 are the
fine resources of maintaining lines,They have the vast prospects for making use of heterosis
in wheat.
2,The fertility restoring performance of the 4 male sterile lines of alloplasmic
non-IBLIIRS wheat with Aegilops koisc句4 Ae.varibifs,Ae,ventricosa and Ae,bicornis
cytoplasms are systematically investigated in this paper,The results are as follows,The 4
male sterile lines of alloplasmic non-1BL/IRS wheat tested are all belonged to the sterile type
of both good restoration and fine maintenance performance,When the 4 male sterile lines of
alloplasmic non-1BUIRS wheat,as female parents,are testcrossed with an identical male
parent or many different male parents,there is si娜ficant negative correlation between the
average seed set of F,and the C.V,of the seed set among the single plant,As for the 4 male
sterile lines of alloplasmic non- 1BL/1 RS wheat,their average seed set of Fi is influenced by
its alloplasmic cytoplasm to some extent,But there is not Significance of difference among
different sterile cytoplasms,Though the fertility carrier of the 4 male sterile lines of
alloplasmic non-1BL/IRS wheat is the same each other,the location of fertility loci in the
same linkage group,besides the male sterile type with Ae.kotschyi,Ae.varibilis cytoplasms is
identical,may be different respectively.The some minor enhance gene and inhibiting gene of
fertility,besides major fertility gene,are in the different karyotype of the male sterile lines and
the restorer lines of 4 alloplasmic non-1BUIRS wheat,The forms constituted and combined
of gene from different parent lines is important judgement index of the fertility restoring
degree of NIAN type
3,The manner of gametes transmission of non-IBUIRS male sterile lines with
Ae.kotschyi Ae.variabilis,Ae.ventricosa and Ae.bicornis cytoplasms in wheat and Fi is
analyzed in this paper,Obviously,non-IBUIRS male sterile lines of Nian type have a
characteristic of gametophyte sterility,In F2 groups,Most of combinations supplied appear
fertile,There is not fertility separation,And Ttest on hybridization Fi,F2 indicates that the
percent of average seed set is higher in F2 than Fl,These results prove once again that the
hybridization Fi of non-IBL/IRS male sterile lines with Ae.kotschyi,Ae.variabilis and
Ae.bicornis cytoplasms inherits in mode of gametophyte sterility.
4,The four non-IBUIRS male sterile lines of Nian type as mother parents cross with
Abbondanza 1B nullisomic,Chinese Spring nullitetrasomic IB0ID",Chinese Spring,When
the chromosome I B on Chinese Spring disomic exists,the four male sterile lines can be
restored,When the chromosome 1B on Abbondananza IB nullisomic and Chinese Spring
nullitetrasomic IB0ID" are deficient,they can not be restored,That shows thei sterile gene
single,and sterile gene lies on the chromosome 1B,this paves the way for establishing a set
of method system that fast creats Nian type non-1BL/IRS male sterile line in wheat,And
this supplies a new method for use strong predominant combination in many ways.
Key words,wheat,male sterile line,restoring performance,gamete transmission,
chromosome engineering
