Index A/B milk and yoghurt, 428 Abbe refractometer, 458 Accelerated ripening of cheese, 418 adjunct starters, 418 attenuated lactic acid bacteria, 418 elevated ripening temperature, 418 exogenous enzymes, 418 genetically modified starters, 418 Acetyl CoA, , 81, 82, 87, 276 Acetyl CoA carboxylase, 98 Acetylcholine, 276 N-Acetyl-glucosamidase, 247 N-Acetylglucosarnine, 327 N-Acetyl-CI-D-glucoaminidase, 318, 328 Acid-base equilibria in milk, 449, 451 Acid casein, 211, 212, 213 Acid-coagulated cheeses, 419 see also Casein fermentation of lactose, 419 fresh acid-curd cheeses, 419, 420 Acid coagulation of milk, see Isoelectric Acid degree value (ADV), 108 Acid milk gels, 455-6 Acidophilus milk, 428 Acid phosphatase, see Phosphatases, Activity coefficient, 449 Adenosine triphosphatase, 98, 333 Age gelation of UHT milks, 317, 321, 374 Age thickening of cream, 117 Agglutination Aldolase, 98, 333 Alkaline phosphatase, see Phosphatases Alkan-2-ones (methyl ketones), 408 Alnarp process, 137 Amino acids coagulation of milk see also Milk fat globules, 455 essential, 201 non-essential, 201 8-Aminoalanine, 367 Aminopeptidase, 320 Amorphous calcium phosphate, 185 a-Amylase, 333 8-Amylase, 333 Analogue cheese, see Cheese analogues Analytical ultracentrifugation, 155 Antioxidant effect of sulphydryl groups, 377 Antioxidants, 265 Annatto, 460 Apo-lactoferrin, 364 Apparent viscosity, 454, 455 Arachidonic acid, 142 Arteriovenous difference studies, 201 Arthrobacter spp., 407 Attrition dryers, 21 1 Ascorbic acid (vitamin C), 132,265,289-90, 315 concentration in milk and dairy products, 290, 292, 293 deficiency syndromes, 290 dehydroascorbate, 289 2,3-diketogluonic acid, 289 function, 290 oxidation of, 440-2 reducing agent, 290 role as antioxidant, 290 RDA values, 290 RNI values, 290 stability, 290 Ash, 239,240 Aspartyl (acid) proteinase, 384 tertiary structure, 385 see also Chymosin; Cathepsin D Aspergillus niger, 340 Auto-oxidation, 134 Avidin, 28 1 Babcock butyrometer, 118 Bactofugation, 113, 382, 406 Bakery products, 220 Beriberi, 276 Bicarbonate, 239 Bijidobacterium spp., 43, 45, 49, 231 Biologically active proteins, peptides, 146, 228 angiotensin converting enzyme (ACE) inhibitor, 234 bifidus factors, 231 calrnodulin-binding peptide, 234 glucose oxidase, 229 growth factors, 231 bornbasin, 231 fibroblast growth factors, 231 IFG2, 231 IGF1, 231 464 INDEX Biologically active proteins, (conr.) mammary-derived growth factors, platelet-derived factor, 231 transforming growth factors, 231 23 1 lactoperoxidase, 229 nutraceutical, 23 1 vitamin-binding proteins, 230 xanthine oxidase, 229 see also Immunoglobulins; Vitamin- binding proteins; Metal binding proteins; Protein hormones; Caseinomorphins; Immunomodulating peptides; Platlet modifying peptides Biotin, 265, 275, 281 binding proteins, 281 concentration in milk and dairy products, 28 1 deficiency, 281 food sources, 281 intake, 281 RDA value, 281 role in gluconeogenesis, 281 ‘Bitty cream’defect, 117, 118, 317 Bixin, 131, 460 Blood serum albumin (BSA), 187, 195 binding of fatty acids, 195 binding of metals, 195 stimulation of lipase activity. 195 Blue cheese, 76 Boiling point of milk. 437, 443 Brassica. 332 Braunauer-Emmett-Teller model, 307, 308 Brevibacterium linens. 407, 432 Brie, 406 Brushite, 259 Buffering capacity, 450, 451 bicarbonate, 450 citrate, 450 soluble calcium phosphate, 450 Buffering curves, 452 Buffering index, 450 Bulk density of milk powders, 458 Butanoic scid, 87, 142 Butter, 78 blending, 138 blending with vegetable oils, 138 polyunsaturated fatty acids, 139 buttermaking, 118, 119, 125 butter churns, 123 churning, 118 phase inversion, 121 frozen cream, use of, 126 hardness of, 90, 134 lactic butter, 120 diacetyl in, 120 seasonal variations in firmness, 78 structure of butter, 120, 121, 456 sweet-cream butter, 120 water droplets in butter, 121 Buttermilk, 71 Buttermilk, cultured, 429 Butter oil, 120, 126 Butylated hydroxytoluene, 132 Butyrophilin, 94, 95, 102 Caking of whey and milk powders, 33 Calciferols (vitamin D), 269 cholecalciferol (vitamin DJ, 269, 270 deficiency of, 271, 272 osteomalacia, 271 rickets, 271 7-dehydrocholesterol, 269, 270 1,25-dihydroxycholecalciferol, 271 effect on plasma calcium, 271 ergocalciferol, 271 25-hydroxycholecalciferol, 270 hypervitaminosis D, 272 metabolites of vitamin D,, 271 pre-vitamin D,, 269 principal sources, 272 RDA values, 271 RNI values, 271 stability, 272 Calcium, 239, 241, 253, 261-2 measurement of, 254 Calcium-ion electrode, 256 Calcium phosphate, 38, 453 Camembert, 215, 406 Carbon tetrachloride, 118 Carbonate, 239, 241, 251 Cardiolipin, 143 Carotenoids, 73, 74, 97, 131, 132, 266, 268, P-carotene, 73, 74, 131, 266, 267, 268 amino acid composition, 163, 164 applications of caseins, 219 association, 180 chemical composition, 163 degree of phosphorylation, 160 disulphide bonding, 160 electrophoresis of, 159 functional (physicochemical) properties of caseins, 218 gelation of, 218 genetic polymorphism (variants), 162 heterogeneity and fractionation, 155, 163 heterogeneity of bovine casein, 163 homology, 171 hydration of, 218 hydrolysis of primary caseins by plasmin, hydrophobicity, 178 industrial fractionation of casein, 216 269,459, 460 Casein, definition, 149 160 INDEX 465 industrial production of caseins, 21 1 enzymatic (rennet) coagulation, 21 1 isoelectric precipitation, 21 1 influence of Ca2 * on caseins, 179 microheterogeneity of the caseins, 160 models of tertiary structures, 176 molecular size, 178 nomenclature of the caseins, 162 phosphorus in, 171 preparation acid (isoelectric) precipitation, 152 cryoprecipitation, 154 gel filtration (gel permeation) novel methods for casein production, precipitation with ethanol, 154 rennet coagulation, 154 salting-out method, 153 specific absorbance, 157 ultracentrifugation, 153 primary structures, 165-8 rennet action on casein, 179 rheological properties, 218 solubility, 218 surface activity, 219 variations in the degree of glycosylation, chromatography, 154 215 161 a-Casein, 155 a,,-Casein, 161, 165, 217 see also Casein a,,-Casein, 161, 217 see also Casein /?-Casein, 155, 160, 217 see also Casein y-Casein, 155, 160, 187, 217 see also Casein K-Casein, 150, 161, 183, 217 see also Casein Caseinate ammonium, 21 1 calcium, 21 1 potassium, 21 1 sodium, 211, 214, 310 Casein gels, 456 Casein kinase, 206 Casein micelles, 109, 153, 310 characteristics of casein micelles, 181 composition and general features, 180 cryodestabilization, 182, 215 destabilization, 39 electrostatic intractions, 184 electron microscopy, 184 models of micelle structure, 184 core-coat, 184 internal structure, 184 subunit (submicelles), 184 principal micelle characteristics, 183 role of colloidal calcium phosphate stability, 182 structure, 180 submicelle model of the casein micelle, 184 hairy layer, 185 hydrophobic bonds, 185 protruding K-casein hairs, 186 zeta potential. 185 surface (zeta) potential, 184 (CCP), 182, 183 Caseinomorphins, 233 Catalase, 128, 131, 247, 98, 318, 319, 330, 331, 336,339 destruction of hydrogen peroxide, 339 role as pro-oxidant, 331 Cathepsin D (acid milk proteinase), 320, 322 Cell-wall associated proteinase, see Lactococcus Ceramides, 71, 143 Cerebrosides, 71, 95, 96, 97, 143 Cheddar cheese, 399 flavour, 330, 339 Cheese, 379 Appellation d'origine Protegee status, composition, 380 consumption, 436 conversion of milk to cheese, 382 principal families, 379 rennet-coagulated cheeses, 380 world production, 435 see also Processed cheese products, 379 381 Cheese analogues, 427 Cheese flavour, 416 amines, 4 16 amino acids, 416 biogenesis of flavour compounds, 418 dimethyl sulphide, 418 dimethyl disulphide, 418 gas liquid chromatography (GC), 416 H,S, 418 mass spectrometry (MS), 416 methanethiol, 418 organic acids, 416 peptides. 416 role of water-soluble fraction, 416 taste of cheese, 416 volatile compounds in Cheddar cheese, see also Cheese ripening Cheese manufacture, 382-401 cheddaring, 399 milling, 399 moulding, 397 salting, 398 shaping, 397 41 7 466 INDEX Cheese manufacture (cont.) treatment of cheesemilk, 380 use of NaNO,, 382, 400 whey drainage, 399 see also Cheese starters and acidification: Rennet coagulation of milk Cheese ripening, 403 assessment of ripening fluorescamine, 410 high performance ion-exchange chromatography, 410 ninhydrin, 410 phosphotungstic acid, 410 o-phthaldialdehyde, 410 reversed-phase HPLC, 410 trinitrobenzene sulphonic acid, 410 urea-polyacrylamide gel electrophoresis, 410 water-soluble nitrogen, 41 1 deamination, 408 decarboxylation, 408 degradation of a,,-casein, 410-12 degradation of B-casein, 410, 412 desulphuration, 408 glycolysis, 404 lipolysis during, 407 production of amines, 409 production of bitter peptides, 408 production of butyric acid, 406 production of large polypeptides, 409 production of sulphur compounds, 409 production of yl-, 7’- and y3-caseins, 410 proteolysis during, 408 role of coagulant, 403 role of Maillard reaction and Strecker degradation, 408 role of phosphatase, xanthine oxidase, 403 role of plasmin, 403, 412 textural changes, 408 see also Cheese flavour; Accelerated ripening of cheese Cheese starters and acidification, 395 see also Lactococcus; Luctobacillus acidification, 394 galactose-positive/negative species/ homofermentative, 395 Leuconostoc spp., 395, 397 metabolism of lactose by, 396 pH profile during Cheddar cheese Slreptococcus sakarius ssp. thermophilus, strains, 405 manufacture, 395 395 Chloride, 239, 241 Cholesterol, 71, 73, 95 Cholinesterase, 98 cholesteryl esters, 71, 73, 80, 83 Churning, see Butter Chylomicrons, 85, 87, 100 Chymosin, 149, 183, 322, 383, 384,410 see also Rennets; Rennet coagulation of Citrate, 239, 241, 251, 254,. 261, 373, 445 Clausius-Clapeyron equation, 302,303,304 Clostridiurn tyrobutyricum, 339, 382, 406 Cobalamin, see Vitamin B,, Co-enzyme A (CoA), 281 Colligative properties of milk, 443, 444 Colloidal calcium phosphate (CCP), 150, milk 152, 171,257,259, 260-2,374, 451, 457 association with casein, 257 CCP-free milk, 257 composition and structure, 257 human, 229 74,459 benzoyl peroxide, 75 chlorophyll, 75 titanium oxide, 75 see also Carotenoids Colostrum, 75, 209, 328, 450 Colour of milk, milk fat, milk products, 73, Comte cheese, 394 Conalbumin, 229 Conjugated linoleic acid, isomers, 351-2 Co-precipitates, milk protein, 220, 221, 227 Co-translational modifications, 206 Cottage cheese, 420 Coulter counter, 90 Cream cheese, 420 Creaming, 104-8, 117 creaming properties of milk, 113 cryoglobulins, role in, 107, 108, 113, t 17 electrokinetic potential, 107 immunoglobulins, role in, 107 Cream plug, 117, 350 Cryoglobulins, 349 see also Creaming Cryoprecipitation of casein, 215 Cryoscopy, 447 Crystallization behaviour of milk Cultured buttermilk Cultured milks, 428 adulteration of milk, 447 triglycerides, 454 role of diacetyl, citrate, 429 see also Fermented dairy products, yoghurt Cysteine, 440 Cytochromes, 128, 131 Cytoplasmic crescents, 102 Cytoplasmic lipid droplets, 99 Cytoskeletal elements, 99 Deamination of amino acids, 408 INDEX 467 Decanting centrifuge, 21 1 Decarboxylation of amino acids, 408 Dehydroalanine, 367, 368 Dehydroascorbate, 289, 440 Density of milk, 437, 438 Desulphuration of amino acids, 408 Diacylglycerols (diglycerides), 71, 97, 449 Dialatometry, 439 2,6-Dichlorophenol indophenol, 441 2,3-Diketogulonic acid, 440 Diisopropylflurophosphate, 321 Dispersibility/wettability of milk powder, Dissolved oxygen in milk, 440 Dictyosomes, 101 Direct acidification of dairy products, 341 Domiati cheese, 398 Dutch-type cheese, 113 33,351 Ectopic mineralization, 186 Eiler’s equation, 453 Electrical conductivity of milk, 456, 457 Electrodialysis, 187, 224 Electron microscopy, 102 Electrophoresis, see Polyacrylamide gel 8-Elimination, 368 Emmental cheese, 394, 399 Emulsifying salts, see Processed cheese Emulsion, milk fat, 437, 459 electrophoresis products aqueous colloidal continuous phase, 437. oil/fat dispersed phase, 437 459 Enzyme-linked immunosorbent assays (ELISA), 343 authentication of cheese, 344 competitive ELISA, 343 detection of enzymes from psychrotrophs monitoring proteolysis, 345 noncompetitive ELISA, 343 quantification of denaturation of 8- lactoglobulin, 344 use of ELISA in dairy analyses, 344 Enzyme-modified cheeses, 418 Enzymes in milk and milk products, 317-46 344 enzymes of psychrotrophs, 317, 321 exogenous enzymes, 317, 333 exogenous enzymes in food analysis, 342 enzyme electrodes, 342 examples of compounds analysed, lactic acid, 342 luciferase, 342 indigenous enzymes, 317, 318 minor enzymes, 334, 335 use in accelerated cheese ripening, 336 342 use of exogenous proteinases and use of in modification of protein peptidases, 337 functionality, 337 Epilactose, 353 Erythrocyte haemolysis, 273, 274 Essential fatty acids, 67, 78 Esterase, 322 see also Lipase A-type carboxylic ester hydrolases B-type esterases (glycerol tricarboxyl (arylesterases), 322 esterases, aliphatic esterases, lipases), 322 C-type esterases (cholinesterase), 322 Ethanol, 50 Ethylene diamine tetraacetic acid (EDTA), 240,256,459 Faraday constant, 440 Fat, see Lipids in milk Fat content of milks, 67 mastitic infection, effect of, 68 milk yield, effect of, 69 seasonality, effect of, 68, 70 Fat globules, see Milk fat globules Fat globule membrane, see Milk fat globule membrane Fat-soluble vitamins (A, D, E, K), 67,73, 74, 75, 97, 265, 266, 268, 269, 270, 211,272, 273, 274, 275 see also Vitamins 8-keto acid, 87 cis isomers, 77, 137 6-hydroxy acid, 87 elongation/desaturation of fatty acids, 86 fatty acid distribution, 90 hydroxy acids, 77, 351 keto acids, 77 lactones, 351 malonyl CoA pathway, 77, 81, 82, 87 medium-chain, 77 melting point, 77 methyl ketones, 77 sources of fatty acids, 84 synthesis, 81 Fatty acids, 76 acetate, role in, 81, 85 acetyl CoA-ACP transacetylase acetyl CoA carboxylase, 82 acyl carrier protein (ACP), 82, 83 ATP citrate lyase, 81 8-hydroxyacyl-ACP-dehydrase 8-hydroxyburyryl-S-ACP, 82 8-keto-ACP synthase (KS), 83 j?-ketoacyl-ACP reductase (KR), 83 (AT), 83 (HD), 83 468 INDEX Fatty acids (cont.) enoyl-ACP reductase (ER), 83 malonyl CoA-ACP transferase pentose pathway, 82 role of blood glucose in, 81 (MT), 83 thioacylases, 77 trans isomers, 77, 137 type of fatty acid in triglycerides and esterification position, 89 Fatty acid lactones, 81 Feathering, 1 17 Fermented dairy products, 22 Flavin adenine dinucleotide (FAD), 277, Flavin mononucleotide (FMN), 277, 278 Flocculation, see Milk fat globules Fluidized bed dryer, 33, 21 1 Fluorescence, 459 Folate, 265, 275, 285 concentration in milk, 286 deficiency, 286 dietary sources, 286 folate-binding proteins, 287 interaction with vitamin B,,, 285 5-methyltetrahydrofolate, 285 oxidation to p-aminobenzoglutamic acid, RDA values, 286 stability, 286, 287 structure, 285 tetrahydrofolate, 285 see also Cheese; Cultured milks; Yoghurt 278 286 protection by antioxidants, 286 Folkjolk, 428 Forewarming (for sterilization), 347 Formograph, 389, 390, 392 Formol titration, 259 N-Formyl methionine, 204 Fourier equation for heat conduction, 457 Fractional crystallization of milk fat, 138 Fractionation, 137 Free boundary electrophoresis, 155 Free (non-globular) fat, 1 17, 1 18, 126 Free fatty acids in cheese, 408 Freezing point depression, 444 effect of chloride, 444 effect of HTST pasteurization on, 444 effect on UHT processing, 445 measurement, 447 seasonality, 444 vacuum treatment, 445 effect of added water, 437 Freezing point of milk, 437, 443, 444, 445 Fromage frais, 419 Fructose, 21 Functional milk proteins, 210 Furfural, 352 Galactosaemia, 56, 61 Galactosamine, 21, 56 Galactose, 62, 65, 353 Galactose- 1-phosphate (Gal-1 -P):uridyl Galactokinase, 60 Galacto-oligosaccharides, 43 metabolism, 61 transferase, 61, 62 b-Galactosidase, 38,42-5, 56, 58, 60, 65, 98, 317, 332. 336, 338. 341 Galactosyl transferase, 98 Gangliosides, 71, 96 Gel filtration, 224 Geotrichum candidum, 118 Gerber butyrometer, 118 Ghee, 120 Gibb’s equation, 448 Glass transition, 3 1 1, 3 12 changes that occur on glass transition, viscosity of lactose in the glassy state, 313 311 Gluconic acid-d-lactone, 381, 419 Glucosamine, 21 Glucose, 21, 59, 65 Glucose-6-phosphatase, 98 Glucose-fructose syrups, 39 Glucose-galactose syrups, 43 Glucose oxidase, 332, 336, 340, 341 P-Glucosidase, 98 j-Glucuronidase, 3 3 3 .~-Glutamyl transpeptidase (transferase), 98, 318, 328 Glutathione peroxidase, 333 Glycomacropeptides, 232, 382 Glycosylation, 206 N-Glycosylation, 205 0-Glycosylation, 205. 206 Golgi apparatus, 100. 101 Gouda cheese, 399 Growth factors, see Biologically active Gruggenheim- Andersson-De Boer model, protein, peptides 307, 308 H, electrode, effect of pH on redox potential, 441 Halloumi cheese, 398 Heat-acid coagulated cheese, 419 Heat-induced changes in milk, 347 association and shattering of casein changes in hydration, 371 changes in surface (zeta) potential, 371 chemical changes, 351 micelles, 371 dephosphorylation, 355 INDEX 469 interesterification, 351 loss of co,, 355 precipitation of Ca,(POJZ, 355 production of acid, 355 production of organic acids, 353, 354 proteins, 363 371 denaturation of other biologically active denaturation of whey proteins, 363-5, dephosphorylation of casein, 371 effect on caseins, 368 effect on milk salts, 359 effect on rennet coagulation of milk, 373 enzymes, 360 exposure of sulphydryl groups, 367 heat-induced changes in flavour of milk, hydrolysis of caseins, 371 microbial enzymes, 361, 362 physicochemical changes, 349 376 changes to the fat globule membrane, 349, 350 effect on creaming, 349 effect on lipids, 349 sulphydryl-disulphide interchange, 365 temperature dependence of reactions, 348 vitamins, 360 effect of pH, 370 Heat stability of milk, 369 additives, 373 concentration, 372 forewarming (preheating), 372 homogenization, 372 precipitation of soluble calcium phosphate. 371 role of pyrolysis of lactose, 370 type A and type B milks, 370 Henderson-Hasselbalch equation, 251, 450 Hexokinase, 65 High performance liquid chromatography Homogenization of milk, 108, 113-17, 126, denaturation of immunoglobulins, 114 FGM of homogenized milk, 114, 116 foaming, 116 light scattering, 11 5 Hormones in mammary development, 7 Hortvet cryoscope, 446 Hydrocolloids, 455 Hydrolytic rancidity, 68, 108, 133, 317 Hydrometer (lactometer), 438, 439 Hydrophilic interactions, 298 Hydrophobic interaction chromatography, Hydrophobic interactions, 298 j-Hydroxyanisole, 132 Hydroxymethyfurfural, 352 (HPLC), 155, 354, 392 131,449,454,455 155 j-Hydroxybutyrate, 75, 81, 82, 85 Hypoxanthine, 332 Ice, 294, 297 basal planes, 297, 299 crystals, 298 non-equilibrium ice formation, 312 physical constants, 296 structure, 297, 300 unit cell, 297 metastable lactose, 37 spontaneous lactose crystallization in, 37 Immobilized enzymes, 43 Immunoglobulins, 146, 187, 195, 196, 198, Ice-cream, 37 230, 449 concentration in colostrum, 195 in utero transfer, 197 role in immunity, 196 secretion of immunoglobulins, 209 structure, 196 Immunomodulating peptides, 233 Indigenous milk lipase, 449 ‘Instantized’ milk powder, 33 Interfacial tension of milk, 447, 448 International Dairy Federation (IDF), 13 Iodine number of milk fat, 77, 79 Ion-exchange chromatography, 155, 187, Ionic strength of milk, 437, 438 Isoelectric (acid) coagulation of milk, 379 224 Kefir, 428,429 Kjeldahl, 151 Kluyveromyces marxianus, 429 Koestler number, 21, 22, 247 Koumiss, 429 Kuhn model, 307, 308 Labaneh, 419 Labnah, 419,429 Lactalbumin, 187 I-Lactalbumin, 150, 187, 192 manufacture of, 223 amino acid composition, 192 calcium content, 194 genetic variants, 192 heat stability of, 194, 364 influence on heat stability of milk, 369 metal binding, 194 methods for isolation, 226 primary structure, 192 quaternary structure, 193 role in biosynthesis of lactose, 194 role in control of osmotic pressure, 194 secondary structure, 193 specifier protein, 194 470 INDEX Lactic acid, (cont.) tertiary structure, 193 Lactase, 42 see also P-Galactosidase Lactenins, 332 Lactic acid, 50, 120, 445, 451 Lactic acid bacteria, 428 effect of pH on redox potential, 441 see also Lactococcus; Non-starter lactic acid bacteria: Cheese starters and acidification Lactitol, 48, 51 Lactobacillus spp., 397, 432 Lactobionic acid, 48 Lactococcus, 211, 412, 419 peptides, 412 action of enzymes on casein-derived aminopeptidase, 412 cell wall-associated proteinase, 412 dipeptidase, 412 intracellular endopeptidase, 412 proline-specific peptidases, 412 tripeptidase, 412 Lactococcus lactis ssp. lactis biovar diacetylactis, 397 citrate metabolism by, 430 amino acid composition, 188 denaturation, 192 genetic variants, 188 effect on heat stability of milk, 369 heat stability of, 364 hydrophobic interaction, 190 methods for isolation, 226 occurrence and microheterogeneity, 188 physiological function, 191 primary structure, 189 quaternary structure, 190 /?-Lactoglobulin, 132, 150, 183, 187, 188,442 effect of pH on quaternary structure, 191 191 relationship to retinol (vitamin A), 190, secondary structure. 189 tertiary structure, 189 Lactometer, see Hydrometer Lactonase, 340 Lactoperoxidase, 131, 199, 318, 319, 331-3, 336, 341, 363 index of flash and super-HTST pasteurisation, 331 index of mastitic infection, 332 non-enzymic oxidation, 332 significance of lactoperoxidase, 332 1-lactose, 23, 24 Lactose, 11, 14, 17, 21-66 anhydrous, 30 monohydrate, 29 sandiness, 30 fi-lactose, 23, 24 biosynthesis, 23 anhydride, 30 blood, 23 galactosyl transferase, 23 Leloir pathway, 23 osmotic pressure, 23 role of a-lactalbumin, 23 UDP-galactose, 23 chemical modifications, 43 concentration in milk, 21 crystallization, 28 nucleation, 28 crystal shape, 27 derivatives of lactose, 42 anthrone, 64, 65 chloramine-T, 63 chromatographic methods, 65 chromatography, 62 colorimetry, 62 enzymatic methods, 62, 65 Fehling’s solution, 63, 64 oxidation-reduction titration, 62 phenol, 64 polarimetry, 62 effect of temperature on solubility, 27 fermentation products, 50 food applications, 41 heat-induced changes in, 352 hygroscopicity, 27, 31, 32, 33 intolerance, 23, 56, 58, 59 lactose glass, 31 mutarotation, 25 nutritional aspects, 56-62 physical properties, 31 production, 39, relative humectancy, 42 relative sweetness, 41, 42 solubility, 27 determination of concentration, 62 supersaturation, 29, 38 unsaturated, metastable, labile zones, 28 specific rotation, 25 structural formulae, 24 structure, 23 thermoplasticity, 35 Lactosyl urea, 50 Lactotransferrin, 187, 229, 363 bacteriocidal peptides from lactotransferrin, 234 Lactulose, 43, 46-8, 353 as bifidus factor, 45 cariogenicity, 45 effect on intestinal microflora, 48 formation, 354 production during sterilization, 45 Lactyl palmitate, 51 INDEX 471 Lanthionine, 367 consequences for intra- or intermolecular cross-linking, 368 Laplace principle, 104 Late gas blowing in cheese, 406 Lecithin, 143 Leucocytes, 116 Light-activated flavour, 108 Limburger cheese, 407 Linoleic acid, 67, 87, 142 Linolenic acid, 87, 142 Lipase, 110, 199, 317-19,322,323,336, 338 activation by blood serum albumin and Ca2', 322 gastric, 339 microbial, 339 pancreatic, 323 technological significance, 323 see also Lipoprotein lipase see also Microlipid droplets addition of fish oils, 69 classes of lipids, 7 1 crystalline form of fat, 137 effect of dehydration, 126 de-emulsification of milk fat, 127 dispersibility, 127 wettability, 127 see also Somatic cells Lipid droplets, 99-100 Lipids in milk, 67 effect of freezing, 126 lipids of marine mammal milks, 77 monogastric milk fat, 77 principal fatty acids in milk, 76 ruminant milk fat, 69 structure of milk lipids, 87, 90 see also Fatty acids, 67 Lipid oxidation, 127, 133 antioxidants, 129, 132 autolcatalysis, 129, 132 autooxidation of fatty acids, 128 compounds contributing to oxidized flavour, 130 effect on polyunsaturated fatty acids (PUFA), 127 factors affecting rate of, 131 free-radical chain, 127 hydroperoxides, 129 initiation, 127 measurement, 134 chemiluminescence, 134 Kreis test, 134 peroxide value, 134 thiobarbituric acid value (TBA), 134 propagation, 127 rate of oxidation, 130 role of peroxy radicals, 129 termination, 127 Lipoamide dehydrogenase, 98 Lipolysis in milk, 323, 449 Lipoprotein co-factor, 110 Lipoprotein lipase, 85, 109, 361 Lipoprotein particles, 85 high density lipoprotein (HDL), 85 low density lipoprotein (LDL), 85, 87 very low density lipoprotein (VLDL), 85, 87, 100 Lipoxygenase, 129 Listeria spp., 3 15, 339 Lobry de Bruyn-Alberda van Ekenstein Loss modulus (G"), 455 Low-fat spreads, 139 Low-lactose milk, 60 Low-temperature inactivation of enzymes, rearrangement, 352 362 Lumiflavin, 279 Lysinoalanine, 367 Lysosomes, 101 Lysozyme, 318, 319, 327, 336, 339, 363 action on mucopolysaccharides, 327 egg-white lysozyme, 327 human milk lysozyme, 327 significance, 327 Machine milking, 109 Macropeptides, see Glycomacropeptides Magnesium, 239, 241, 253 measurement of, 254 Maillard browning, 32, 57, 131, 134, 350, 356, 357, 358, 360, 371, 377, 313, 314, 442 effect on available lysine, 357 glycosylamine, 56 in Mozarella (Pizza) cheese, 356 production of carboxymethyl lysine, 357, 358 2,4-dinitrofluorobenzene, 357 erythronic acid, 358 fructosylysine, 357, 358 furfural, 357 furosine, 357, 358 hydroxymethyfurfural, 357 melanoidins, 356 pyridosine, 357, 358 strecker degradation, 58, 357, 360 Malvern Mastersizer, 90, 116 Mammals classification, 3 eutherians, 3 marsupials, 3 prototheria, 3 alveoli, 4, 11 blood, 4, 8 effect of hormones, 4, 6 Mammary gland, 3, 5, 6, 8-10, 450 472 INDEX Mammary gland, (cow) effect of mastitis on permeability of, 450 endoplasmic reticulum, 5, 7 Golgi apparatus, 5, 7 lumen, 4 lysosomes, 8 mammocytes, 4-5, 100 mitochondria, 5, 7 secretory cell, 6, 7 uptake of blood constituents, 84 r-Mannosidase, 333 Mastitis, I, 21, 247, 328, 331, 450 Membrane lipase, 323 Membrane processing of milk proteins, 216 Menadione (vitamin K3), 275 Menaquinone (vitamin K2), 274, 275 Mercaptans, 377 Melting point of fat, 136, 139 Mesophilic lactobacilli, see Non-starter Metal-binding proteins, 146 Metal-catalysed oxidation, 108, 115 Methional, 133 Methionine, 132 Methylene blue, 441, 443 Micrococcus spp., 340. 404, 407 Microfilaments, 100 Microfiltration, 382, 406 Microfixation, 137, 138 Microlipid droplets, 99 Microsomes, 103 Microtubules, 100 Milk fat, see Lipids in milk Milk fat emulsion lactic acid bacteria demulsification, 105 emulsion interfacial area, 113 interfacial tension, 104 stability, 104, 105 agglutination, 107. 108 average diameter, 91 clustering of, 106 coalescence, 105 emulsifiers, 92, 104 flocculation, 105 interfacial area, 92 interfacial tension, 92 milk fat as an emulsion, 90, 92 Coulter counter, 90 light microscopy, 90 light scattering, 90 Malvern Mastersizer, 90, 116 Milk fat globules, 90, 98, 99, 107, 116. 117 number, 91 secretion, 100 Milk fat globule membrane (MFGM), 71, 92, 93, 94, 318, 323, 329 composition, 94 cytochrome, 96 enzymatic activities, 98 fluid-mosaic model, 103 free fatty acids, 96 glycoproteins of, 94, 102 hexosamines, 96 hexoses, 96 influence of processing operations, 108 isolation, 93 lipid fraction of, 95 membrane structure, 97 metal ions of, 96, 11 5 origin as Golgi membranes, 318 plasmin in, 94 pro-oxidants, 115, 128-30, 132 protein fraction of MFGM, 94, 102, 104, RNA, 96 trilaminar structure, 102, 103 uronic acids, 96 Milk and milk products composition and variability, 1-3 consumption, 12-15 butter, 13 cheese, 12 cream, 14 fermented milks, 15 liquid milk, 12 109, 113 SDS-PAGE Of, 94,99 diversity, 17 production and utilization, 1, 11 trade, 18-19 Milko-TesterTM, 459 Milk protein hydrolysate, 232 Milk serum, 260 Milk synthesis, 8 ATP, 11 UTP, 11 see also Lactose; Lipids in milk; Proteins of milk see also Enzymes Minor milk proteins, 199 Monoacylglycerols (monoglycerides), 71, Mozzarella cheese, 215, 394, 395, 398, 428 Munster cheese, 407 Murexide, 255 Mycobactcrium tuberculosis, 324 NADH oxidase, 98 NADPH oxidase, 98 Native casein, 216 Nernst equation, 439 Neuraminic acid, 21 Newtonian behaviour, 453 Niacin, 265, 275, 279, 280 85,90,449 concentration in milk and dairy products, 280 deficiency, 280 INDEX 473 RDA values, 280 RNI values, 280 stability, 280 synthesis from tryptophan, 279 nicotinamide adenine dinucleotide (NAD), 279 nicotinamide adenine dinucleotide phosphate (NADP), 279 see also Niacin Nitrate, 339 formation of nitrosamines, 339 Non-casein nitrogen, 149, 186, 199 concentration in bovine milk, 200 Non-globular fat, see Free fat Non-Newtonian rheological behaviour, 454 Non-protein nitrogen, 150 Non-starter lactic acid bacteria (NSLAB), 404, 41 1, 416 Nicotinic acid, nicotinamide mesophilic lactobacilli, 416, 418 Norbixin, 460 Nucleotidases, 98, 333 Nutraceuticals, 14 Oiling-off, 108, 117, 350 Oleic acid, 87, 97, 142 Omega-3 (w-3) fatty acids, 139 Organophosphates, 322 Ornithinoalanine, 367 Osmotic pressure, 21, 437, 443, 444 Ovotransferrin, 229 Oxalate titration, 257 P-Oxidation, 87, 408, 409 &Oxidation, 87 Oxidation-reduction potential, 441 Oxidative rancidity, 68 Palmitic acid, 142 Palmitoleic acid, 87, 142 Panthothenate, 265, 275, 282 concentration in milk and dairy products, 282 deficiency, 282 food sources, 282 RDA values, 282 RNI values, 282 stability, 282 Para-K-casein see also rennet coagulation of milk, 391 Parmigiano-Reggiano (Parmesan), 76, 394, Pasta filata cheese varieties, 398 Pasteurization, 449, 454 399 flash, 331 high temperature short time (HTST), 45, low-temperature, long time (LTLT), 347 super-HTST, 331 116, 117, 126, 134, 347 Pediococcus spp., 404 Pellagra, 280 Penicillium camemberti, 397 Penicillium candidum, 338 Penicillium glaucum, 340 Penicillium roqueforti, 77, 338, 397, 408 Peroxidase, 128, 330 Petit suisse, 420 pH of milk, 2. 437, 449, 450, 451, 454 Phenolphthalein, 451 Phenylmethyl sulphonyl fluoride, 321 Phosphatases acid phosphomonoesterase (acid phosphatase), 318,319,324,326-8 dephosphorylation of casein, 326 dephosphorylated peptides in cheese, significance, 326 similarity to phosphoprotein 326 phosphatase, 326 alkaline phosphomonoesterase (alkaline phosphatase), 318, 319, 324, 326 characteristics, 325 dephosphorylation of casein, 325 index of pasteurization, 324 isolation and characterization, 324 reactivation, 325 phenolphthalein phosphate, 324 p-Nitrophenyl phosphate, 324 assay methods, 324 in fat globule membrane, 98 Phosphates, 239, 254, 261, 262 organic, 150 orthophosphates, 373 soluble, 171 Phosphatidic acid, 143 Phosphatidic acid phosphatase, 98 Phosphatidylcholine, 71, 80, 95, 96 Phosphatidylethanolamine, 71, 80, 95, 96, Phosphatidylglycerol, 143 Phosphatidylinositol, 96 Phosphatidylserine, 96, 143 Phosphodiesterase I, 98 Phospholipids, 71, 72, 73, 85,93, 95, 96,449 Phosphopeptides, 232 Phosphoric acid, 251 Phosphorus, 241 0-Phosphorylation, 205, 206 Phosphoserine, 259 Photo-oxidation, 443 Phylloquinone (vitamin Kl), 274, 275 143 concentration in milk, 275 jaundice, 274 menaquinone (vitamin K2), 274, 275 menadione (vitamin KJ, 275 osteocalcin synthesis, 274 prothrombin synthesis, 274 474 INDEX Phylloquinone (vitamin K,) (cont.) RDA values, 274 stability, 275 toxicity, 274 Physical properties of milk, 438 Piquant flavour, 407 Plasmin, 94, 98, 319, 320-1, 374 activity on milk proteins, 321 effect on cheesemaking properties of milk, 321 inhibitors, 321 importance in Swiss, Italian cheeses, 321 role in age gelation in UHT milk, 321 significance of activity in milk, 321 structure, 321 Plasminogen, 320 plasminogen inhibitors, 320 Plasminogen activators, 320 inhibitors of plasminogen activators, 320 Platelet-modifying peptide, 233 Polenski number, 75, 77 Polyacrylamide gel electrophoresis (PAGE), 157, 159,410 Polyenoic acids, 87 Poly-proline helix, 169 Polyunsaturated fatty acids, 69, 77 Post-transitional modification, 206 Potassium, 239, 241 Pregastric esterase, 338, 339, 407 Processed cheese products, 421 aluminium phosphate, 425 bacteriostatic effect of salts, 426 cheese base, 424 emulsifying agents, 424 non-cheese ingredients, 424 process cheese blends, 424 processed cheese, 424 pyrophosphate, 425 polyphosphate, 425 use of citrate, 425 orthophosphate, 425 Pro-oxidants, 329 Propionibacterium spp., 50, 397 Propionic acid, 50 Prostaglandins, 75 Proteinase, 199, 318, 322, 336 proteinases from psychrotrophs, 374 see also Plasmin; Cathepsin D; Thiol proteinase Protein hormones, 146 Protein hydrolysates, 337 bitterness in, 337 debittering with carboxylpeptidase, aminopeptidase, 337 Proteins of milk, 11, 14, 146 amino acid composition of major milk applications of milk proteins in food proteins, 164 products, 220 beverages, 220 convenience foods, 221 dairy products, 220 dessert products, 221 meat products, 221 pharmaceutical and medical products, 222 textural products, 221 changes in protein concentration, 146 comparison of human and bovine milk heterogeneity of milk proteins, 148 intracellular transport of proteins, 209 properties of some milk proteins, 158 proteins, 200,201 A,,,, 158 amino acids, 158 carbohydrate, 158 distribution, 158 hydrophobicity (kJ/residue), 158 intermolecular disulphide bonds, 158 mol % residues, 158 molecular weight, 158 net chargeiresidue, 158 number of residues/molecule, 158 phosphate, 158 species, 148 protein content in the milk of some secretion of milk-specific proteins, 207 structure and expression of milk protein genes, 206 synthesis and secretion of milk proteins, 201,203 sources of amino acids, 201 amino acid transport into mammary protein release factor, 205 signal sequence, 205 modification of the polypeptide chain, 205, 206 cell, 203 see also Whey protein; Caseins Proteolysis in cheese ripening, see Cheese ripening Proteose-peptone, 152, 187, 160, 186, 187, 32 1 Provolone cheese, 398, 407 Pycnometry, 439 Pyridoxine (vitamin B6), 265, 275, 282-3 concentration in milk and dairy products, 284 deficiency, 282 dietary sources, 284 pyridoxal, 282, 283 pyridoxal phosphate, 283 pyridoxamine, 282-3 pyridoxamine phosphate, 283 RDA values, 283 RNI values, 284 INDEX 475 role in niacin synthesis, 282 stability, 284 thiazolidine derivative of pyridoxal, 284 transaminase co-factor, 282 Pyrophosphatase inorganic, 98 nucleotide, 98 Quarg, 420 Rancidity, spontaneous, 323 role of membrane lipase, 323 Raoult’s law, 444 Redox potential of cheese, 397 Redox potential of milk, 2, 437, 439, 440, 439, 442 see also Oxidation-reduction Reducing sugar, 23 Refractive index of milk, 437, 458, 459 Reichert Meissl number, 75, 77 Relative density, see Specific gravity Relative humidity, equilibrium, 302 Rennet casein, 212 Rennet coagulation of milk, 379, 382 chymosin action, 383 coagulation of rennet-altered micelles, factors that affect rennet coagulation, 387, formation of para-rc-casein, 382 kinetic parameters for hydrolysis of measurement of rennet coagulation time, 386 388 K-casein, 384 387 Formograph, 389, 390, 392 gel strength, 389, 392 hot wire sensor, 391 pentrometers, 392 primary (first) phase, 383 rennet-coagulated cheeses, 380 secondary phase, 383 syneresis, 382, 392, 393 Rennet gels, 456 Rennet paste, 338, 403, 407 Rennets, 149, 317, 333, 336, 384 rennet substitutes, 385 Resazurin, 441, 443 Retinol (vitamin A), 73, 265, 266, 269, 315 fi-ionone ring, 266, 268 carotenoids, 268 concentration in human milk and deficiency, 268 colostrum, 268 keratinization, 268 night blindness, 268 xerophthalmia, 268 dietary sources, 268 hypervitaminosis A, 268 retinal, 266, 268 1 1-cis-retinal, 268 retinoic acid, 266, 268 retinyl esters, 266 retinyl plamitate, 267 Rhizomucor spp., 338 Riboflavin (vitamin B2), 265, 275, 277-9, 315,440,441,443,459 concentration in milk and dairy products, 279 deficiency, 277 flavoprotein enzymes, 277 RDA values, 277 RNI values, 277 role as co-enzyme precursor, 277 stability of riboflavin, 279 Riboflavin-binding protein (RfBP), 279 Ribonuclease, 98, 318, 324, 333 Ricotta cheese, 421 Ring dryer, 21 1 Rheological properties of milk, 453 Rheology of milk fat, 134, 456 effect of fatty acid profile and distribution, 134 modification by encapsulating fat in protein, 134 position of the double bond, 136 Rheology of milk gels, 455 Rheopectic (shear thickening) behaviour, 455 Romano cheese, 76,407 Rose Gottlieb method, 118 Salts of milk, 11, 239 changes in equilibria, 260 addition of acid or alkali, 260 effect of temperature, 261 changes in pH induced by temperature, 262 composition, 240, 241 factors affecting variation, 243 dissociation constants, 251 effect of dilution and concentration, 262 effect of freezing, 263 effect of temperature on pH, 262 interrelations of constituents, 247, 248 methods of analysis, 239 methods used to separate colloidal and partition of milk salts, 249 secretion, 242 Golgi apparatus, 242 solubility product, 260 soluble salts, 250 see also Phosphate; Citrate; Chloride; Sulphate; Carbonate; Bicarbonate; Sodium; Potassium; Calcium; Magnesium; Ash; Colloidal calcium phosphate soluble phases, 249 476 INDEX Seeding for lactose crystallization, 34 Separation of milk, mechanical, 11 1, 112 Serotransferrin, 187 Serum protein, see Whey protein Short-chain fatty acids, 75 Signal peptidase, 205 Signal sequence, 205 Signet, 102 Singlet oxygen, 128 Skim milk, 71 Skim milk powder, 457 Slop-back/whey cultures, 394 Sodium, 239, 241 Sodium caseinate, see Caseinate, sodium Soret band, 332 Spray dryers, 34 Specific conductance, 457 Specific gravity of milk, 437, 438 Specific heat of milk, 437, 457 Specific heat of milk fat, 457 Specific refractive index (refractive constant), 437. 459 Specific resistance, 456 Sphingomyelin, 71, 95, 96, 143 Sphingosine, 143 Spontaneous oxidation, 133 Spontaneous rancidity, 109 Squalene, 97 Staphylococcus aureus, 31 5 Starters, see Cheese starters and acidification Stearic acid, 87, 90, 142 Sterilization (UHT, in-container), 347, 354, Steroid hormones, 73 Sterols, 96, 97 steryl esters, 96, 97 see also Cholesterol Sticking temperature, 35 Stokes’ equation, 104, 106, 11 1 Storage modulus (G’), 455 Sweetened condensed milk, 36 Swiss-type cheese, 1 13 Sulphate, 239, 241 Sulphydryl oxidase, 318, 319, 330, 377 Sunlight oxidized flavour, 115, 133 Supercooling, 445 Superoxide dismutase, 318, 319, 330-1, 336, 34 1 centrifugal force, 113 356 effect of EDTA, 331 Superoxide radicals, 132, 330 Surface active agents (surfactants), 448 Surface tension of milk, 437, 449 Syneresis, 382, 392, 393 Taetmojolk, 428 Tagatose, 353 Talose, 353 Thermal conductivity of milk and/or Thermal diffusivity of milk, 437, 458 Thermal expansion coefficient of milk, 438 Thermal properties of milk, 457 Thermization, 347, 377, 381 Thiamin (vitamin B,), 265, 275-7, 315 products, 437, 458 concentration in milk, 276, 277 deficiency, 276 RDA values, 276 RNI values, 276 role in Krebs cycle, 276 sources, 276 thiamin pyrophosphate (TPP), 275-6 Thiobarbituric acid, 357 Thiol oxidase, 98, 330 Thiol proteinase, 320 Thixotropic (shear thinning) behaviour, 454 Thrombin, 320 Titratable acidity, 437, 451 Titration curves, 451 Tocopherols (vitamin E), 272-4 6-chromanol, 272 concentration in milk, 274 deficiency, 273 food sources, 273 hypervitaminosis E, 273 neuromuscular dysfunction, 273 RDA values, 273 RNI values, 273 role as antioxidant, 273, 279 free radicals, 273 protection of polyunsaturated fatty acids, 273 stability, 274 role of lipoxygenase, 274 role of pro-oxidants, 274 tocopherol equivalents (TE), 272 tocotrienol, 272 Transesterification, 90, 137 Transferrin, 229 Triacylglycerols, 71, 85, 87 Tricalcium phosphate, 257, 258 Trichloroacetic acid (TCA), 240 Triglycerides, see triacyglycerols Triplet oxygen (302), 129, 329, 443 UDP-galactosyl transferase, 194 UDP-glycosyl hydrolase, 98 UHT sterilized products biosynthesis, 88 see also sterilization, 134, 445 flavour of UHT milk, 375 UHT milk, 38, 45 Ultracentrifugation, 2 16 Ultrafiltration, 153, 187, 380, 381, 451 Urea, 200 INDEX 477 Utrecht phenomenon, 368 Vacreation, 377, 445 Viscoelastic modulus (G*), 455 Viscoelastic properties of milk fat, 456 Viscosity of milk, 437, 453, 454 coefficient of viscosity, 453 thermal hysteresis of, 454 Vitamins, 11, 265 B group, 275 role as co-enzymes, 275 vitamin B,, see Thiamin vitamin B,, see Riboflavin vitamin B,, see Pyridoxine vitamin A, see Retinol vitamin D, see Calciferols vitamin E, see Tocopherols vitamin K, see Phylloquinone vitamin and vitamin precursor concentrations, 292-3 water-soluble, 265, 275 vitamin C, see Ascorbic acid fat-soluble, 265 Vitamin A, see retinol Vitamin B,, (cobalamin and its B,,-binding proteins, 288 concentration in milk and dairy products, cyanocobalamin, 287 5’-deoxyadenosylcobalamin, 287 hydroxocobalamin, 287 methylcobalamin, 287 RDA values, 287 RNI values, 287 vitamin B,, deficiency, 287 stability, 288, 289 Vitamin-binding proteins, 146 Voluminosity, 454 Votator, 123 derivatives), 265, 275, 287-8 transcobalamin 11, 288 287 Water, 294-3 16 bulk-phase water, 300 bulk water, 298 constitutional water, 298, 301 free and bound, 298 interaction with functional groups, 301 rnultilayer water, 299 physical constants, 296 physical properties, 294, 296 polarity, 294 properties of water and other role in crystallization of lactose, 313 role in plasticization, 31 1, 312 role in stickiness and caking of powders, compounds, 296 313 structure of water, 296, 299, 300, 301 vapour pressure, 301 vicinal (monolayer) water, 299, 301 water-water hydrogen bonding, 301 Water activity (aw), 133, 294, 301, 302, 304, 313,437 control of the growth of microorganisms, 3 13 denaturation of proteins, 313, 315 effect on Maillard browning, 313-15 effect on rate of growth of microorganisms, 3 15 lipid oxidation, 3 13, 3 15 loss of certain vitamins, 313, 315 physical changes in lactose, 313 pigment stability, 313 measurement, 304, 305 cryoscopy, 304 hygroscopicity of various salts, 304 isopiestic equilibration, 304 manometry, 304 measurement of ERH, 304 nomograph for estimation, 305 relationship with temperature, 303 Water in milk and dairy products, 11, emulsification state in butter, 315 role in stability of dairy products, 313 water content of dairy products, 295 see also Water; Water activity; Ice absorption, 305 desorption, 305 models for isotherms. 307, 308 sorption behaviour of dairy products, 307 sorption by casein micelles and sodium caseinate, 309-11 sorption hysteresis, 306, 307 sorption isotherms, 306, 307, 308 sorption isotherm for P-lactoglobulin, 309 sorption by WPC, 309 water sorption by lactose, 31 1 water sorption by whey fractions, 308 influence on stability, 313 294 Water sorption, 305, 306, 31 1 Westphal balance, 439 Wettability of milk powders, see Dispersibility and wettability of milk powders Whey-based cheese, 419 Whey powder, 33 Whey proteins, 149, 186, 219 heat stability, 364 heterogeneity of whey proteins, 187 fractionation of whey proteins, 224 whole whey protein products, 222 see also u-Lactalbumin; 8-Lactoglobulin; Blood serum albumin; Immunoglobulins 478 INDEX Whey protein concentrate, 187, 337 Whey protein isolate, 187, 225 Xanthine oxidase, 94, 97, 98, 102, 115, 118, 128, 131, 133, 318, 319, 330, 332, 341 activity in milk, 329 effect of homogenization on activity, 329 isolation, 329 possible role in atherosclerosis, 330 role in lipid oxidation, 329 Xerophilic yeasts, 315 Yakult, 428 Yield stress, 456 Ymer, 428 Yoghurt, 59,428,429, 431 8-lactoglobulin denaturation in, 429 flavouring, 429 fruit essence, 429 fruit pulp, 429