7.2,GC Sample Preparation Guide
Overview,
This handout describes how to prepare a standard gas chromatograph sample,It involves
preparing a dilute solution of a somewhat volatile compound and using the GC to assess its purity,
Reference,
Zubrick page 252
Liquid Sample Preparation,
1) Insert the tip of a Pasteur pipet into the liquid,Capillary action will draw approximately
10 mg of the liquid into the pipet,
2) Rinse this into a vial using 1 mL of a volatile solvent – ether,ethyl acetate,pentane,etc,
3) Insert the tip of a pipet into this liquid,
4) Rinse this through a pipet filter into another vial using 1 mL of the same solvent,
5) Your sample is ready to be injected!
Solid Sample Preparation,
1) Dissolve approximately 10 mg of the compound in 1 mL of one of the volatile solvents
listed above,
2) Perform steps 3–5 above
46
WARNING NOTICE,The experiments described in these materials are potentially hazardous and require a high level
ofsafety training,special facilities and equipment,and supervision by appropriate individuals,You bear the sole
responsibility,liability,and risk for the implementation of such safety procedures and measures,MIT shall have no
responsibility,liability,or risk for the content or implementation of any of the material presented,Legal Notices
Overview,
This handout describes how to prepare a standard gas chromatograph sample,It involves
preparing a dilute solution of a somewhat volatile compound and using the GC to assess its purity,
Reference,
Zubrick page 252
Liquid Sample Preparation,
1) Insert the tip of a Pasteur pipet into the liquid,Capillary action will draw approximately
10 mg of the liquid into the pipet,
2) Rinse this into a vial using 1 mL of a volatile solvent – ether,ethyl acetate,pentane,etc,
3) Insert the tip of a pipet into this liquid,
4) Rinse this through a pipet filter into another vial using 1 mL of the same solvent,
5) Your sample is ready to be injected!
Solid Sample Preparation,
1) Dissolve approximately 10 mg of the compound in 1 mL of one of the volatile solvents
listed above,
2) Perform steps 3–5 above
46
WARNING NOTICE,The experiments described in these materials are potentially hazardous and require a high level
ofsafety training,special facilities and equipment,and supervision by appropriate individuals,You bear the sole
responsibility,liability,and risk for the implementation of such safety procedures and measures,MIT shall have no
responsibility,liability,or risk for the content or implementation of any of the material presented,Legal Notices
