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Email  íb±A?  ò4§p \G??U,X?¤x ??t ?EY??èW? à4§p?CK 2;4-*ü èJè*üb}10? ,S 4§p4?4?ê?Vp STS,X§8F'ú×?-1èFwhEg9?Y Chromosome Number,X  3?E- tμC?rt òECê#??t!7.BE2y,X6?o? )? üèú1/EYDB2l? _!7[?èJY¤xK? ò4§p?8 ü′úJsYE?E? Email 2 ;? Stanfordáu <1?2??,ìí ? ′?A?(=,X!5BE¨ 2 ò4§p?íb!￡t STSèáu <? y /?J?E￥,X ? ′?A?(=?J ü§8F' ` STS?A?(=,XC±/??1 centirays )!?? 1uJá¤o ò4§p,X ò6/è òμC? ?*üb `1T??Z*ü ,X STS,ìí Stanford òBT,XJW STS,X!5B,X ?##?? _ òB,ì4§ ü? CEPH YAC ò NI-?è 12/181 `0′T?2O `?2O@*ü(=)ú òBDBg,XS*ü 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 `0′T?2O `?2O@*ü(=)ú òB ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om 1993HèèT￠,X CEPH? Centre d’études du Polymorphisme Humain?èa Genethon ü0è ￥><Z?2O ? ′4?,X1??(=)ú òB? Cohen et al., 1993??E-? ò+′+G? ′ YAC4??è 6?E2yF?E￥ ? ′?A?(=,XEèX? YACG? ′ ?+′/?THHnèùà YAC?4? DEí"?? YAC fingerprinting??a inter Alu PCR4§px"??9; s!x? FISH? ` STS ?G￡ ò? 1u YACLZ ò?F?ú?>è??,X1 STS ?.,X òBó·èíb?Uùà CEPH YACgê1LZ  ?.,X ?h(=,X òNM,?E?Y*ü,X? +b YACg,XP? ü)[è ü?tE?E??4?"?ê inter Alu PCRx"?.Bn,ìfG? ′,X YAC Kèè!￡??!9 ?6?F?\ ?6?C?E? ? ′4?,X?t(=)úC±/?? ?bE-?&?è-áC±/?!¨KSC±/?è ? M4èE-?Vé?á9 CEPH,XA?5 “level”??t4{? level?EèXè+?tJ?n STS4? ?èWàh7??Y?t YAC,èyE2y?E-2OEèXèaGM6 STS ?G￡ ò*üb.Bn,ìF?G2?,XK ê )K,ì2O à? ?1A}-è0J5ù￠?t STSC? o?tè5à′L?C?E??) YAC/YACE2y&??,ì ?è?t4{EèXè+?tJ?n STS4??èá,èy+ )t YACE2yè5à+ inter Alu PCRê? 4?"?.Bn üù ?Wà,X?tê?t YACKèY?tG? ′è14{EèXL??UC?E??t YAC/YACE2y &??4{EèXL?C?E?t?4{L?C?E?tè11?1u!￡?/?,X ?M4??t4￡P`?A?aèE?E? í?+ CEPHDB,Xúd? J. Orlin, personal communication?k/4{êèP?ê ?6?á2? .B?5àLQ CEPHEèXE￥ 90á,X ?bKèC±4{,Xêè"? ￠ CEPHáu <k YACG? ′ CEPH ò ?1 üJ )!,X5% T ü4?9?k?E-G? ?R,XJòyY YACgμCè3Y?2?? òB,X aE@)[êè*üb ò,X QuickMapECêè1 ? ? s? òBDB,X[ê?##?? CEPH ò?Q,X0 *ü?"??EQ QuickMap[êè]>?Jy*üW9??,?DB[ê?' 5àè+b QuickMap ?í Sun workstations10èE-/??"??4￡á ?> ? CEPH3¤oJ\í QuickMap,X?/? ü4?+ M6è üE? E??Y Infoclone,XJòy? ?19?k?E-ê? ??NIè ?1¤x?t STS?ê?t ? ′?A? (=ê?t YAC,X á/??¤x á/? a? 2;YGbW,X s? òBDB?A1[CYJòyè ?1 ￠?t YAC,X )? inter Alu PCRxC?7? o?t? ?UkDBèú##?? <E2 CEPH,X5% T?E-? ? ECPH Genethon5%NI?)? üRJEY? I Jòyèy?9,X?NI??U4 ü?t?[?Eg9?t YACê?t STS,X á/?? YAChFI~1T? ,X plate_row_column?S +BfB???èV 923_f_6?íb STSè ?1*ü GDBúG!,X'é(? á?Vp ?k,XA±?êrP`xúG!,X-è0J á/??A1[ê ?J\í(Mn__è1Eg9 AFM20ZE3á?k!7.B,X á AFM220ZE3,X STS?3h"?? YAC  f,X á/?h?m? Y? Query?1A??KèVpA1 á/?, üb CEPHDBgèFw ü-á__ ?,ìμC,XNI M6ú??)??1?F?úùà?oGb STS,X'μCèVé(=c? ` ? ′ òBμC?1`F?ú 4-? STS,X YACe2?DB?A1F?ú?><,XY YACE?E?,èy PAC?￡  ￥)? ?YA1 STSè"?G? Alu PCR probe?vJ\?AèaE-t YAC ü inter Alu PCRxrP`>EY*üvJ\?1YF?úù ?a STS,ìF?,X YAC,XμCèWàa STS,ìLh?t inter Alu PCR,XC±/?? k?t YACT,X òμCè ? ü[?Eg9J á/?JY? QueryKè?)?,X+ M6ú?4-? YAC? FISH ` STS ?G￡ òDB,XìμCè1 ? inter Alu PCR `?4? DEírP`>!*ó?,XG? ′ μC? _ 2 G ? ′ _μC Genotyping information from Genethon 1994 genetic map: Number of alleles: 7 Heterozygosity: 0.78 NI-?è 13/181 `0′T?2O `?2O@*ü(=)ú òBDBg,XS*ü 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 `0′T?2O `?2O@*ü(=)ú òB ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Reference alleles: 1 4 Size range: 204è 206è 208è 210è 212è 214è 218 Primer sequences: ? ca? TGTACCTAAGCCCACCCTTTAGAGC ? gt? TGGCCTCCAGAAACCTCCAA YACs specific for STS AFM220ze3: YACs neighbouring STS AFM220ze3: !￡t YACA?5Y′t4ê-?a,ìG?_Vè ü,èy PCR?￡><èFAèa CEPHEˉ> rP`,X′ú!; 4§pè5à(Aèa )t?A?a,X YACè9$dbê ü?M2 CEPH?rP`x? ü YAC/YACG? ′><è a Aèa?t$é PCRG2?è5àIAèa?t?4? DEíG2??`H,X4ê-?><￠!bA1NIT,Xá à }Jòy5àk? CEPH YACg,X?t$L??+6Y? ±6ê+ "?"?L$nZ???Vp ?1kWè6?k YAC,X ??? ü¤o???è ?1R?úèE-"5§,X4§pèê ′ ü YAC|9  `LZ*óKSê DNA,XLc?L8E??,X?E-/????è? YAC,X??3?$è/?9? (Mn?2O§8F' òB L8 ? ′4?8× è òBêèA??t'§8F'(=)ú òB3+-è0JrP`x ` ? ′4??X?CK9Z? 678_g_3 CE ? 1730 kb? 746_e_4 CE ? 1080 kbè 1350kbè 1500kb? 756_e_8 IE Alu PCR probe ? 1380 kb? 765_d_4 IE ? 1060 kb? 765_e_4 IE Alu PCR probe ? 880 kb? 826_e_6 IE Alu PCR probe ? 820 kb? 56_c_5 a Alu PCR probe ? no size? 154_d_3 a Alu PCR probe ? no size? 261_c_12 a Alu PCR probe ? no size? 309_c_11 a Alu PCR probe ? no size? 340_g_10 a Alu PCR probe ? no size? 366_f_1 a Alu PCR probe ? no size? 682_a_11 f  ? no size? NI-?è 14/181 `0′T?2O `?2O@*ü(=)ú òBDBg,XS*ü 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 `0′T?2O `?2O@*ü(=)ú òB ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om ü\????èE-o òB6?!¨,ìh ? ′4?8× è òB¤oèAo,XμC?>< 12.1??ZE-o ò `￠5%T)? ?R><?L8E-G?/,X URLè ü GDB,X9$dNIM6T3 ?k?t??,X><? o ??><+ NHGRI,X5% ±,??M6A|A?,XY5% ?b>< 12.1? §8F' ò ? üt?k? The University of Texas Health Center at San Antonio¤ o ?b YAC,X?? STS ?G￡ òè1 ??t??x ò?E-t5% E?¤oGb òDB,X?t ò6??0 _+ M6è1 ??t ?b1A??,X ? The University of Texas3Y??§8F' ,X??x òè ü à?5%  ?1k? §8F',X STS ?G￡ òè ? 1280t STS ` 3300t YACè ?￠ Stanford Human Genome Center5%  Tk?E-? ò ??2?? aE@)[ê? s??￡DBE?á6? ü4?9?k? ü Sanger Centre3 ?k?+5%NIèEˉ5àkA14?4?,X??x ò `#cNM,?,XEˉ)?4 ?1k1&??? ò6, ü,X òBDB?#cDB3 ?1 Unix Tape 6??EQ? tar[ ê?? §8F',X òBDB,X?? ü5% !b NHGRI,X Eric GreenrP`x? ò?? STS/YAC ?G￡ òèLt1 EST? BAC ` cosmidG? ′5xDB?A1DBa Washington University,X§8F' 7 #cAu?,ìx6(? ü The Hospital for Sick Children,XF+?:2?3 ?1k?o§8F' 7 ,X(=)ú òB?NIE?E?G? ′§8F'(MY,X YAC4?>?5à??E-oG? ′ ?14D üS*ü inter Alu PCRx ò??4? DEí"? ` STS ?G￡ ò ò,X?"?9A?a??4z 90á,X 7q ?Ax+G? ′5x?Z ,a? Genome Therapeutics Corporation of Waltham, Massachusets?4￡ 0Z?Z,a§8F' 10,X ′tF?ú,X STS ?G￡ ò?E-o ò,X[),?U `?><￠A1@ ì,X5% T ?1kèE-o ò D ?6?E?E? GDBk? §8F' 11 òB ü University of Texas/Southwestern Medical Center’s Genome CenterFw G? ?1k?J??,X ò??J\í CEPH YACg ` roswell Park §8F' 11(MY YACg,X? t STS ?G￡ òèùà 1200t STS?4 ?1 flatfile6?ê GIF ò(6?kE-oDB? FISH  ò4§p ` YACa?t(Mn§8F'Bü2fg,Xx4§p3 ?1 üE-t5% TR? Yale University ` Albert Einstein Medical Center?4￡ ￥><Z??§8F' 12 ,X STS ?G￡ ò ? Marynen ` Kucherlapati, 1995??A1 ò?U1 CEPH YACg ?.è3ùàBü2f `JW2O _,XLZ? ü Columbia University ?k?2??§8F' 13,X òB?E-o ò+ CEPH YACLZ'4??èE-o LZ'E?E?S*ü inter Alu PCRxT ü?t§8F' 13(MnBü2fg>?#?9? üE-o1 LZ ?.,X òBT?35BZ ? ′? STS ` cDNA?E-o òB ` s?DB ü Columbia,X5% T ? 1kèWà1CYJòy>< ` flatfile,X6??E?? §8F' 16,X Los Alamos National Laboratory òB? Doggett et al., 1995???,ìí CEPH YACg `?2??Bü2f `JWLZ' ?,Xè ?Y 500?t STS,X STS ?G￡ ò? Los Alamos5% TY?t`<1A??,X§8F' 10 òDB,XDBgè1 ??2?? ò6+M6 ` ?Y òDB ,X flatfile? *ü,X Sigma òX?NM,?3+ Los AlamosEˉ> è1E-? ò3 ?1 SigmaDB[ ê6??E?? o?t ??rP`xè Lawrence LivermoreèBóB÷§8F' 19???P?úE|)[?1Bü2f ?.,X òB?4￡E?E?L$ ?úd ` FISH òX?CK9èW ?Y 400t STS `A?? ? ′?E-? ò ?￠ Livermore,X5% kè6??2??? _,X ò(? s? òDBQB5E?á6? ü InternetTk ? NI-?è 15/181 `0′T?2O `?2O@*ü(=)ú òBDBg,XS*ü 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 `0′T?2O `?2O@*ü(=)ú òB ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om ?2O§8F' 22+?t?BóB÷? The University of Pennsylvania,X ò(+ 200?tf ü CEPH YACgT,X STS4??? Bell et al., 1995?? üJ5% T ?1k`H,X?+DBè1 ? ?o 1A?,Xe2?NI? oêè the Sanger Centre in Cambridge, England3/?U ?tZE-5§8F' ,X òE?/?è????#c0??? ü Sanger,X5% T ?1k??1 CEPH YAC ?.,X STS ?G￡ ò?W6?ó1?2?? PostScript ò(6?ê5ù ACEDBDBgS*ü,X?/?????0′ a ,X?><?>?EQ? oêè Sanger!7 ü*üA??Bü2f? fosmids? BAC ` PACEˉ> §8F'LZ' ò( ,XX??!8DB)? üE?á6?￠ InternetTk? The Baylor College of Medicine1 CEPH YACg ?.è!7 ü 0??§8F';,X STS ?G￡ ò? STS1/EYDB ?￠?2??!b Baylor5% T,X5%NIe2?? oêè BaylorE?¤oZ?t + M6è*übe2?+?oJW9$d ￥><,X STS1/EYμC? ? aè ?b`H?2O4?2f'c?,X òB ?￠ the Department of Genetics and Molecular Medicineè Emory University?kèW MITOMAPDBg,X?F?ú? Kogelnik et al., 1996??E-t5%  ?1¤o?2??&??? ò6 ò(è/4?2f',X ? ′? ?? `G?4?è1 ? `H,X GLc?? T?2O òB9$d )? üíT?2O0(=)ú ò#|??,X &? Whitehead Institute/MIT Center for Genome Researchè5àè?? murine STS/YAC ?G￡ ò?4￡>X?CK9Z?E-? òè?4?ú ü 24000t YACT ?Y 10000t STS?)? ü?`? ˉúYè ü 1997HüêE?Z 7500t STS? MIT,X(=)ú òB ?1 ü Whitehead,XNIT ü4?##???Y? Mouse Genetic and Physical Mapping Project?T?2O ? ′ ò `(=)ú ò òNM,??,XJòyè' a ??$?|?YT?2O STS(= )ú òB,XF?ú?E-?F?úa Whitehead?2O(=)ú òBY,ì à,Xe2?NM `*ü + M6è?? x òDBE?á ?k? ü Whitehead5% TE? ?1k ?b 6331t1T ),ìF?KSz???,XT?2O(=)ú òBè1 ?E-? ò a Copeland/JenkinsL$ ?(?KSz??? ò,XH ü?E-o RFLP òè ü Dietrich et al. ? 1996?￡E?èúE|)[ 1.1cM?úE|)[èP?,XT?2O ? ′ ò!7+ European Collaborative Interspecific Mouse BackCrosNM,?k?A1 ò??,X)úA?úE|)[ú?E? 0.3cMèJè ?1 ü ECJMBC,XNIT ü4?k? 1997Hü?`?5§8F'? The Mouse Genome Database? MGD?+ Bar Harbor,X Jackson Laboratory4è?,X?t? _ T?2O ? ′μC,X@*üDBg?1uW ?TE??t ? ′ ògè MGDE?±+-Z\?(=)ú òB μCèùà4?62F+? òB ` synteny òèú9?okDB?tEˉ ?? MGD ? ü Jackson Laboratory,XNITk?Y??Y Mouse Genome Informatics,XJòyè' a?Y Mouse Genome Database,XJòyè ?k*übá à-è0J,X?tCK?5%NI? ü?EYNMùà,?)? 2?? ? ′ `?A?(=0ú ??2??1 ?????2?? ?ta òDB,XJòy\??óHCw??t?Y Maps ` Mapping Data,XJòyè ?1'?? NIèE?T?G￡,XT?2O ? ′ òBè3 ?1kE-?4?62F+? ò?1`tJòyè?Y Mammalian Homologyè¤oa MGD,X Synteny ò,X+ M6?E-o òè ?b?-1 ? ′,X!5Bè ?¤otê?t ?G2O*ó(=§8F',X"úE|)[!¨EW?'EY?E-tJòy aè?)?V ò 12.10/,X\KS,X? NI?A1NITY?t$?|?><? Primary Species ` Comparison Species? a5ù ü ò 12.10! b??G￠ê??!￡t?>< ?Y?2?? ?G2O*ó(=,X á/?? oêèY\?  3 ?o*ü 9L$n e2?5êèE?E?§8F'?4?62F+?:ú?ê?o′"á,ìG,XJWDBg  3?V05ù á?11? ?U*üE-t><è ?*ü1T ) EY??tY!¨EW,X*ó(=,X á/??L$ne2? ?Dè?Q ü>!¨EW*ó (=ê!¨EW*ó(=EY??t§8F'ê4?62F+?:ú?Y? Retrieve? a?? ü?ú?Js NI-?è 16/181 `0′T?2O `?2O@*ü(=)ú òBDBg,XS*ü 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 `0′T?2O `?2O@*ü(=)ú òB ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om aèú??)????/?*ó(=EY,X ? ′,X>< `Wà,ìí,X òB!5B? ü ò 12.11/,XEg? _è!¨EWe2?T?2O§8F' `?2O§8F'?1Y??kZ\?,X?t à$d  3? 1 120′¤,XYGA±Nl,X Internet9$d CEPH YAC ò http://www.cephb.fr/ceph genethon map.html CHLC ò http://www.chlc.org ECIMBCNI http://www.hgmp.mrc.ac.uk/MBx/MbxHomepage.html EntrezNI http://www.ncbi.nlm.nih.gov/Entrez/ Entrez<?? NI http://www.ncbi.nlm.nih.gov/Entrez/nentrez.overview.html GDBNI http://gdbwww.gdb.org/ GDB9$dNI http://gdbwww.gdb.org/gdb/hgp_resources.html Genethon FTP0-&? ftp://ftp.genethon.fr/pub/Gmap/Nature 1995 I.M.A.G.E. Consortium http://www.bio.llnl.gov/bbrp/image/iresources.html Jacksonr P`x http://www.jax.org/ NHGRI9$d NI http://www.nhgri.nih.gov/Data/ ScienceE@ )(= òB http://www.ncbi.nlm.nih.gov/Science96/ Stanford NI http://shgc.stanford.edu/ Stanford RH #A? http://shgc.stanford.edu/Mapping/rh/procedure/ Whitehead NI http://www.genome.wi.mit.edu/ Whitehead FTP0-&? ftp://www.genome.wi.mit.edu/pub/human_STS_releases C.elegans ACEDB http://probe.nalusda.gov:8300/other/ E.coli University of Wisonsin http://www.genetics.wisc.edu/ D.melanogaster FlyBase http://flybase.indiana.edu:82/ NI-?è 17/181 `0′T?2O `?2O@*ü(=)ú òBDBg,XS*ü 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 `0′T?2O `?2O@*ü(=)ú òB ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om V S.cerevisiae SGD,Stanford http://genome www.stanford.edu/Saccharomyces T? NI ?? NI E¨ 2,? ) E¨ 29 X NI-?è 18/181 `0′T?2O `?2O@*ü(=)ú òBDBg,XS*ü 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 `0′T?2O `?2O@*ü(=)ú òB ... 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