1 Y0′ ACEDB?t ? ′4?μC,XDBg ACEDB,X?8(M&? 6?C ACEDB(?/?4?<? C.elegansDBg ?/?>S"ˉh*ü,X1u)ú `¤o ? ′4?DB,X1K4??W  ü 1991H+ Richard Durbin ` Jean Thierry MiegOj¤o,Xèaà ￥)W9? `H)ú C.elegansNZ 3,X?8× èc? `(=)ú òB,X10? ü0′4§??,X ′(M5%C$d `Cm ??? `5? a4á,X/?c+ Durbin ` Thierry Mieg4ê `` XèE?YA??Ja? ?aZ·-? ,X4ê ?E-?êóè ACEDBE?*übA??|(= `á(=,X ? ′4?Au? [3]?ECêíbM2 ? ′4? ” μC,XAu??' YèAìVè!?￡á(=:,XDB? ? ′ Q á"??K',X[)1 [4è 5]??.B Au ?b ACEDBC$d,X?((=\ ?L?è 1997Hù,X±\Au [3] 25 30/?? ACEDB+bW,X?o(M?5à#> CK9?A1ECê!C ,XèJè ?E¤> ü Unix ` Macintosh OS2? 43?è Windows(P@T?|??DBg1?,X ò6+ M6¤oμCèùàYK'/,X ? ′ òBè(=)ú òBè?L·A?,XEèX `c?1?+ M6qM4,ìGμC,XCY4{[E2yèJ ?+ T????,X`??0?DB*ü#> ,XíB5,X6?Eˉ> 4?4?èS*ü??'s],X2O?Vè,ìG,X [)è ? ′è￡E?è `LZ,X DNA1?3A??G??U,X ,ACEDB6?\??,X+b?μC5à>G?? 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LM?T??oK MM?T??K RM?T?? ?K ?8,X ACEDB*ü E?E?##??CY[E2y,X[ê9xf,X?0 ACEDB?E-/?##????,X+ M6V ò 13.1?E-￠ Grain Gene??t*üb Triticeae,XDBg? a9,Xù ?Y?t ACEDB0k ·,X L ü0k ·?E-o0k ·?2??T???0{*ó,XèV*ü n!?/?á à,XμCY?? ACEDB,X 0k ·!b òoT,X0k ·èW üECê??ê/?9?J?YDB2O _*üb1Kù?&? NI-?è 1/121 Y0′ ACEDB?t ? ′4?μC,XDBg 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 Y0′ ACEDB?t ? ′4?μC,XD ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om ? Reference2O _? ?? LM?'??t KeySet0k ·èJ??ZA?? Reference2O _,X? ,è E-o? ,>/?íB5?&???t referenceíB5èV BCG 28 487èí'?Z1Yt0k ·? ?? 0k ·?/[),X4?8VèV05ùèNl,?1?&???)2k'+F? ?'?,ìh,X0k ·? ò 13.1 ò 13.1E?ùà?tF+? òB?o???E- ü reference0k ·?è&?? locus?,X iBgl ? ,X?E-??,X*ü ò66?￡E?DB,X??,X?/?? ò,X locus?A?3CY[E2y,Xè ?E?E?&??? LM?^W?#?[0k ·F+? òB?,XDB ???,XèB5 ü reference0k · ? ? üE-/????èVpYE?',XDB ` ò6èDBg1u)ú ,^ locus?A?A?5B?5,? èSW/F+? òB? Gb ACEDBE?Y?o ü4?,X-6??E-G?(M?|9$ ü Sydney,X%??ynF+?μC?,X Bruno Gaeta¤o,X?t [7]? Cornell?:,X Dave.Matthecos!7??¤o?tGbF+? òB/,X- 6?Y? [8]? a?úèAo4?,X?4?c?/,X(M&?? DB? _ ACEDB,X ?F?úDB? _?ê ò6?èE-ú?nDBg,XDBV)4?4?,X? ?t ? _ ?+1T4?,XAá??t1?·G?èE-+DBg1u)ú ,9n? ?2ODB ?+V sequenceè geneè reference12O _9></? ?? _1V ò 13.2,X6?±, ü á models.wrm,X[[ê èW, üDBg,X wspec,?)?? ü[èA?n/DB,X? _Y\,X&I#?è ü JW,X ACEDB,X/ [9]èL??U ?? _ùàYNXnQ,X4§X?E-?Y?ú ü a[,X c?/t1A|A??"??èèAo4?,X? _?4? ` ACEDB? _,XK?Nl ??? [10]?A?5ù ? 1Kùá à,XDBg9Z?·? _,XE?*ü8× è? //this shows partial models for the Sequence, //Locus and Paper class ?Sequence DNA UNIQUE ?DNA UNIQUE Int Structure Length UNIQUE Int Properties Pseudogene CDS Genomic_canonical Locus ?Locus XREF Sequence Paper ?Paper Remark Text ?Locus Sequence ?Sequence XREF Locus Paper ?Paper ?paper Title Text NI-?è 2/121 Y0′ ACEDB?t ? ′4?μC,XDBg 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 Y0′ ACEDB?t ? ′4?μC,XD ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om ò 13.2 modles.wrm Z)/?o? _,X(M&?è?à,? sequenceè locusè ` paper informationE-Y/?μC ,X? _?E-o_$Já6?)/E-/?? _Aá??,X<F?s6?è6?)/?oG??U,X(M&?? ? _Y?t14{?,X4§XèB5V?U ò? èDB??bàè' a"?A??C?Eˉ> ?)úè C^úC^4??￠?,X ??è!￡?t&?F?·><?2O?L ?DB,X?t$ú>??!￡?C?? ?b?t?/0úèê!84?4§êúYA??+!?èE-G? ??9DBè Structureè CDSè ` Paper ?/0ú,X_$è Intè ` ?Paper+!?? ￠oT?? ???)ú?E?E? Sequence? _,X_$? ò 13.2??à ?1,??èE-/?? _ ?A} DNAc?,XY? à?t DNAíB5èW,XKSzè ?c??Bü,X2O _?E-G?,X Properitiesúú Y?6(2?CK9?E? ?-1F'c?,XFw?F?ú?> ￥×Zè úY?t!&?aWíhè ú?U íWEˉ> ?T?(M?,XAèa? E-?2O,X1?t+!?? ?Sequence?>,?tíB5 á,X,| <?íB5 á?U6?> ?A??èA? ?ac?,ìG,XJaμC?UE?E?íB5 áLt4-íB5,X?Ja2O _,X+!?Y? Text?y ?7?+ ?,X[?è Float?*üb#B&? _DB?è Date?*üb1ó?è Int?*übH _DB??? /?+!?2O _L$ ?9J,XDB2O _?_Vè?tKSz _? length?6??9 100èá6?? one hundred?05,?è?t+!? ?á D9y ??G?DB,XEg9?E-E? Y*ü,Xè? tc??#] ??G?,XA?[? Paper?è!&?? loci?èA?A?? remarks??è UNIQUEL$  ?t+!?í!￡?tíB5 ?6?Y?/?Eg92O _? ü_è?t sequenceíB5 ?6?Y?tKS zè+!?E?Y?,Xèá,XL$ èE-G?á ü#] ?? E?E?S*ü+!?èWày ?íB5 áè^μC ü ACEDBYfE2CK9??!4?A??E-o+!??5× <% sequence? _,XúèWà^?tc? `?t?((=6(2?CK9? Paper ?paper '?t(Mn,Xc?^DBEg9 ?Paper+!? aè SequenceíB5 `? Q á,X PaperíB5?? 0?CKJòyèA1JòyE?E? ü*ü + M6&?? Link9?0?V ò 13.1/?èE-/?Jòy ) ?,X?<Q' ##??5ù ü SequenceíB56??? ?&??,X2k',X Paper+!?è,ìh,X PaperíB5 H á6? 26( Sequence?áE?èA??DBg1u)ú ,F? ?*ü ¤o ? ?,XJòy? ?E?E?S*ü XREF? cross reference?97?|?0???t Sequence? _,X XREF,X_$? Locus ?Locus XREF Sequence ?t XREF+!?Y?tF?úè XREF!M6,XF?ú,??2O? class?èE-G? ?LocusèE-G?W ?NO class XREFs5àá6? Intè Textè Floatèê DateType2O _? XREF aM6,X,???A? 0ú? target tag?èE-t?A?0úAèa ü,??2O ?o+!? ?*üb?0?xf?,XJòy?Z E¤> XREFè Locus2O?NO?E-/?JòyèE-? G- ü ?Locus+!?è?tC??NOùà Sequence,???A?0ú `Y.Bn seqenceíB5,X+!??E-C?? Sequence ?Sequence $μC?E-t?U"è' SequenceíB5,X ?Locus+!???9DBè XREF?0?xf?,XJòyè íbDBg,X1u)ú ,èE-ú??1Tê ? ?Jòy,X1u)ú?èEˉ?!9,X?0?< ? ?JòyèSEg 9?)+!?,XDBF?6??0? ? ?Jòyè?U0E-?&?è?àhV?? Locus? _? Sequence ?Sequence XREF Locus ?*ü??6? //í? _Eˉ> "??·è?' models.wrm[ê>A?9êè?>  // ?{,XY?ú> ?+9??"??· ü4êm models.wrm[ê `DB[êêM2 Y*ü? NI-?è 3/121 Y0′ ACEDB?t ? ′4?μC,XDBg 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 Y0′ ACEDB?t ? ′4?μC,XD ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om DBEg9 DB1? _.?SEg9 ACEDB,X?DB[ê ?E?E? X windows+ M6ê,èyE?E?DBg ,X Q?> ^DBEg94-DBg,X [12]?E-t Q?> + M6èE-G?<Q' á?4?è ü￠Ja? )úE?/?,X7?| L?DB?0 H\G??U?E? ? üE¤> DBgêè,èy?0?t?,XíB5?EY*ü ?T??"? a?bDBg1u)ú , ?D,XEY?èDBG￡,X?? `DB,X?Bü??U^?G￡DB￠[ êP?,XEg9è\??S*üxf?,X?"??'[ê>A×*üêè,èySDB?êèE?Y ?t!?:9￡E?E-tíB5?*ü0N> 9ú?!￡?t!?:?è!￡!?,X1?> 2O áèy? 9íB5 áè1+!?E?E?bWà,X?A?0ú0?>A???t9 sequence ` paperíB5è? àhV ò 13.3Eg9DB? "??è?àá*ü?m!￡?t+!?è?A?0ú3á*ü à? _/,XNNc? ?è?A?0ú+ !?,X4? ü?NO0ú ü? _èVpá0ú üèDBg?Ax?Jí?1è Genomic_Canonical? _ aá6?C3Y+!??Fwo ü à?> ?A?0ú aC3YJaμC,XEg9DB3JíA?,X?è4?7è ,XGbDB>?EQE?/?,XA|A??? [13]èWùàY?? _DB[ê,X??èDB,X?L8è ?íB5 è á1Y?? 1A? `>< ACEDB*ü1A?,XAá??9h*üíB5,X?Bü `á àDBKè,XJòy?Y′t1A?,X+ M6?4?[,X6 ??E-G?á#] ??è?t s?,X1A?1Kè*ü ü1A?ê,èyEg9 Q?× “query be exmple?8×_?1A??1KèWr)?s6?B5?><? × “query builder?1A??? <?èW}*ü .B?,X?0?á,X1A??1A?,X ?h?2??0ú üK'??,XíB5 á ?TTV ò 13.1,X KeySet?è1A?,XAá"? ?n,Xè ü"?,X1A??NO `? _,X4§XP ?è ′!8 Find Sequence ATHFOO1; Follow Locus ?t ü"?,X1A??V!E?èWíhb? _ `DBèE-t1A?,X 2hú??6(2?E-?c? ,X!&?è1A?è Find Sequence AGE>10 <Q' 0ú üAá"?èJíA?,Xè ′ Sequence2O"uY Age?A?0úê,ìG,X+!??Vpè?n?U E- 1A?è ?+DBg1u)ú ,? ? _è#?t,ìh,X+!?? ACEDBE?ùàY TableMakerè?t*üb??,ìG><,X1K?W,X+ M6\áès6?èW ?A}*ü ￠Y6(,X ?2Oé*ü `G6(μC?,ìíb!E?,X1A?è TableMaker6?/ ` ?2?íB5,XμCè5àá ???íB5 á?Gb1A? ` TableMaker,X-:Y??? [14 16]? Sequence"ATHFOOI" Length 3879 Remark " a very strange Arabidopsis thaliana DNA." Locus "FOOI" Genomic_canonical Remark " a second remark" Locus "FOOI" NI-?è 4/121 Y0′ ACEDB?t ? ′4?μC,XDBg 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 Y0′ ACEDB?t ? ′4?μC,XD ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Paper "smith_1997_aahmt" ò 13.3 ACEDB,XDB[ê S*ü? `]>? ACEDB ?￠A??!C A?K?,X FTP0-&?9?k? ü?? ￥×úY Unix2?43,X]>?Aèa?E? ? FwoL??UG??4êEe,X*ü ¤oC$d·-??Gb FTPaG ?,XAo4?Y? ?￠!M6?¤,X FAQ R [3]?0′,XY?Gb( 4.3,X ACEDBè3m0:ê,X#> (??,XY??nó ,X?( ü ACEDB,X?K?4? [17]? ACEDB,Xc?úd éA? ò6?,X/ ?A}*ü ü ACEDB?Eó L? `údF+?DB?<Q' Map ` Clone Gird/è Wà?F+? òB `(=)ú òBNM,?èJè ?*ü ò6/9·><*ó(=ê:,XEèXè?à,XG? &? üc?úd? c?,X/?/?(MU òB? Feature Map,Fmap?èWJòyYA??1KèJùà Gene Finder[18]× Blixemè?/? BLAST[19]?G?!¨í??3 < [20]× DOTTERè?/?&? ò/?c [21]? 4D üE-o1Kèc?úd¤oZ?,X ò6)?W? <Q' A?Au,X?8?A?97?b?U? _2?43è+? s'è `?2O,X,Xc?*ü2O,X?"?Eˉ> ú dè Fmap???4?<?? C.elegans? ? ′4?c?Au?5à? ￥,X? (MU òB (MU òB?/?P?z ?G!5B,X/?è ò 13.4/Z??97?b Schizosaccharomyces pombe,X rhp6,X?G!5B,X/ ??A1 ò))?ZE-/?/6?,X?84§X?NJF?YJ? `[ +è?M681F?èE-o?￠o ?*ü4v8FD+A?Dè?M6￠o ?t1?·G?? 1. Locator: 4ó8F,X/`Hc?,X<?,QBü?T￥8F5??/?|?0E?E?? a)Zoom Inè Zoom Outè ` WholeYJ?× b)&??4ó8FJ$￥|? LM?× c)&??n!5?,X ?{ ? MM?è&??n!5,Xo{? LM? ?$?| `4y? 2. Sequence and ends?<,Q8F `c?íB5 á? EM? 250728?è<,Q></′t sequence?c??íB5G? ′5x,Xx ′F?ú?_"uYx ′?? 3. Summary?T?JaNp8F5Aפo(M??D8F5+ nZ#? ? 4. Scale?*üNJF?,X Origin[Eg9èG?nA?D￠c?,X &???? 5. Genes? “Genes”E-G?></`H,X `F?ú,XE@)c?>V mRNAê4ê-?c?? CDS?￠E@A￥ CK??-?$4?!6?-?$?èW?n-ê$4§X@?ê$?D8FEB§? `Y#?$ ?4?56(-ê$?>/?? 6. Features: k4v8FaL8Z6?￠ EMBL(MU>< ` GeneBankA?)R,X ? ′1ê,X(MU,ì 6(2?? 7. ATG??T?8F·><-!￡tKùA?,X%0 ü,X+.?!üGLE@A￥CK??-?$èE?E?G!5B GeneFinder><è ??E?Ja,X?-?$? NI-?è 5/121 Y0′ ACEDB?t ? ′4?μC,XDBg 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 Y0′ ACEDB?t ? ′4?μC,XD ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om 8. ORFs?T￥8F"G4?·><!￡t?KùA?,X4?!6?-?$?1`KùA?E-G?3>/? 9. Gene Finder Coding Sequence?&D8F></P?V)[<?,Q4ê-?,X  3? 10. Coding Frame?D8FEB§></KùA?è￠ ?k!￡tê$? 11. Pssearch?M&8F></,ìí PROSITEDBg?t ?,X G!? 12. BLASTX?D8F·><F????í8V!?? maximal segment pairs,MSPs?? ü BLAST /? HSPsè??1×0′??W97?<?,QBüc?,X BLASTX1R? 13. Gene Translation?E?E?&??? RM? ? ′ ?|?E@) `§8FèE-ú?#?t9° )èEY a EYNM Show Translation ` Color Exons? 14. BLASTN?T?8F·><￠ 8?DBg,X BLASTN1R1?,X MSPs? 15. INTRON_HMM?# ,T?8F·><Z?tY#?$NX#/?c,XNX#4§p? 16. Gene Finder Splice Site? ?T?,X&?4?·><>y ?'è??&?4?>yo'??§ 8F,X!&? ü à? !?Y?è ü ? ′NX#êú*ü4ó8F,X(MU?>y!&?è ATG?-?$è ` !6?-?$?? 17. DNA Sequence? 8?c? `J à1(=?.? ?? Base?>A?5BE@A￥CK??-?$$?+.? !üGLè ATG??E?E?EY?&?? ? ′? RM?{*ó,X9° ),X Color ExonEYNMèê$P???T? 8Fè>y,X?-?$?2?8Fè4?!6?-?$?4v8F?c?￠ 5’ 3’0?è&?? Rev CompYJ? ? LM? ?/,ìh,X??? Rev Comp9° )? RM?YEYNM9 )(à ?E@ `>9c?? ò 13.4 Sequence?c??íB5,X/?UE?E?&?? 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FmapEg9c?5àá*ü,èy m9DBg?è?Uúdc? ?TE?L??Um9DBgèèEg9,Xc?0? t ace[êèV?_/? Sequence "MysortSeq" DNA "MyShortSeq" Dna "MyShortSeq" cacacacaccacacccacaccacaccacaccacacccacacccacacacaccacacccac acaccacacccacaccaactctctctcatctacctttactcgcgctgtcacaccttaccc ggctttctgaccgaaattaaaaaaaatgaaaatgaaatcctgttctttagccctacaaca E-t[ê???t Sequence(c? íB5 `?t DNAíB5?EY? Sequence(c? íB5 MyShirSeq ? ü Fmap?/ Sequence(c? íB5 ` ?/ DNAíB5?<Q' èE-?/?M2 áé?"??,X ?0èM2 G??U,X?U"??DBg?-1F'?U???t Sequence(c? íB5èJè GL c??L? ` Sequence?c??íB5?F'/?Vpc?íB5 MyShirSeq1[?/èí ?V?? MyShortSeq DNA MyShortSeq 180 NI-?è 8/121 Y0′ ACEDB?t ? ′4?μC,XDBg 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 Y0′ ACEDB?t ? ′4?μC,XD ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om c?,XKSz>Au1k?èJ>7?||9íB5C3 ü DNA?A?0ú a,XH _+!?? Int field? G??E-G?,X DNA? “Magic Tag”?E-o(M!^,XA???A?>m9DBgé￠è?U*ü9 /,?,X??/? Magic Tag ?DBg,X? ￥5ù `c?úd5ùL??U-1F' ?o?A?>*ü9{*ó ACEDB/2?,X? Magic Taga >S*ü,XAèa?? 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MSPcrunch ￠,ì à,XDBgc??#yE￥,X MSPsèE-?#±+-"ú,X MSPsèWàa ?tú5 ·,X?,ì?7è? 4. MSPcrunchúd BLAST,XEg?èJ{*ó ace[ê? E¤> 1 3S BLASTM2 &I#èèVp?U"?tDBg,XY MSPs1A?è MSPcrunch ?>*ü 0úYZ?G,X ace[ê*ó? <? ü@E  3,X<c?,X BLASTYF?1A?èL??UíAu1kC$dY?G￡,Xi9è ′c?DBg,X?? ?×L??UKS,XêKè91R×E?L??U4è?1?r?,XDBg?áE?è?G￡,Xc? ?E?E?5%NI¤ o,X BLASTáu9kúd? BLAST,XEg? ?> E MAILé*üèè MSPcrunch ?*ü0{*ó ace[ ê? NI-?è 9/121 Y0′ ACEDB?t ? ′4?μC,XDBg 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 Y0′ ACEDB?t ? ′4?μC,XD ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om ò 13.7 GeneBank  ace,XAá"?úd/?c,XEg? t94§p? üc?,XúdèM2 ??U,X￠??,X1R `NX#/?c.BAx4§p??) ?.BAx 8?c?  3,X4§pF? ?>Eg9 ACEDBèJ ?/ Fmap[êT,X?tY§8F,X?? ' Fmap,Xc?íB5>/êè ?t )(à,X#] ? Sequence(c? íB5,X “Method(?"? )”4?  ?t4§p?? Methods(?"? ?n?4§p?a >/ `a > Blixem?)ú,XíB5?E-G? 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Sequence MyShortSeq Feature INTRON_HMM 100 120 10.12 ?/? #? ü úds6? NI-?è 10/121 Y0′ ACEDB?t ? ′4?μC,XDBg 2005 1 18file://E:\wcb\*ó(=μC:?A￥??1 Y0′ ACEDB?t ? ′4?μC,XD ... Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om Click to buy NOW! PDF XCHANGE ww w.d ocu track.c om &?EY AnalysisYJ? ??# (LM) DNA Analysis (DNAúd 0k ·èA10k ·¤o??,XY*ü,Xs 6?èE-os6? ?h*üb DNA0k · `íB5,X KeySet?0k ·?èE-G??4??o *ü,Xs6?? 1R ? `?E?±6ê ? ?E?E? ü DNAúd0k ·,X[Eˉ91RKó? `EY? DNA?1R 8?c??ê AA? 1RE@A￥ 8?c?? üc?n!?Y ENTERK??1R? ü KeySetT1RèE@?,Xù ?Y ?,Xc?,X KeySetè*üb DNA0k ·,X1R^ FmapT,X ? ?=8F? ü?/???è1R ,X4§pF?tEQ DNAúd0k ·? ?1RKó? ?ùà 8?.? ?,X??-?$? ü4?}[ê “DNA and amino acid nomenclature”?Y DNA `!ü ?GL,X??-?$? ? ?>,|? MotifíB5,X2O _è5à aè Y ám9  ?E-?t ace[ê,X_$? Motif "Branch" Match_sequence yTrAy Remark "Splice branch site consensus" Remark "Y is pYrimidine, R is puRine" DNA Motif "HaeI" March_sequence "wGGCCw" Offset 3 Cleavage "wGG'CCw" Overhang 0 Remark "unpublished observations" DNA "?? 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