The Digital Lab Techniques Manual
Created by Dr,Sarah Tabacco and Aayesha Siddiqui
MIT Department of Chemistry
1,Volumetric Techniques,Don’t let inaccuracy hold you back in lab! This video
introduces the proper methods for measuring precise volumes of liquid using
pipets,burets,volumetric flasks,and syringes,
2,Titration,Learn how to master the art of titration in this video––with a
detailed demonstration of an acid/base titration using phenolphthalein,
3,TLC-The Basics,Thin-layer chromatography is the most commonly used
analytical technique in many research labs,This video walks you through every
step,from setting up a developing chamber to calculating Rf values,
4,TLC-Advanced,Discover a few of the more complicated skills related to thin-
layer chromatography––cutting glass TLC plates,flame-pulling spotters,and
using TLC stains to visualize spots,
5,Reaction Work-Up I,Extracting,Washing & Drying,It ain’t over ‘til it’s over,
Learn how to,work up” your reaction using a separatory funnel to perform a
liquid-liquid extraction,This is one purification technique you don’t want to
miss!
6,Reaction Work-Up II,Using the Rotavap,The rotary evaporator is your friend
in the lab,This video will ensure that you build a safe and productive
relationship,
7,Filtration,The easiest way to separate a liquid from a solid? Filtration! Learn
how to effectively carry out gravity and vacuum filtrations in this video,
8,Sublimation,From solid to gas,and then straight back to solid,This
purification technique is both beautiful and useful,Find out why by watching
the atmospheric pressure sublimation of ferrocene in this video,
9,Recrystallization,Recrystallization takes patience,but it’s worth it! This video
walks you through the procedure,from solubility tests to cold filtration,with
information on one-solvent and two-solvent recrystallizations,
10,Column Chromatography,It takes considerable practice to master the art of
“running a column”,This video will get you started,with tips on picking
appropriate conditions,packing and running a column,monitoring separation,
and even making a micro-column from a pipet,
Created by Dr,Sarah Tabacco and Aayesha Siddiqui
MIT Department of Chemistry
1,Volumetric Techniques,Don’t let inaccuracy hold you back in lab! This video
introduces the proper methods for measuring precise volumes of liquid using
pipets,burets,volumetric flasks,and syringes,
2,Titration,Learn how to master the art of titration in this video––with a
detailed demonstration of an acid/base titration using phenolphthalein,
3,TLC-The Basics,Thin-layer chromatography is the most commonly used
analytical technique in many research labs,This video walks you through every
step,from setting up a developing chamber to calculating Rf values,
4,TLC-Advanced,Discover a few of the more complicated skills related to thin-
layer chromatography––cutting glass TLC plates,flame-pulling spotters,and
using TLC stains to visualize spots,
5,Reaction Work-Up I,Extracting,Washing & Drying,It ain’t over ‘til it’s over,
Learn how to,work up” your reaction using a separatory funnel to perform a
liquid-liquid extraction,This is one purification technique you don’t want to
miss!
6,Reaction Work-Up II,Using the Rotavap,The rotary evaporator is your friend
in the lab,This video will ensure that you build a safe and productive
relationship,
7,Filtration,The easiest way to separate a liquid from a solid? Filtration! Learn
how to effectively carry out gravity and vacuum filtrations in this video,
8,Sublimation,From solid to gas,and then straight back to solid,This
purification technique is both beautiful and useful,Find out why by watching
the atmospheric pressure sublimation of ferrocene in this video,
9,Recrystallization,Recrystallization takes patience,but it’s worth it! This video
walks you through the procedure,from solubility tests to cold filtration,with
information on one-solvent and two-solvent recrystallizations,
10,Column Chromatography,It takes considerable practice to master the art of
“running a column”,This video will get you started,with tips on picking
appropriate conditions,packing and running a column,monitoring separation,
and even making a micro-column from a pipet,
