BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Lecture 4: Degradable Materials with Biological Recognition (part II) Last time: Biological recognition in vivo Engineering biological recognition of biomaterials: adhesion/migration peptides Today: Engineering biological recognition of biomaterials: enzymatic recognition and cytokine signaling Reading: S.E. Sakiyama-Elbert and J.A. Hubbell, ‘Functional Biomaterials: Design of Novel biomaterials,’ Annu. Rev. Mater. Sci. 31, 183-201 (2001) J.C. Schense et al., ‘Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension,’ Nat. Biotech. 18, 415-419 (2000) Supplementary Reading: ‘The Extracellular Matrix,’ pp. 1124-1150, Molecular Biology of the Cell, Lodish et al. Recognition of Biomaterials by Proteases: Engineering Enzyme-mediated degradation of polymers Basic concept: include peptide sequences in the polymer chain which are cleaved by enzymatic activity of serum proteins/cellular secreted products (active breakdown) [hydrolysis active but slow...] ? Amide bond -(NH)-(CO)- provides natural hydrolytic mechanism for degradation, but breaks down very slowly in physiological conditions Remodeling enzymes in vivo: 1) binding of target by enzyme 2) specific site cleavage ? What is enzymatic cleavage used for in vivo? 1 (Reading Ch.1 4 Voet and Voet “Enzymatic catalysis”) o Remodling ECM, migration of cells through matrix o Removing functional groups from signaling molecules (phosphorylation/dephosphorylation) ? Utility in the design of biomaterials: o Enzymatic cleavage can breakdown polymers more quickly than hydrolysis o Polymers can be designed to be surface-eroding easily (enzyme can’t access interior) o Degradation could be localized to tissue where enzyme is produced Lecture 4 – Biological Recognition pt. 2 1 of 9 BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Reminder of breakdown mechanisms: Used for biodegradable hydrogels Main mechanism exploited in solid polymers (Ratner, Biomaterials Science) (SLIDE) Cell source Cleavage of synthetic polymers by enzymes Enzyme Native function Acts on Degradation Mechanism Result Various bacteria lipases protease Polyesters, polyesteramides III Monomers or dimers Tritirachium album (mold) Proteinase K Protease Poly(lactide) III Monomers or dimers Mammalian cells esterases protease Poly(alkyl cyanoacrylates) polyesteramides 2 II Water-soluble polymers Mammalian cells Papain, pepsin proteases III Untested Mammalian cells α-chymotrypsin Serine protease Aromatic peptides in polyesteramides 3 (e.g. Ala, Val, Leu) III Untested Mammalian cells elastase protease Polyesteramides III untested null Pepsin: protease from papaya null Papain: main protease in gastric juice of stomach null Chymotrypsin: digestive enzyme null Mold and bacterial proteases not relevant in vivo, but make these polymers also of significant interest for environmentally-friendly packaging ? Of interest in the use of biodegradable materials in environmentally-friendly packaging, but not a concern for in vivo applications Lecture 4 – Biological Recognition pt. 2 2 of 9 BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 o Data comparing in vitro and in vivo degradation rates indicates that enzymatic cleavage of most synthetic polymers is negligible o Polyesteramides have been synthesized with enzymatic recognition: Enzymatic attack on polyesteramides (SLIDE) ? Breakdown by mechanism III ? Enzymatic breakdown of polymers is fast relative to simple hydrolysis: Polyesteramide breakdown by papain: ? What does papain do in vivo? Esterase action on poly(alkyl cyanoacrylates 4 ): C≡N C≡N -(CH 2 -C-) n - H 2 O -(CH 2 -C-) n - C=O C=O O O - Poly(akyl cyanoacrylates): R formation of poly(2-cyanoacrylic acid) _ _ _ _ _ _ _ Lecture 4 – Biological Recognition pt. 2 3 of 9 BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 ? Breakdown by mechanism II Mechanism: data on degradation of 250nm-diam. porous particles: (SLIDE) ? What does esterase do in vivo? Enzymatic activity in vivo on peptide sequences: 5,6 Cleavage Enzyme Functions in vivo Target amino acid sequences Plasminogen activator (urokinase or tissue-type plasminogen activator) / plasminogen → plasmin Degradation of fibrin matrices, angiogenesis, tumor progression; urokinase can bind to cell surface receptor on fibrinogen: Arg 104 -Asp 105 , Arg 110 -Val 111 , Lys 206 -Met207, Arg 42 -Ala 43 , Lys 130 - Glu 131 , Lys 84 -Ser 85 , Lys 87 -Met 88 Matrix metalloproteinases (soluble and cell-surface): e.g. Fibroblast Collagenase (MMP I) Facilitate cell migration Type I collagen: Gly 775 -Ile 776 In smaller peptides: Gly-Leu or Gly Ile bonds Elastase Elastin remodeling Poly(Ala) sequences REFS for MMP: (REF J. Biol. Chem. 256, 9511 (1981); J. Biol. Chem. 264, 393 (1989); J. Biol.l Chem. 266, 6747 (1991)) Note that proteases often have complementary protease inhibitors: e.g. plasminogen activators inhibited by plasminogen activator inhibitor type-1 (a serine protease inhibitor) Lecture 4 – Biological Recognition pt. 2 4 of 9 BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Examples of peptide sequences used to allow enzymatic cleavage in biomaterials (Work led by J. West and J.A. Hubbell 7-9 ) recognition by collagenase: -Ala-Pro-Gly-/-Leu- recognition by plasmin: -Val-Ala-/-Asn- recognition by elastase: -Ala-Ala-Ala-Ala-Ala- (polyalanine sequence) Example: poly(ethylene glycol) networks: acrylate-APGL-PEG-LGPA-acrylate (ON BOARD) Acrylate endgroups PEG collagenase sequence photopolymerization = = -APGL- -CH 2 CH 2 O- collagenase -APGL- collagenase peptides network structure formed by stitching together short strings of acrylate endgroups Lecture 4 – Biological Recognition pt. 2 5 of 9 BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 null Initially, swelling of network increases and wet weight goes up as first cross-links are broken, then as chains are freed and begin to diffuse out, weight goes down degradation rate of network depends on collagenase concentration (from Mann et al.): ? note that these are hydrogels which has a major impact on the degradation rate… ? degradation rate is controlled by enzyme concentration and is selective for the enzyme targeted Any examples tested in vivo? Recognition of Biomaterials by Cytokine Resceptors: Engineering growth and differentiation of cells on biomaterials via cytokine peptides 10-12 ? Growth factors small proteins (ca. 50 aa) ? Can be immobilized to polymer chains present at surfaces of biomaterials much like adhesion peptides and presented to receptors of cells ? Many growth factors signal by DIMERIZATION and autophosphorylation null Again, spatial distribution may be key in controlling signaling null Cytokines immobilized in close proximity may favor signaling (Lodish Fig. 20-32) Lecture 4 – Biological Recognition pt. 2 6 of 9 BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 ? INTERNALIZATION: used to down-regulate signals null Immobilized cytokines may show more potent signaling due to lack of this down-regulation pathway ? One of the first examples- Griffith lab work with epidermal growth factor immobilized on star polymers: Lecture 4 – Biological Recognition pt. 2 7 of 9 BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 ? A second example, immobilized insulin (Ito): null This data interestingly shows several biophysical effects: o PEG-insulin not as good as free insulin null Steric interference o PAA-insulin better than free insulin null Multivalent o Surface-immobilized PAA-insulin better than all above null Lack of internalization/signal downregulation? null ? Issues faced in incorporation of cytokines in biomaterials: null Protein stability (rugged, but not as good as peptides- may significant secondary structure to worry about null Steric interference of tether/surface with receptor binding ? Growth factors that have been studied in biomaterials: null EGF null Insulin (Y. Ito) null TGF-β (West) Lecture 4 – Biological Recognition pt. 2 8 of 9 BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 References 1. Voet & Voet. in Biochemistry. 2. Paredes, N., Rodriguez, G. A. & Puiggali, J. Synthesis and characterization of a family of biodegradable poly(ester amide)s derived from glycine. Journal of Polymer Science, Part A: Polymer Chemistry 36, 1271-1282 (1998). 3. Fan, Y., Kobayashi, M. & Kise, H. Synthesis and biodegradability of new polyesteramides containing peptide linkages. Polymer Journal 32, 817-822 (2000). 4. O, S. C. & Birkinshaw, C. Hydrolysis of poly (n-butylcyanoacrylate) nanoparticles using esterase. Polymer Degradation and Stability 78, 7-15 (2002). 5. Ekblom, P. & Timpl, R. Cell-to-cell contact and extracellular matrix. A multifaceted approach emerging. Curr Opin Cell Biol 8, 599-601 (1996). 6. Chapman, H. A. Plasminogen activators, integrins, and the coordinated regulation of cell adhesion and migration. Curr Opin Cell Biol 9, 714-24 (1997). 7. Mann, B. K., Gobin, A. S., Tsai, A. T., Schmedlen, R. H. & West, J. L. Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering. Biomaterials 22, 3045-51 (2001). 8. West, J. L. & Hubbell, J. A. Polymeric biomaterials with degradation sites for proteases involved in cell migration. Macromolecules 32, 241-244 (1999). 9. Gobin, A. S. & West, J. L. Cell migration through defined, synthetic ECM analogs. Faseb J 16, 751-3 (2002). 10. Ito, Y. Tissue engineering by immobilized growth factors. Materials Science and Engineering C 6, 267-274 (1998). 11. Ito, Y. Regulation of cell functions by micropattern-immobilized biosignal molecules. Nanotechnology 9, 200-204 (1998). 12. Kuhl, P. R. & Griffith-Cima, L. G. Tethered epidermal growth factor as a paradigm for growth factor-induced stimulation from the solid phase. Nat Med 2, 1022-7 (1996). 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