1×0′?c?!¨í `DBge2? Gregory D.Schuler National Center for Biotechnology Information National Library of Medicine. National Institutes of Health Bethesda. Maryland é?? ü*ó(=:,X-è0JY?t *ü,X?"?E?E?!¨EWúd9? aY*ü,XμC `-1A??E?è[!7 -è0J!¨EWZ galapagos finches àJW?o(=/?,X6?:(MUè￠5à¤?Z7?' EY?:Aè? ?yè?àí ? ′ `<?,QBüc?Eˉ> !¨EWè￠BüT9A? àE?è[? èEˉ> à ,Xú dè ?áE?èt2?4?èètAo? üE-t?Tè?à￠ GL1 ?!ü ?GL,X ? ?údc? ,X,ì à&? `á à&?è1ó6?ó|#Wà,X4§X?s6?1 ?EˉêT,X6(2??? *ü,X!¨EW?"?  c?!¨í èW?têè?tc?,X!_ ?Kè,X,ìfG2?¤oZ?tM2 a.B,X òB? ü E-?0′è?à ?A|A??? ?G?!¨í è G ?!¨EW?tc?è7?bEW?,Xc? G ?c?!¨í èú ü1?0′?4?? × H·19è DNA#c?"?,XN2Eó ￥)èU? é ￥Zc?μCG￡,X=rè￠5àS ?o!¨EW ,Xc?DG￡ )?'ú&??rKS?ú$*ó(=:?hA1?A?èút-1c? àHtDBg,X?-1 c?Eˉ> !¨EWúd?4￡?aà?tYo,X-è0J!?? üE? ?,XY HG?è GSᤠ?Au1k,Xh*üèc?!¨EW,X ?/?1k"?3?4￡ ￥)kC^9C^E?Eóè3C^9C^?'sè?4￡6?ó C3Tc?DBgrKS,X!9???yè?à?4￡1Y?o?,X??(=/?,X ? ′4?,X<c?èE? 1Y?2O ? ′c?,X?oEW?,X ?è?à?4￡Eˉ9!¨EW ? ′4?ê·è3Aèèí?t(= /?Eˉ> < ? ′4?c?!¨EW?4￡áa?tz?? c?!¨í,XEˉê ?. 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PDF XCHANGE ww w.d ocu track.c om ?5?E-/?!_ ?è><aE-o!_ ?|9!5ùê￠ a5ù?,X?E-o 0N! üc?!¨í ê*üE24á,X-á4??>9?V ò 7.1è üc?!¨íè ￥)?Zt0N!? | S S *| Mouse IVGGYNCEENSVPYQVSLNS GYHFCGGSLINEQWVVSAGHCYK SRIQV Crayfish IVGGTDAVLGEFPYQLSFQETFLGFSFHFCGASIYNENYAITAGHCVYGDDYENPSGLQI * Mouse RLGEHNIEVLEGNEQFINAAKIIRHPQYDRKTLNNDIMLIKLSSRAVINARVSTISLPTA Crayfish VAGELDMSVNEGSEQTITVSKIILHENFDYDLLDNDISLLKLSGSLTFNNNVAPIALPAQ | S S | Mouse PPATGTKCLISGWGNTASSGADYPDELQCLDAPVLSQAKCEASYPG KITSNMFCVGFLE Crayfish GHTATGNVIVTGWG TTSEGGNTPDVLQKVTVPLVSDAECRDDYGADEIFDSMICAGVPE o *| S S | Mouse GGKDSCQGDSGGPVVCNG QLQGVVSWGDGCAQKNKPGVYTKVYNYVKWIKNTIAAN Crayfish GGKDSCQGDSGGPLAASDTGSTYLAGIVSWGYGCARPGYPGVYTEVSYHVDWIKANAV ò 7.1?±\!&?E? üs6?TUG??U?í5?T?,X6?<?,QGJ? Swiss Prot P07146? `?Um <R,X6?<?,QGJ? Swiss Prot P00765?0!¨íè,ì à,X!_ ?*ü??4???è ü!¨íT???,X Yt`.?K? 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Human ZCr MATGQKLMRAVRVFEFGGPEVLKLRSDIAVPIPKDHQVLIKVHACGVNPVETYIRSGTYS Ecoli QOR MATRIEFHKHGGPEVLQA VEFTPADPAENEIQVENKAIGINFIDTYIRSGLYP . . ******. . . * …. . . * *.* ..****** * Human ZCr RKPLLPYTPGSDVAGVIEAVGDNASAFKKGDRVFTSSTISGGYAEYALAADHTVYKLPEK Ecoli QOR PPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIIADKAAILPAA * ** *.. **.. ** . * **** . . * *. ** Human ZCr LDFKQGAAIGIPYFTAYRALIHSACVKAGESVLVHGASGGVGLAACQIARAYGLKILGTA Ecoli QOR ISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQWAKALGAKLIGTV . * * ** . * * * .. .* * * * *.***** *** *.* * *..** Human ZCr GTEEGQKIVLQNGAHEVFNHREVNYIDKIKKYVGEKGIDIIIEMLANVNLSKDLSLLSHG Ecoli QOR GTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVGRDTWERSLDCLQRR ** . . *. ** .* * ** …. * * * . .. . . . . * * . Human ZCr GRVIVVG SRGTIEINPROTMAKES SIIGVTLFSSTKEEFQQYAAALQAGMEIGWL Ecoli QOR GLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREELTEASNELFSLIASGVI * .. * * *.. . . . . . .*.** . . * . . * . Human ZCr KPVIGSQ YPLEKVAEAHENIIHGSGATGKMILLL Ecoli QOR KVDVAEQQKYPLKDAQRAHE ILESRATQGSSLLIP * . * *** *** *. . * .*. ò 7.2??G<!¨í?í?2O  J(?(=? Swiss Prot Q08257? ` E.coli8?G`!?êE? sGJ ? Swiss Prot P28304?,X!ü ?GLc?Eˉ> !¨í?E-?t+ CLUSTAL W/?c? Higgins et al., 1996?k,X?G<!¨í4§p? ü!¨í??èó ?></!_ ?,ì àè'&?></E-t!_ ? ±\,X? ?ó,Xc?!¨í?"? ?h*übFwo ü<KS8× èYKY1T ),ì?,X?oc?? <c?!¨í  íc?Eˉ> </??￡èEˉ> !¨EW?1TA|A?,X6?<?,QGJ `  J(?(=Kè,X!¨EW2b<c ?!¨í?KY1T ),X)×64§X 3,X<?,Q?8 ?1S*ü<c?!¨í,X1*+9è1Y,X à$dc ??t4￡E?rBüT,X ?ê NI-?è 3/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om <?,QBü,X? +?Bü A??<?,QBü ü</?8× èYJáKY,ì?è H"+??,X? +4§X 3?5à?? ò 7.3 ￡E?ZE- ,X?t_$èV ò/,X ü>±E?/?,X?/?<?,Q,X4??4§XèWà±> ′ $ XII? F12? `4?4? _>4x<?,Q$?GJ s#ê ′$? 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PDF XCHANGE ww w.d ocu track.c om c PLAU 90 EPKKVKDHCSKHSPCQKGGTCVNMP—SGPH CLCPQHLTGNHCQKEK CFE 137 PLAT 23 ELHQVPSNCD CLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTCYE 72 ò 7.5?&?L ?C?X ò `!¨í?YE-Y/??? òF?></?2O%?GJ>4x<?,Q$?GJ s#ê ′$ ? PLAU:Swiss Prot P00749? `4?4?>4x<?,Q$?GJ s#ê ′$? PLAT:Swiss Prot P00750?  à EGF,ì,X? +,X!¨í4§p? a) .Ht<?,QF?+ DOTTER/?cEˉ> !¨EW?E-G? ? /Z à EGF? +,ì,XEW?  3,X? ò× b)+ BLASTPk,X!¨í,XC?X ò× .c).*üBE?,X+0ú6? /,X BLASTP0N!!¨í? ?U)ú?·C?X òè?B5?t`4è$èNJ&?></c?!_ ?Kè,X&??a&?L ></!_ ?D ,ì ??è"?4?!?TE2y?tNJ&?,XE?4ìíh?tc?T G!,X!_ ?è"G `0a,è4?!?,XE?4ì íh?tc?1Y5à o?tc?T"uY,X!_ ?è6 1A±AèèE-oE?4ìG ?4??Z!¨í,X0N !è< òíhZY ?6?,X!¨í?NOu??,X e2?0NKè èE-t0NKè!￡5 ?6?,XC?XF?íh b?/?!¨í? ?G!¨í?"? L8Z¤o\áG??U,XK?Nlèíb??K?Nl5à??è!¨í?"??/?? è\Y??U￠?EY?? Q,X?tê′t?"?èE-^?/?!¨í￡E???tC?X,XVé??A??Au1k/￥:,XK? NlF? ?11TêE?E? ò><?"?ìC?X?!¨V?R￠4?4z'+ A±?G￥E,X?Y,XEè X??ZE-?,?,X?4￡.B0?ZA??> Y,X1k"?èí!￡?/?C?XF?Y??UíJEˉ> ¤/? ?T,X'úèE? í"?E-?EèX,X!￡?!9,XrG￡Eˉ> t `?è2??,X'ú/?cú ü?[ -E?è üE-G??à ?n,ì à!_ ?t!7úèY|9ê5,X!_ ?tBóú?7ú?è BE- ?nè? üE?,X!¨í?"??k?P?úè3?à?R,X?GC?X? ?y?à's],X Needleman Wunsch1k"?J\í?"?Gc?!¨íE-?K?NlA?Au,X|? ????ì1*+9? Needleman and Wunsch,1970??|????,X??E- ,XèVp?5C?X4? !6b?GC?XT,X?&?èFwE-5C?XDCK&?E-tKè&?,X?GC?Xè3Aèè ?)?t4?!6b?GC?XT,X?&?,X ?4{C?X?' 4?!6bE-?&?,X?GC?XD?E-  è?GC?X ?1E?E?^ ?t?G,X ?4{C?XE2y5à?? ü ?,X Needleman Wunsch@? ><E?è?G!¨í?' í!￡tc?F?+?7?4?èAè￠e2?0NKè,XoT?|,è7? ???|?6 1A±AèWe2?</?!¨í? ' 5àèíE-/? ?1*+9/á0?  ?1r)??G,XF?!¨í?E-/?!¨í,XC?XáL??UE?e 2? ò,Xè ?L??U üYF??? `4?4§?Vp¤/?!¨í,X'úá? ′rtê￡?!¨íKó ,XDG￡5àrtêèE-/?!¨í?G,X?E-tE?/?qC*b'ú2?43,X?BüèAè¤/?C?X ,X'ú? üá G!,Xc?!?!5B￡??1? -E?,X'ú2?43 ü"E-t????'úL!Lê êèC?X,Xê)ú?4?!6è?t?,XC?X?hE¤5à*ó?E- è?à?kA??(à0?,XC? XèWà1á G!,Xc?!?+ L$5àá￡ ü</?!¨í1c?,X4§0+ L$? üE-oC? 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Comparison of: A. f9 human.aa >f9 gi|119772|sp|P00740|FA9_HUMAN COAGULATION FA 461 aa B. f12 hum.aa>f12 gi|119763|sp|P00748|FA12_HUMAN COAGULATION 615 aa using protein matrix 7 35.4% identity in 254 aa overlap; score: 358 220 230 240 250 260 270 F9 QSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVE TGVKI .:....:::::: : .:. :. ..: ..::.::... :..:::::.. . .. F12 KSLSSMTRVVGGLVALRGAHPYIAALY WGHSFCAGSLIAPCWVLTAAHCLQDRPAPEDL 370 380 390 400 410 420 280 290 300 310 320 330 F9 TVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPL VLNSY ::: :... ... .. :. .: . :...... .:.::.::: :.: .:..: F12 TVVLGQERRNHSCEPCQTLAVRSYRLHEAFSPV SYQHDLALLRLQEDADGSCALLSPY 430 440 450 460 470 480 340 350 360 370 380 F9 VTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRS ALVLQYLRVPLVDRATCLRSTKF : :.:... . .. :.:::. :. . . : :: .::... . : .. F12 VQPVCLPSGAARPSETTLCQ—VAGWGHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHG 490 500 510 520 530 390 400 410 420 430 440 F9 TIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTS FLTGIISWGEECAMKGKYGIY .: .:.:::: ::: :.:::::::: : : .... : ::::::..:. ..: :.: F12 SSILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKPGVY NI-?è 6/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om 540 550 560 570 580 590 450 F9 TVVSRYVNWIKEKT :.:. :..::.:.: F12 TDVAYYLAWIREHT 600 610 8 34.7% identity in 49 aa overlap; score: 120 100 110 120 130 140 F9 VDGDQCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGR .....: .::::.::.: . . : :: :..: :..:.. . .:: F12 LASQACRTNPCLHGGRCLEVEGHRLCHCPVGYTGPFCDVDTKASCYDGR 180 190 200 210 220 9 33.3% identity in 36 aa overlap; score: 87 100 110 120 F9 DQCESN PCLNGGSCKDDINSYECWCPFGFECKNCE :.:... :: .::.: . .. .: :: ..:..:. F12 DHCSKHSPCQKGGTCVNMPSGPHCLCPQHLTGNHCQ 100 110 120 130 ò 7.6??G ` ?G,XF?!¨í? üS*ü LALIGNí?2O±> ′$ IX? F9;Swiss Prot 900740? `±> ′$ XII(F12;Swiss Prot P00748)Eˉ> !¨íê ￥)?ZYt?G,X!¨í4§p? a·ú `0N!?5. ?!￡E?,X'ú2?43??S*üb1T ),X G!á G!,X??è ü!¨EW<?,QBüêè?à ?1 *ü a·-?L 9r?!¨í,X#ó??\' è ü,ìG<?,QBüKèè¤o!ü ?GL ?1\?? ,ìf a·5àá*ü ?Wà,X*ó)ú*óê?BüèE-o±\ a·,X_$ùà??!üGL ? isoleucine? `N]!üGL? valin??'/??è+c"?è?!üGL? serine? `8￡!üGL NI-?è 7/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om ? threonin??U??? üAu1k!¨íúêè,ì à,X!ü ?GL'ú?P?b a·,X!ü ?GLè5à± \,X a·'úP?bM2±\ ?êè6 1A±AèèA?AuZ?2??,Xúè5àèè ü!¨íM2 ,ìE￥,X c?? mouse ` rat,X à$d ? ′?1 ???U?,Xc?? mouse ` yeast,X ? ′?ê?A?Au?á à2?43,Xúè5×<%E-o ′2?èS*ü a·-?L ?UYyè üE-t-?L è?)!ü ?GLG! í,Xú??,?Z' ? 1?tS"ˉS*ü,X?ì-?L ?0? üEˉê,X&?0U ?? _T? PAM?? Dayhoff et al.,1978?? ?t PAM?tEˉê,X ?? )! G 1%,X!ü ?GL ?èE-Já? G-4￡E? 100 ? 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L è7??Y 62%,X,ì à!¨_,Xc?>4? ü??tc?è ′!8 a·Ne)[èt ?Fwo!¨0N! ?ê E??,Xc?,XU?E ?è a·-?L ü?)úP?z,ìc?êS*üP?,XKü?,è7? BLOSUM90?è ?)ú???,Xc?êS*ü",XKü?,è7? BLOSUM30?? ò 7.8? BLOSUM62ú-?L ? Z>9SFwo|9ê5è ?1 ü!¨íé9?o0N!èá6?t?è úí?Sú$ ?kM6 ,?<M2?!￡é9?t?>?è!¨í,XúF??Y7L8èíbE-o?>?YA??5.ú,X??í??  *ü,X?t*ü?tLt,X5.ú!¨_ ?,0N!,XKSzèJY?t ?D?*?Yê/??>? ??5.? `/??>?ê ?5.?èíb?tKSzQ,X0N!è7úD G+Lnè üEY?0N ! ?Dêè ü\?/?zT 4￡P`,XèEY,Xú\??Y)úA?T,X??E? 9Aèèíb G ?EY??tP?ú? ü BLOSUM624z 10 15?èíb/?EY??t,ìí,X"ú??4z 1 2?èEY ?E-t8× è ′|9 ` ??\5)??,Xè'Wà?o ￥*óè?E ??2??LE￥,X!_ ?? NI-?è 8/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om !¨í,X43Au::+? í?)?t!¨Kóè?àF? ?1Au1k?túèG??U,XL??U?nE-tú úC?óP?è ú6?ó¤oEˉê à$d?,XA?B? ü?·?E-?K?NlêèíbJ' ?)?,X?P?úèYo??\Y }èè"uY?tD:)úA?6?ó￡E?</?!¨í,Xúú×èJ?t6?A?JG??U?,X ?"?úk,X!¨íú `Fwo à KSz `4??,XLcc?Eˉ> !¨EW? èíbF?!¨í5à??è???UQk??!7VK?Nl￠1T )??è?àOj"??Fwo "uY??0N!kF?!¨íèE-/?!¨í>/?P?ú(!?G!í? HSP?? HSPE? *ü Eˉk Smith waterman1k"?ê1T ) S*ü?,X0N!5.ú?"?9?k? Karlin Altschul43Au:￡E?Lc,X HSPú,XúפoZD:)úA?èV)[?z?D6?>/? Uú× èE-\k"??è ′è èBF!è?8,Xú×,Xh*ü ?6?? ?W,XG??U?è^?t?-1k!¨íú S àNXó,Xú×,ì G6( ?6??Au1k? Pè￠5à4-?E-tú,X!¨í:+?,X ?6???E? è3C^C_E￥bLêè úC^Y?? ,ìG,X ?G￡ E></úá"b6k ?6?,X!¨íDG￡è5àUú×+?t ?D></è G K `  è ? 1k?·d?·èJèíb?)'ú2?431 ?6?CNe)[F? ?n,X?!¨í,X:+?qC*be2? 0NKè,X???￡ ü9 úRJ\qC*b9 ú,X????e2?0NKè,X??+c?KSzAu1k? 9è+b43Au,X!7.B?èE-tKSz?NO+F?!¨í,XNXóKSzEˉ> ?!7è1!?)?E?4ì h? Altschul and Gish,1996?èL??UEˉ> E-/??!7E? ′ üe2?0NKèE?4ì??,X!¨í üE? ?tYú!?CY?c?,X8× è? ^!¨íL$b"uY0N!,X ?.TèSK?Nl??1Têè H7/?ú$*ó(=:,XrL??? rLTè?U?0??t|9 `5,X2?.B? _L??U0N!èVp0N!,ìíEW?è üE-o0N! Kè?' ?19?kP?ú  3èY·><?,X ?6??9?k2??,ìF?,X HSPè üE-/????è￠ 'T ?A?W,X:+?EW ü)ú,Xè3A?è!￡t(!?Ják\G??Uè′t(!? àê ?)?át￡J' _êZ? Karlin Altschult `43Au: ?1Au1k1t HSP,X43AuèE-t? "?,XrBü^1t?G(!?,XúEˉ> tè￠5àAu1k_êJ' ￥*ó,X ?6??èJW?oA?B 3>*ü9.BAxE-oú ? ü(!?a!¨í?7è,X???Eˉ> t?<Q' t,Xúú×a HSP ú??Y??è?' ?1k?·d?·? ? aè?' Y??UíF?fKó,X:+?Eˉ> ü)úA?èJùàZ? _,X0N!?!7V à? 43,X Smith waterman!¨íè<Q' "uYP`,XA?Bè?à?' AxE-o!¨í,Xú3hA1FI~ Uú×èèú× ?D. `  ,Xá6?E?E?Au1k9?kè'' èE?E?? _9?kE-o,X?"? ?4￡>??  ￥)Z? DBg,X,ì?e2? TE?A|A??UL?bFwoEW(M?,X G!,Xc?èíb?t? ￥)?,Xc?è?à′"?k -1*ü?c? àWEˉ> !¨íèDBg,ì?e2?S?à6?ó￠DBg, ü,XD ?tc? ?EY? ?6? àóHCw,Xc?YG6(,Xc?èE-t?"?Yê?D7è??á,X 9??*üE-/? 1*+99?k?s,X1?t_$?à ′!8 ￥)?+?!|6?+? ? ′ v sis4?624ê-?>?S#*ó*óKS ′$,X ? ′,X?t ?'6? (Doolittle et al., 1983; Waterfield et al., 1983)?Fwtê íèc?DBgE?á?è ′!8E-t ￥)?C?1 o?ó?ú??' 5à?yVpEˉ> DBge 2?Jè?′9?,XA±èFwè o?óC ?·Z?V àJW′t?,X(=/? ? ′4?? èGI!? saccharomyces cerevisiae,X ? ′4?<c??4￡>#n?9? ü6Ta|(=è?G￡,XF?ú ? ′AìV?2O `5?T?,X ? ′F??4￡>#nJ,9 ? ′g ? genebank?èE-3D7èZ ><E?c? ?1R ? EST?1/?? EST(!?,X?U*üEè üDBge2?è*ü EST(!?Eˉ> cDNALZ ?1ú/? ?óHCw,X ? ′èùàJW? _*ó(=,X à$d ? ′??E￥yD,X?G?Yú"?7N6?+?? MENI? ? ′ `?a5?T?,X?t EST(!?,ì G!èJ? ü MENI ￥><!?H?4￡9g±,Z (Chandrasekharappa et al., 1997)? NI-?è 9/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om üDBge2?è ??0ú 1A?c? `DBg,X Nlc? 0!¨í?!¨í4§pf? Q,X hit listè aM6?2??,X )(à,X!¨í??è1 ?á à,Xú `43Au?V ò 7.9??? [ú?Ao4??4?EY?á à,Xe2?/?c?c?DBg `á à,X ?DF??íe2?{*óE ?è5àè E?Yá à,X+ M6è!¨V?0 ? Q?? WWW6? ` E mail1? ò 7.104-?Z?tS*ü Web+ M6Eˉ > DBge2?,X_$?E-/?6?,X?tì&?í?)?tóHCw,X!¨íè<F?"??· `[) h*üF? ?1E?E?CY[1T )?? 6(y7? s?,Xc?5,? `,ìG,X ü4?[)? a The best score are: initn initl opt z sc E(59248) gi|1706794|sp|P49789|FHIT_HUMAN FRAGILE HISTIDINE 996 996 996 1350.4 0 gi|1703339|sp|P49776|APH1_SCHPO BIS(5’ NUCLEOSYL) 431 395 395 536.2 2.8e 23 gi|1723425|sp|P49775|YD15_YEAST HYPOTHETICAL 24.8 290 171 316 428.1 2.9e 17 gi|1724021|sp|Q11066|YHIT_MYCTU HYPOTHETICAL 20.0 178 178 184 250.7 2.2e 07 gi|417124|sp|Q04344|HIT_YEAST HIT1 PROTEIN (ORF U 159 104 157 216.2 1.8e 05 gi|418447|sp|P32084|YHIT_SYNP7 HYPOTHETICAL 12.4 139 139 140 195.0 0.00028 gi|1351828|sp|P47378|YHIT_MYCGE HYPOTHETICAL 15.6 132 132 133 183.9 0.0012 ?gi|1169826|sp|P43424|GAL7_RAT GALACTOSE 1 PHOSPHA 97 97 128 169.7 0.0072 gi|418446|sp|P32083|YHIT_MYCHR HYYPOTHETICAL 13.1 102 102 119 166.8 0.01 gi|1708543|sp|P49773|IPK1_HUMAN PROTEIN KINASE C 87 87 118 164.5 0.0014 gi|1724020|sp|P49774|YHIT_MYCLE HYPOTHETICAL 17.0 131 82 117 161.5 0.02 gi|1724019|sp|P53795|YHIT_CAEEL HYPOTHETICAL HIT 98 98 116 161.5 0.02 gi|1170581|sp|P16436|IPK1_BOVIN PROTEIN KINASE C 86 86 115 160.4 0.023 gi|1730188|sp|Q03249|GAL7_MOUSE GALACTOSE 1 PHOSP 87 87 120 159.3 0.027 gi|1177047|sp|P42856|ZB14_MAIZE 14 KD ZINC BIODIN 132 79 112 156.3 0.04 gi|1209081|sp|P07902|GAL7_HUMAN CALACTOSE 1 PHOSPH 78 78 117 154.8 0.048 gi|1177046|sp|P42855|ZB14_BRAJU 14 KD ZINC BINDIN 115 76 110 154.5 0.05 gi|140775|sp|P26724|YHIT_AZOBR HYPOTHETICAL 13.2 115 65 109 152.6 0.064 gi|1169852|sp|P31764|GAL7_HAEIN GALACTOSE 1 PHOSP 62 62 104 137.9 0.42 gi|113999|sp|P16550|APA1_YEAST 5’,5’’’ P 1,P 4 TE 108 66 103 137.1 0.47 b >>gi|1169826|sp|P43424|GAL7_RAT GALACTOSE 1 PHOSPHATE UR (379 aa) initn: 97 init1: 97 opt: 128 z score: 169.7 E(): 0.0072 Smith Waterman score: 128; 30.8% identity in 107 aa overlap NI-?è 10/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om 10 20 30 FHIT MSFRFG QHLIKPSVVFLKTELSFALVNRKPV ...: X.:.. . : .: ..:: : GAL7 VWASNFLPDIAQREERSQQTYHNQHGKPLLLEYGHQELLRKERLVLTSEYWIVLVPFWAV 190 200 210 220 230 240 40 50 60 70 80 FHIT VPGHVLVCPLRPVERFHDLRPDEVADLFQTTQRVGTVVEKHFHGTSLTFSM—QDGP : ..:. : : :.:. .: : : :: .: ... : .. X. ::. .:: . .: GAL7 WPFQTLLLPRRHVQRLPELTPAERDDLASTMKKLLTKYDNLFE TSFPYSMGWHGAPMGL 250 260 270 280 290 300 90 100 110 120 130 140 FHIT EAGQTVKH VHVHVLPRKAGDFHRNDSIYEELQKHDKEDFPASWRSEEEMAAEAAALRV ..: : : .:.: : GAL7 KTGATCDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRVLPEVHYC 310 320 330 340 350 360 ò 7.9?Eˉ> FASTAe2?,XEg???D?*ü?2O4?!üGLY6('<?,Q0? Swiss Prot P.49789? 1A?c?è1 SwissprotDBg ?.èEˉ> FASTAe2?k,X Q4§pè üE-t?0è ?D ktup=1× (b).1DBg,X?t5!? ü Q?><11????1A?c??Jù ?5?T?,X 1 .?GL G2a8?GLE@/?GJc??k,X?GF?!¨í4§p?<Q' üE-G?èc? ,X,ì?átQèE-o<?,Q ü4§XTF?/Z\Q,X,ì?? 7.10? ü WWWTEˉ> DBg,ì?e2?? NCBIDBge2?,XP?4{ BLAST6?è ü Web5%NIT?? r)??1A?c?hA1+>?S2lC??,X[è? ü òè/,X U43746 c???e2? oê?o ?,X2?ùàe2?/?c,X á+1 ?DBg,X á+èE-?t2?F? ?1E?E???EY??VpL??U,XA±è ?1A?nLt,XEYNM ?D?E-G?E?Y?t ?,X BLAST 6?è'' P?4{,XEYNM ?D>Ld;￡CK9Z?? aè1T ) &???? “Submit”Kè¤xA?" a ?1??e2?Z? V?,Xc?DBgM2 ?èJè!71'ú&??,XEózá?rKSè üE-/?5ê?èy*ü|?/? c,X?"?,èyEˉ> DBge2??4￡ ?ká?rL??t?·??"?S*ü? _Au1k `,ìG ,X(M!^.@êè?à?UA|A?,X,?,XBE?Au1k6?Fo??'?G?"?á ?> êè?à ?NO"}bFwo  ￥??"? èE-o?"?úy*üZE￥1t?c?!¨EWè àê? üJíE? !7.B!¨íE-??M6f?&?L=? NI-?è 11/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Y?/?  ￥??"??0? üE- ,X1*+9TèWúc?ú?·?+E24á+!?4??,X-á?/? +  ?? ?b+,X?"?è ü? H·?ó+ Wilbur ` Lipman¤?èJèS"ˉS*üb?y,Xe2? /?c?J ???E- ,Xè?t6?óá/?!7.B,Xc?G2?,X!¨í7??ù ??t?t c?F?1Y,X+è^1A?c?,XY+4ê?2?éèJè üDBg?￡1A?E-o2? éèE-o?,X+?\?>HHn?9? 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PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om ?L!èE-êèúáa ?6? ? 26?E-t ￥)?Lt,X  ￥?-1A?¤oZqBè ′!8è 'ú,XL!"?aFAF,X??,ì!¨?CYE? ú?L!Kü Xêè Q,Xê ?4?!6?bè 2?43 2￡?!?′??,X Qê è4?4áEˉ> JW?0? S*ü BLAST ?1E?E? e Mail? WWWê{ ? Q??0 BLAST/?cè′A?V)è? ?DBge2?ùà ˉ/? ? 2?? BLAST/?c,X á/?èDBg á/?è1A?c? `?G￡,X üE?,X ?Dè\' è'1T 2? ￥*ó ?êêèe2?,X4?8V?Lc ??ZFS!#?#?è?à^ BLASTs6??￡E?BE? á A?èFS!¤ ??Y1K?A?5ù ?6???U ?5×S*ü,X?Y1K,XYGY???UkGb*ü e Mail;> BLASTe2?,X?4?è4- blast@ncbi.nlm.nih.gov ￥?? ?Y “HELP”,XF?ê× ü WWW 1Kè} ü4?,X×VpS*ü Unix2?43èS*ü man blast ?19?kAo4?,X}μC? >< 7.1? BLAST/?c? ′/?á à,X BLAST ?1E?E?1A?c? `DBgc?,X2O _9t1 ú? blastp!¨EW,X1A?<? ,Q à<?,QBüDBg×,ìhb GLc?,X/?c blastn×Vpc?2O _á àè DNAc? ?1>5? A￥?<?,Qc??YA/?KùA??? a à<?,Qc?Eˉ> !¨EWè blastx!¨EW?t DNA,X1A?c? à?t<?,QBüc?gèJ4§píúd?c? ` ESTs\Y*ü×íb?t ?b GLc?g,X<?,Q Bü1A?è tblastn/?cíb?RDBgc?,X?,X4ê-? \Y*ü×? a?t ? ü(M!^??? 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Gss GenbankGSS(genome survey sequencesè ? ′4?#nc? ? Yeast GI!?,X< ? ′4?c?? Ecoli ?6t9?,X< ? ′4?c?? Mito 6Ta|(=4?2f',X< ? ′4?c?? Alu eL?Z&IKS2O|(=,X AluG?ác?? vector eL?Z#> ,Xú9?',XLZ? NI-?è 14/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om ,X?A? `￡E??1u##??ên>4y-áZè?à?' ?1,?5?T? `p=,Xc?F?>ù ?Eˉ 9Z????4-?Zk?G HSP? GSJWKùA??5?A￥4§p3?E? Q?,XKùA??? aM6Y?4-?Z?G HSP,Xú?S ` ?SAu1kêS*ü,X HSPD,?? ù ??/?p= EST?+1????,X!¨í ü ò 7.11bk1/?Jù ?Z?t HSPèJè /Z!￡?t,Xúè EST,XVé?5?A￥ à1A?c?Jf/?,ì à,X!ü ?GL!_ ? ü?tc? Kè 2è></?tá à!_ ? G!,Xú!7D?!¨V±\ a·??￠á àKùA??k,X? t HSP:+,XJèP!8,ìF?èE-?&?￠c? $? ?1,??9?E-/?6?></ ESTc?,X? /?KùA??JíA?,XèJèíb*ü,ìí?Jí?,X1KEˉ> c? ) ?E?> DBúdêUY ? a sum Reading High Probability Y sequence producing High scoring Segment Pairs: Frame Score P?1? N gb|AA056325|AA056325 zf53a03.sl Soarea retina N2b4HR H... +3 724 3.4e 102 2 gb|T03796|T03796 IBIB913 Infant brain,Bento Soares...+3 567 2.6e 78 2 gb|AA260597|AA260597 mx76g09.r1 Soares mouse NML Mus m...+2 239 4.9e 53 4 gb|H86456|H86456 yt01b06.s1 Homo sapiens cDNA clon...+2 323 4.3e 52 4 gb|N24576|N24576 yx72a04.s1 Homo sapiens cDNA clon...+1 365 5.5e 47 2 gb|AA265273|AA265273 mx91c12.r1 Soares mouse NML Mus m...+2 239 6.4e 41 2 gb|AA237206|AA237206 mx18e01.r1 Soares mouse NML Mus m...+3 159 1.5e 40 3 gb|R146001|R146001 yf34b10.r1 Homo sapiens cDNA clon...+1 278 1.5e 40 2 gb|AA200706|AA200706 mu03f12.r1 Soares mouse 3NbMs Mus...+1 343 1.9e 40 1 gb|AA045064|AA045064 zk77f12.s1 Soares pregnant ulerus... 3 269 2.3e 37 2 gb|AA087434|AA087434 mm28a04.r1 Stratagene mouse skin....+3 322 3.6e 37 1 gb|R05907|R05907 ye93h02.r1 Homo sapiens cDNA clon...+3 252 7.7e 37 2 gb|AA268820|AA268820 vb01c10.r1 Soares mouse NML Mus m...+2 234 7.7e 35 2 gb|AA162310|AA162310 mn44a07.r1 Beddington mouse embry...+1 134 8.3e 34 3 gb|N27820|N27820 yx54h10.r1 Homo sapiens cDNA clon...+3 154 7.8e 29 2 gb|AA234907|AA234907 zs38f03.r1 Soares NhHMPu S1 Homo... +2 155 1.8e 28 2 gb|AA231081|AA231081 mw11d11.r1 Soares mouse 3NME12 5... +3 134 8.8e 23 2 gb|H91652|H91652 ys80c04.s1 Homo sapiens cDNA clon... 3 215 3.7e 22 1 gb|H50532|H50532 yo30h08.s1 Homo sapiens cDNA clon... 2 211 1.2e 21 1 gb|AA150236|AA150236 zl03c01.r1 Soares pregnant uterus...+1 159 5.0e 21 2 NI-?è 15/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! 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PDF XCHANGE ww w.d ocu track.c om gb|AA144382|AA144382 mr15d12.r1 Soares mouse 3NbMS Mus...+3 159 7.6e 21 2 ?gb|AA390557|AA390557 LD09473.5prime LD Drosophila Embr...+3 130 1.6e 20 2 gb|AA210480|AA210480 mo86b03.r1 Beddington mouse embry...+2 128 2.0e 20 3 gb|H19021|H19021 ym44b02.r1 Homo sapeins cDNA clon...+2 134 5.9e 20 2 gb|AA283084|AA283084 zt14g09.s1 Soares NbHTGBC Homo sa... 3 175 2.3e 19 2 gb|H25759|H25795 y149d01.s1 Homo sapiens cDNA clon... 2 185 5.0e 18 1 gb|H33787|H33787 EST110123 Rattus sp.cDNA 5’ end..... +1 137 6.7e 17 2 gb|AA201988|AA201988 LD05058.5prime LD Drosophila Embr...+3 175 5.5e 15 1 gb|AA263526|AA263526 LD06652.5prime LD Drosophila Embr...+1 167 7.0e 14 1 gb|R46340|R46340 yj52c04.sl Homo sapiens cDNA clon... 1 151 5.6e 13 1 gb|AA246675|AA246675 LD05588.5prime LD Drosophila Embr...+2 117 2.8e 10 2 gb|AA282899|AA282899 zt14g09.r1 Soares NbHTGBC Homo sa...+3 118 6.1e 07 1 gb|AA247705|AA247705 csh0941.seq.F Human fetal heart,....+3 56 0.0039 2 b gb|AA390557|AA390557 LD09473.5prime LD Drosophila Embryo Drosophila melanogaster cDNA clone LD09473 5’ Length – 659 Score – 130 (60.4 bits), Expect – 1.6e 20, Sum P(2) – 1.6e 20 Identities – 25/60 (41%), Positives – 40/60 (66%), Frame +3 Query: 105 TIKSVRFYTEKNGQLIYTTFTEDTPSVGQRLLNSVLNTLIMISVIVVMTIFLVVLYKYRC 164 +I S+ FY + L+YT F E +P + +++ ++LI++SV+VVMT L+VLYK RC sbjct: 480 SINSISFYNSTDVYLLYTPFHEQSPEPSVKFWSALGSSLILMSVVVVMTFLLIVLYKKRC 659 Score – 117 (54.3 bits), Expect – 1.6e 20, Sum P(2) – 1.6e 20 Identities –23/30 (76%), Positives – 27/30 (90%), Frame +1 Query: 75 LEEELTLKYGAKHVIMLFVPVTLCMIVVVA 104 +EEE LKYGA+HVI LFVPV+LCM+VVVA sbjct: 391 MEEEQGLKYGAQHVIKLFVPVSLCMLVVVA 480 ò 7.11?? ? TBLASTNe2?,XEg?? üE- ? TBLASTNe2?è1 dbestDBg ?.è1Lè8ü NI-?è 16/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om #KT?!?+?? GEˉ> ?5?H?+è ? ? ′? Genbank ?2? ?-? L43964?,X<?,QBü{(=1A?c ?è,?,XZ￠JWFwo ?6? à?2O ? ′Y à$d?,X(=/?HHn??o cDNALZ??D? . Q?><,X?F?ú/ZJ?Q,X 25t Q?!￡t?2??9,Xc?F?+Wà,X GenBank?2? ?-?1 ??F?ún> 4???JùàZWà,XKùA?? `?G HSPúè àê/,XE?Y? tJ' Q,X ?6??,Xt `?? a??,XDB4-?Z üAu1kt `,X ?6??ê#] ?,X HSP ,XDG￡? üE-t Q?>< ?1??7?? 105￠5?T?k,Xc? `?5￠p=k,Xc ?× (b). àp=,X ESTc?? GenBank AA390557?)úA?T,X5?A￥c? G!,X4§p?RZ? t HSPsè!￡?tS*üá à,XKùA???,ì à,X!_ ? ü?> c?Kè,X,ìh!5B 2è5à “+” 0ú ??A?-Fwoá,ì àJ a·ú!7ú,X!_ ?? BLAST,X?? Eˉ ?E￥ ￥×,X BLAST/?c,X?Av(¤P?Ze2?Eóz?#óz `r*ü??E-t`<G??mE?,XECê ù?n 2.0(?FS! à WU BLUST#?#?èE-tECê+ ",ˉNS?:A?Au,XèYê/? BLAST2??hA1"??è ü ￥×,X 2.0(è Q?> ,X ?DY\? ?èJ?o *ü,X ? D? ü>< 7.4? ?t Eˉ97?bé ￥?t+ Q,Xê ,X???)? üè ü?tL??U5×<%,X!_ ?,X0k ·G?? NOR?t+ Q?S*üE-/?1*+9¤P?Ze2?Eózè ′?G￡Lc,X+ Qú?>? +9èJè\Y ?6?k?t:+?8CQ,X!¨í?1`t Eˉ6?óa.B 5àá ?:? ?)ú 0N!?L8Z}S*ü5ùèt?? )ú?·{*ó,X!¨íè?(E?¤P?ZEWE°G2?,X#ó?èJ  ?6??ù ?A??|9 `5?!¨EW￠?R′0N!,X HSPE-???1*+9??è' aèE-?!¨í 9?k?P?ú  3,X???>HHn?9èy-è￠E-?&? ?! ` ? aê èE?E?C,XC? XEˉ> ′0N!F?!¨í,Xe2??V à??,X HSPe2?è?tú?L!,XKü;ú??S×Fw oFAF?G￡Bó,X a·ú,XC?X?í=-,X HSPEˉ> ?á,XE-/??0èú?á/ oê,X ?0N! ,X!¨íèJ±A?Wà à?4￡y ,XF?úá?,ìx?E-t2?43á àb FASTAG? a,X1*+9è FASTA ??{*ó?t?G,X!¨í? >< 7.4??oíb BLAST\Y*ü,X ?D? ?D á/? BLAST 1.4 BLAST 2.0 DBg (database) 1? ?D d database 1A?c?[ê (query sequence file) 1` ?D I filename ó?Kü E (expectation cutoff) E = number e number HSPúKü S (HSP score cutoff) S = number s number +úKü T (word score cutoff) T = number f number ? Q0k · A (multihit window) n/a A number 'ú-?L (score matrix) matrix matrix M matrix "ázE?$? (low complexity filtering) filter seg F NI-?è 17/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om íbFwo?,X:+?EW,X!¨íèEˉ> EWP?#ó?,XDBge2?,X?t?"?S*ü AìV profile ><EB§ ,XDB4§X? Gonzalez et al., 1994??E-t1*+9 ?6?ò4￡>Ax tEˉ> DBge2?,X!¨EWEˉ,XAòNlè BLAST,X?t?(M?1TêZ ?b profile,Xe2? 10??t profile ?6??>)ú?·?t?><èJ??Z ü?t±\,X<?,QBü4§X 3!￡? t!&? ￥)?!￡?/?!ü ?GL!_ ?,XNe)[??0??t profile ?6?\# G,XèJμC￠Fwo1 Y?àóHCw,X<?,QBü4§X 3,X?c?!¨ík,XèE-o!¨í?NONX??Qè5àèè ü E-G?YA??TT,XK?NlE?"uY?·?? !&?(M? ?á BLAST? PSI BLAST?? BLAST2.0,X?t(M?èJ?t profile>á?4?4?J èá?2?4??E-tE?/???bS*ü?t1T )1A?c?,X?t??,XDBge2?? üE-t?? ,Xe2?4§pè?t profile￠P?z:+,X!¨í9?kè' aE-t profile ü1`EB,XDBg e2?S*ü?VpL??U,XA±èE-tE?/?? ?áEˉ> èJè ü?0Z2?4? profileè? ü!￡ ?EBt9?,Xc?? Z$è/ PSI BLAST?"?,XP?#ó?èo!üGLY6('<?,Q? HIT?c?>*ü90DBge2? ,X1A?c?? HIT ` 1 .?GLG2a8?GLE@/?GJ? GalT? ?b!&?G? ′,XY!4§X,ì??E￥ k￡E? (Holm and Sander, 1997)?4￡E?? ???,X??EB? BLASTPe2?è"uY ￥)??tí GalTc?Y:+,X Q?4￡E?? ?e2?è ü!￡? ? ?áF? ￥)??,XG2?è!7V ò 7.12 /? ü1` ?e2?Z ￥)?5?T?,X GalT<?,QBüèJè üE-?μC>t9 profile aè oê ?oJW(=/?,X à$d(=3>?#?9? Sequences producing significant alignments: Hign E Score Value Pass1: sp|P49789|FHIT_HUMAN FRAGILE HISTIDINE TRIAD PROTEIN 290 7e 79 sp|P49776|APH1_SCHPO BIS(5’ – NUCLEOSYL) – TETRAPHOSPHATASE (ASYMME... 117 8e 27 sp|P49775|YD15_YEAST HYPOTHETICAL 24.8 KD HIT – LIKE PROTEIN 88.0 6e 18 sp|Q11066|YHIT_MYCTU HYPOTHETICAL 20.0 KD HIT – LIKE PROTEIN 52.7 3e 07 sp|Q04344|HIT_YEAST HIT1 PROTEIN (ORF U) 45.3 4e 05 Pass2: sp|P47378|YHIT_MYCGE HYPOTHETICAL 15.6 KD HIT – LIKE PROTEIN 70.5 1e 12 sp|P32083|YHIT_MYCHR HYPOTHETICAL 13.1 KD HIT – LIKE PROTEIN IN P... 59.0 3e 09 sp|P26724|YHIT_AZOBR HYPOTHETICAL 13.2 KD HIT – LIKE PROTEIN IN H... 57.6 9e 09 0N!?5.ú (gap opening penalty) n/a G number 0N!§)5.ú (gap extension penalty) n/a E number PSI BLAST ?á (PSI BLAST iterations) n/a j number NI-?è 18/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om sp|P32084|YHIT_SYNP7 HYPOTHETICAL 12.4 KD HIT – LIKE PROTEIN IN P... 55.7 3e 08 sp|P53795|YHIT_CAEEL HYPOTHETICAL HIT – LIKE PROTEIN F21C3.3 54.3 9e 08 sp|P42856|ZB14_MAIZE 14 KD ZINC – BINDING PROTEIN (PROTEIN KINASE... 52.8 2e 07 sp|P42855|ZB14_BRAJU 14 KD ZINC – BINDING PROTEIN (PROTEIN KINASE... 50.2 1e 06 sp|P49774|YHIT_MYCLE HYPOTHETICAL 17.0 KD PROTEIN HIT – LIKE PROT... 49.5 2e 06 sp|P49773|IPK1_HUMAN PROTEIN KINASE C INHIBITOR 1 (PKCI – 1) 49.1 3e 06 sp|P16436|IPK1_BOVIN PROTEIN KINASE C INHIBITOR 1 (PKCI – 1) (17 ... 48.7 4e 06 sp|P44956|YCFF_HAEIN HYPOTHETICAL HIT – LIKE PROTEIN HI0961 47.3 1e 05 sp|P43424|GAL7_RAT GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 41.0 8e 04 Pass3: sp|Q03249|GAL7_MOUSE GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 87.2 1e 17 sp|P07902|GAL7_HUMAN GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 79.8 2e 15 sp|P31764|GAL7_HAEIN GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 64.7 6e 11 sp|P09148|GAL7_ECOLI GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 62.5 3e 10 sp|P22714|GAL7_SALTY GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 58.1 6e 09 sp|P09580|GAL7_KLULA GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 48.5 4e 06 sp|P08431|GAL7_YEAST GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 40.8 0.001 Pass4: sp|P40908|GAL7_CRYNE GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 71.0 8e 13 sp|P13212|GAL7_STRLI GALACTOSE – 1 – PHOSPHATE URIDYLYLTRANSFERASE 57.0 1e 08 ò 7.12?S*ü PSI BLAST aè#ó?¤P?\?? üE- ? BLASTPe2?è1A?c??2O4?!üGL Y6('? HIT?<?,Q? Swiss Prot P49789?èe2?ê? Z PSI BLASTs6?? ü!￡? ?G?áe 2?è??2??9,XKY43Au::+?,X G!F??/Wà,Xn> è'ú1 ? E D? "áz  3 á1u<?,QE? GLF?ù ??o#N[,X  3è üEˉ> c?DBge2?êE-o  3 ?6??D7è ?o??E?￥,X4§p?E-o "áz  3? LCRs? ü￠a,X à?6. üNN `-á <óG?á è2?4?,X???VJ¤oê?o!_ ?E??><)??,X8× èY ?ê??t/? SEG,X/?c ￥)CK 9è,?,X?U^?t<?,QBüc?ú?·"áz `P?áz4??,X ?t(!?? Wootton and Federhen, 1993, 1996??E-t/?c,X4§p><aDBg,X<?,QBüY? 1T1Y7???t LCR(Wootton and Federhen, 1993; Wootton, 1994)? LCRs,XEˉê?s6? `4§X?BüJ"uY >\Q Z?·? ü DNAèYA??/?1T ),XG?áèJ?o?4￡-1F'P?z? ?,XèJè ü 0 ? ′ òBê4￡ S*ü,X?Wà$dCK,X ?6?6. üGJ$￥|?#N[ 8?GL a·ê5ùá1x 6? LCRsè#Qb ü4§XT1M2)×6  3,X6?, üèFwo ü(=)úê:T?4￡>nM2)× NI-?è 19/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om 6,X  3E? ?1 üS*ü SEG/?cê9?kEWQ,X4§p (Wootton, 1994)? íbù ? LCR,Xc?Eˉ> !¨í?K?Nl,Xè ′E-oc?á0ú ü!_ ?!_ ?c?\&,X? _? Yoêíèas6?,ìG,X2? ?6??? <ó?ê4??4§Xè5àá?)(M?,Xc??5à èèí!¨í043Au::+?úd,X?"??0? ü?n,XLcVé ?.T,Xè LCR' á0ú üE- ?5êè ′!8èíb?tù ? LCR,X1A?c?è üEˉ> DBge2?,XEg?G?? ￥)?\?á!7.B ,X5,?è ′E-o G!,X:+?>E?P?A??Z (Altschul et al., 1994)?E-tK?Nl?'T ? 1E?E?E?$??ê5ù ?#;??·?è?0E- ,Xè^YK?Nl,X$c?E@êáa.B,X+0ú ?<?,QBü*ü;è GLc?*ü1?èE- Wàá?í!¨íB?)!7úZ? p=R2+ ? ′{(=,X?2O à$d(=ù ? LCR<?,QBü,X?tQ_$è ü*ü SEGúd,Xêíè? t"4??áz,Xc?  3>HHn?9? ò 7.13a/Z5,?,X?Eg?èJ"ázc?*ü ?m+!?></ üoE?èP?ázc? ü ?E?*ü?m+!?></?1?t  3(!?Y 61t!_ ?è ù ??G￡í!üGL? alanine? `B !üGL,¤? glutamine?,X?6.(=×1`t  3(!?Y 14t!_ ?è# ?b2?!üGL? arginine??VpáEˉ> E?$?,XA±èA??ù ?E-/?# ??c?,XDBg c?F??>y ?9?S*ü Q?> EYNMè SEG/?c?{*ó?tE?$? a,X1A?c?(? o êèE?$? ?1Y BLAST/?c7?|`?èVpS*ü üE?,X ?D?A?"?? üS*ü BLASTêè5,?? ?? ?1r> E?$??!¨V ü WWW(??E-?·G?Z?1A?c?,Xáa.B,X+0ú ? ü sc?"uY?)??? ü!¨í>J' ￥)?? a >gi|1703441|sp|P50553|ASH1_HUMAN ACHAETE – SCUTE HOMOLOG 1 1 11 MESSAKMESGG agqqpqpqpqqpflppaacffataaaaaaa 12 72 aaaaaaqsaqqqqqqqqqqqqqqapqlrpa a 1. DGQPSGGGHKSAPKQVKRQRSSSPELMRCK RRLNFSGFGYSLPQQQP aavarrnerernrv 120 133 1. KLVNLGFATLREHVPNGAANKKMSKVETLR SAVEYIRALQQLLDEHDAVSAAFQAGVLSP TISPNYSNDLNSMAGSPVSSYSSDEGSYDP LSPEEQELLDFTBWF b >gi|1703441|sp|P50553|ASH1_HUMAN ACHAETE – SCUTE HOMOLOG 1 MESSAKMESGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX NI-?è 20/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om XXXXXXXXXXXXDGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPX XXXXXXXXXXXXXKLVNLGFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHD AVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTBWF c >gi|540240 (U14590) achaete – scute homolog b [ Danio rerio ] Length – 195 Score – 193 bits (512), Expect – 7e 49 Identities – 107/155 (69%), Positives – 118/155 (76%) Gaps – 8/155 (5%) QUERY 86 KQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPXXXXXXXXXXXXXXKLVNLGFATLRE 145 K +KRQRSSSPEL+RCKRRL F+G GY++PQQQP K VN+GF TLR+ 540240 32 KVLKRQRSSSPELLRCKRRLTFNGLGYTIPQQQPMAVARRNERERNRVKQVNMGFQTLRQ 91 QUERY 146 HVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNS 205 HVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSA Q GV SP++S YS 540240 92 HVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAVLQCGVPSPSVSNAYS 146 QUERY 206 MAG—SPVSSYSSDEGSYDPLSPEEQELLDFTNWF 238 AG SP S+YSSDEGSY+ LS EEQELLDFT WF 540240 147 AGPESPHSAYSSDEGSYEHLSSEEQELLDFTTWF 180 ò 7.13?S*ü SEG/?c?2?"áz  3?S*ü SEG/?cí?2O achaete scute<?,Q? Swiss Prot P50553?Eˉ> údè ￥)?Z?!?"áz  3? (a).15,?,X “tree”?;> /?ck ,XEg?4§pèoE?*ü?m+!?/Z"áz  3è ?E?*ü?m+!?/ZP?áz  3? (b) .?  x Q?> ?Gè SEG/?cú?{*ó^"áz  3#;],Xc?4§p? (c). Z??S*üè?05ù ?1S*ü BLAST/?c9Eˉ> "áz  3,X#;?'?t"áz  3 >#;],Xc?01A?c?>¤x4-DBgEˉ> ?2?êè ü BLASTPEg?4§p,X!¨í ?6? 3?ùà?o>#;,Xú!?c?? G?áê Vp1A?ùà?tG?áê,Xc?!¨VAè?t AluG?á ?6???)?A??JíA?,X `??C ?· ,X4§p?<Q' ü<?,QBü<?,QBüe2?èE-?8á????K?Nlè üù ? DNAc?? )!¨EWèF??NOí!8éCK??U,XG???? ? ′4?c? ?6??ù ??G￡ú7,XG?ác?è(M? ?o? ? ′￡?_V Alus, LINEs `?,Xc?,X MERs?è*?7? mRNAc?3 ?6? ?YG?á c?è′"F?μC,XM25?A￥ ? ′!8èG?áê üDBgc?M2 BF!èVp1A?c? 3YE-oG?áè? ü!¨í?)??G￡á!7.B,X!7ú?<Q' G?áê/Z?G￡á à? NI-?è 21/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om úè?' YC?ó,X,ì?S!¨íKY?n,XP?:+??<Q' !¨í?C?C^E-oG?á5àá{M6 ,X )?c?è,èy￠DBge2?,XEg?4§p??3èE-Já5à???,X? GenBank ` Swiss ProtDBgF?ù ??ojc?? warming sequence?èE-oDB ?S *ü5ù??1A?ù ?G?ác?? Claverie and Makalowski, 1993?? ü GenBankèE-o5 ,?></Z?2O AluG?á,Xá àn/￥,X?7èc?× ü Swiss Prot,X2O5,? Aluc?,XA/? 5?A￥???ty-?tèKè+81F;úLh?? ü?/????è )A? “WARNING” ün> M2 :+?jc?á??)? ü Q?><,XT?è5àèè ?1YA??ù ? AluG?á,XDBgc ? à1A?c?M2 ,ìè*?7?!¨1A?c? àjc?E?,ì?E- ü ò 7.14aY')?èW/ Zí?2OE@) ′$ CBFB? ü 3’UTRù ??t Alu? ?b nrDBgEˉ> ? ? blastne2?,X?F?ú Q?jc??*ü1????!b Q?><,X1 31!?<Q' ?><NJF?,X?o G!/Z,ó!7 ,XG2??1?t?t7? Q?è4±??D ? ′KY AluG?á!??)?JíA?,X!7ú? ü1A?è,èy ?# AluG?á ú, ü,X?"? ü1A?!í aluDBg.? ?e2??V ò 7.14b/è.`E-t1 aèù ? alu,Xjc?0?P?ú G!>y ?9?Vp1A?c? > ￥)?ù ?G?áêèy?9,X> |?UíE-tc?Eˉ> 4êEe |è^W(L8ê5ù#; ]? üE-G??tY*ü,X1K CENSORèW6?ó7?|?#Jè#\L8G?áê? a Smallest Sum High Probability Y Sequences producing High – scoring Segment Pairs: Score P?1? N gb|L20298|HUMCBFB Homo sapiens transcription factor... 8691 0.0 2 dbj|D14571|MUSPEBP2B2 Mouse mRNA for PEBP2B2 protein, co.. 2574 0.0 25 gb|L032791|MUSP215CBF Mus musculus core – binding factor m 2574 0.0 25 dbj|D14572|MUSPEBP281 Mouse mRNA for PEBP2B1 protein, co.. 2130 0.0 26 dbj|d14570|muspebp283 Mouse mRNA for PEBP2B4 protein, co.. 1701 0.0 26 gb|L03305|MUSCBFAA Mus musculus core – binding factor m 942 0.0 27 gb|L03306|MUSCBFAB Mus musculus core – binding factor m 2130 1.6e 282 10 gb|U22177|DMU22177 Drosophila melanogaster Big brothe... 382 1.5e 37 2 emb|Y10196|HSPEX H.sapins PEX gene 400 4.4e 22 1 gb|L77570|HMUDGCRCEN Homo sapiens DiGeorge syndrome cri... 409 6.7e 22 2 gb|AD00067|1010603 Homo sapiens DNA from chromosome 1... 392 2.0e 21 1 emb|Z83822|HS306D1 Human DNA sequence from PAC 306D1 ... 392 2.0e 21 1 emb|Z82097|HSF77D12 Human DNA sequence from fosmid F77... 391 2.5e 21 1 dbj|D42052|HUMKIAA000 Human cosmid Q7A10 (D21S246) inser... 391 2.5e 21 1 gb|U83511|HSUB3511 Human Xp22 cosmids U177G4,U152H5, ... 386 6.5e 21 1 NI-?è 22/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! 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PDF XCHANGE ww w.d ocu track.c om gb|U52112|HSU52112 Human Xq28 genomic DNA in the regi... 386 6.5e 21 1 gb|S83170|S83170 tissue – type plasminogen activator.. 382 1.1e 20 1 emb|X9642|HSCAMF3X1 H.sapiens Y chromosome cosmid CAMF... 383 1.1e 20 1 gb|U95739|HSU95739 Human chromosome 16p11.2 – p12 BAC c. 383 1.1e 20 1 gb|95743|HSU95743 Human chromosome 16p13.1 BAC clone... 383 1.1e 20 1 gb|U91322|HSU91322 Human chromosome 16p3 BAC clone C.... 383 1.1e 20 1 gb|U82609|HSU82609 Human centromere – specific histone.. 382 1.3e 20 1 gb|AC001061|HSAC001061 Homo sapiens (subclone 2_g6 fromP.... 382 1.3e 20 1 emb|Z46940|HSPRMTNP2 H.sapiens PRM1 gene, PRM2 gene and... 382 1.4e 20 1 gb|K03021|HUMTPA Human tissue plasminogen activator... 382 1.4e 20 1 gb|U15422|HSU15422 Human protamine 1 (PRM1), protamin... 382 1.4e 20 1 gb|U91323|HSU91323 Human chromosome 16p13 BAC clone C... 382 1.4e 20 1 emb|Z54147|HSLI29H7A Human DNA sequence from cosmid L12... 381 1.7e 20 1 emb|Z82194|HSJ272J12 Human DNA sequence fom clone J272J12 374 1.7e 20 2 dbj|D0035|HIV2CAM2 Human immunodeficiency virus type ... 380 2.0e 20 1 ?gb|U14567|HSU14567 ***ALU WARNING: Human Alu_J subfam... 373 2.4e 20 1 gb|L81578|HSL81578 Homo sapiens (subclone 2_b2 from P... 386 3.0e 20 2 gb|L81854|HSL81854 Homo sapiens (subclone 2_b8 from P... 377 3.4e 20 1 b Smallest Sum High Probability Y Sequences producing High – scoring Segment Pairs: Score P?1? N ?lcl|HSU14567 ***ALU WARNING: Human Alu – J subfamil... 373 4.1e 24 1 lcl|unknown gb|M94643_HSAL001949 349 1.4e 22 1 lcl|HSU14574 ***ALU WARNING: Human Alu – Sx subfami... 347 7.0e 22 1 lcl|HSU14573 ***ALU WARNING: Human Alu – Sq subfami... 347 7.0e 22 1 lcl|unknown gb|Z15026_HSAL001005 (Alu – J) 324 1.4e 21 1 lcl|unknown gb|M15657_HSAL001254 (Alu – J) 337 6.3e 21 1 NI-?è 23/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om lcl|unknown gb|M61839_HSAL002304 (Alu – J) 314 6.6e 21 1 lcl|unknown gb|X17354_HSAL000525 (Alu – J) 329 6.6e 21 1 lcl|HSU14572 ***ALU WARNING: Human Alu – Sp subfami... 329 2.4e 20 1 lcl|unknown gb|J03619_HSAL001939 (Alu – Sx) 329 2.8e 20 1 lcl|unknown gb|L11910_HSAL002838 (Alu – J) 307 2.8e 20 1 lcl|unknown gb|M11228_HSAL002744 (Alu – Sp) 329 2.9e 20 1 lcl|unknown gb|L18035_HSAL004322 (Alu – J) 318 9.3e 20 1 lcl|unknown gb|L05367_HSAL002551 (Alu – J) 318 1.0e 19 1 lcl|unknown gb|M58600_HSAL002004 (Alu – J) 322 1.2e 19 1 lcl|unknown gb|Z23796_HSAL005276 (Alu – J) 306 1.7e 19 1 lcl|unknown gb|M90058_HSAL002955 (Alu – J) 294 2.5e 19 1 lcl|unknown gb|D14642_HSAL003786 (Alu – J) 315 4.0e 19 1 lcl|unknown gb|M29038_HSAL002942 (Alu – J) 314 5.5e 19 1 lcl|unknown gb|M92357_HSAL001387 (Alu – J) 310 9.8e 19 1 ò 7.14? ?áê ?6??D7è??E?￥,X4§p? ? blastn1A?S*ü,X1A?c??2OE@) ′$ CBFB(GenBank L20298),X cDNAc?? (a).VpS*ü nrDBgè?,X?o G! à1A?c ?KY,ó!7,XG6(è3?y A??á!7.B,X Q4§pèE-o Qú×b ?t?2O§8F' ,X ? ′4?  3? üE-t Q?><è'1???!b1 31> ?,X?7è,X Alu Jc?>?A:  c?? (b).VpS*ü aluDBgè Alu JA: c??Z?G G!c?? ZHHnE-o%0 ü,Xe2??pè:?a A?e2?4§pM2 G??U?TE?,X?o1*+9 ?h*üb Alu ?áèW?2O1 ?JW?o(=/??)?Ne)[?P?,XèJW?o ?á?' , üè<Q' ?G￡EW"è5àèèJW(=/??/?`<á à2O _,X ?áê?)? üY?tDBge2?Eg? ,XLt?BüèW ?1?/? ?áê?_Vè"??!¨ía DNAc?4ê-?  3,ìG,X!&?M2  Y,?,X?VpM24ê-?  3 G!5à4ê-?  3á G!èFw ?ác?\???+e×Vp1A? c? à?G￡c? G!èE-oc?,ìfKè"uY?G2?è!¨í,XúF?\,ìE￥èE-  ,X4§pU ?+e?_V ò 7.14aèA?? G!,X,ì?úF?′"? è5àèùàZ￠81 Fá à,X?2O§8F'T9,XBü2f?<Q' íE-t ￥)?Y\??·G?è?taN,X,?"?7? ?SAxE-t)?B5 ?6??bê+ ′2??V ?áê,X, ü?,XE ?? ?4§ üê+ ? /￥:?à!￡yF??U;> c?!¨í `DBge2?? T?!8èJèY,Xú$*ó(= :F?hA1's]E-o?U2?,X1K?E-o?"?"?n?Uá? ￥)èJèy ?á?rKS,XDBg?G￡ ,X?ì?(M?' ?y*ü,XμCrKSêèS*ü5ùètL?1?·G?J4§p?DBge2?10 ?7è NI-?è 24/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om ob_ a?)úe2?4§pJè ò6/è￠5à?·?E-?K?Nl?E-o1*+9,X_$ùà PowerBLAST (Zhang and Madden, 1997)è BLIXEM(Sonnhammer and Durban, 1994) ` BEAUTY(Worley et al., 1995)? E-?0′￡E?ZDB!¨EW,X?o ?VéèS*ü?G￡á à,X/?c19?kèAo,XμCM2 Y*ü?-è0J? ,hA1Z?·/?c10,X ??0èJèEY?,ìh,X ?D?!8êèaàhA1Z?· %0 ü,XêF?E ?Jè-1F'V)FS!??G??U,XèhA14§ ürP`?"?,X ￥)? `A?_(=,X ??o? 1×0′#] ?,X ?1 üf6(5%TS*ü?9?k?,XECê? ?5×[)? Altschul.S.I : (1991).Amino acid substitution matrices from an information theoretic perspective. J.Mol.Bio. 219. 555 565. Altschul.S.E. and Erickson.B.W. (1985). Significance of nucleotide sequence alignments: A method for random sequence permutation that preserves dinucleotide and codon usage. Mol.Biol.Evol. 2. 526 538. Altschul.S.E. and Erickson.B.W. (1986). Locally optimal subalignments using nonlinear similarity functions. Bull.Math.Biol. 48. 633 660. Altschul.S.E and Gish.W. (1996). Local alignment statistics. Methods Enzymol. 266. 460 480. 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Chandrasekharappa.S.C., Guru.S.C., Manickam.P., Olufemi.S.E., Collins.E.S., Emmert Buck.M.R., Debelenko.I..V., Zhuang.Z.., Lubensky.I.A., Liotta.L.A., CULSTAL.W ftp://ftp.ebi.ac.uk/pub/software/ DOTTER ftp://ftp.sanger.ac.uk/pub/dotter/ LALIGN.FASTA ftp://ftp.virginia.edu/pub/fasta/ BLAST ftp://ncbi.nlm.nih.gov/blast/ SEG ftp://ncbi.nlm.nih.gov/pub/seg/ NI-?è 25/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Crabtree.J.S., Wang.Y., Roe.B.A., Weisemann.J., Boguski.M.S., Agarwal.S.K., Kester.M.B., Kim.Y.S., Heppner.C., Dong.Q., Spiegel.A.M., Burns.A.L., and Marx.S.J. (1997). Positional cloning of the gene for multiple endocrine neoplasia Type 1.Science 276. 404 407. Clavrie.J.M., and Makalowski.W. (1993). Alu alert.Nature. 371. 752. Dayhoff.M.O., Schwartz.R.M., and Orcutt.B.C. (1978). A model of evolutionary change in proteins . 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Comparative analysis of the zeta crystallin/quione reductase gene in guinea pig and mouse. Mol.Biol.Evol. 11. 305 315. Henikoff.S., and Henikoff.J.G. (1991). Automated asscembly of protein blocks for database searching. Nucl.Acids.Res. 19. 6565 6572. Henikoff.S., and Henikoff.J.G. (1992). Amino acid substitution matrix from protein blocks. Proc.Natl.Acad.Sci. U.S.A. 89. 10915 10919. Higgins.D.G., Thompson.J.D., and Gibson.T.J. (1996). Using CLUSTAL for multiple sequence alignments. Methods Enzymol. 266. 383 402. Holm.L.., and Sander.C. (1997). Enzyme HIT. Trends Biochem.Sci. 22. 16 117. Huang.X., Hardison.R.C., and Miller.W. (1990). A space efficient algorithm for local similarities. Comput.Appli.Biosci. 6. 373 381. Jurka.J., Klonowski.P., Dagman.V., and Pelton.P. (1996). CENSOR: A program for identification and wlmination of repetitive elements from DNA sequences. Comput.Chem. 20. 119 122. Karlin.S., and Altschul.S.F., (1990). Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes. NI-?è 26/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Proc.Natl.Acad.Sci. U.S.A. 87. 2264 2268. Karlin.S., and Altschul.S.E. (1993). Applications and statistics for multiple high scoring segments in molecular sequences. Proc.Natl.Acad.Sci. U.S.A. 90. 5873 5877. Lipman.D.J., and Pearson.W.R. (1985). Rapid and sensitive protein similarity searches. Science . 227. 1435 1441. Needleman.S.B., and Wunsch.C (1970). A general method applicable to the search for similarities in the amino acid sequence of two proteins. J.Mol.Biol. 48. 443 453. Patthy.L., (1991). Modular exchange principles in proteins. Curr.Opin.Struct.Biol. 1., 351 361. Pearson.W.R. (1996). Effective protein sequence comparison. Methods Enzymol. 266. 227 258. 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Analysis of compositionally biased regions in sequence databases. Methods Enzymol. 266. 554 571. Worley.K.C.., Wiese.B.A.., and Smith.R.F. (1995). BEARTY: an enhanced BLAST based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res. 5. 173 184. Zhang.J.., and Madden.T.L.. (1997). PowerBLAST: A new network BLAST application for interactive or automated sequence analysis and annotation. Genome.Res. 7. 649 656. V T? NI ?? NI E¨ 2,? ) E¨ 29 X NI-?è 28/281×0′c?!¨í `DBge2? 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1×0′c?!¨í `DBge2? .htm Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om