BIOTECHNOLOGY BY OPEN LEARNING Biotechnological Innovations in Chemical Synthesis PUBLISHED ON BEHALF OF: Open universiteit and University of Greenwich (formerly Thames Polytechnic) Valkenburge rw eg 167 Avery Hill Road 6401 DL Heerlen Eltham, London SE9 2HB Nederland United Kingdom �&-=-= EINEMANN Butterworth-Heinemann Linacre House, Jordan -1, Oxford OX2 8DP A division of Reed Educational and Professional Publishing Ltd. -@ member of the Reed Elsevier PIC group OXFORD BOSTON JOHANNESBURG MELBOURNE NEW DELM SINGAPORE First published 1997 8 Reed Educational and Professional Publishing Ltd 1997 All rights reserved. No part of this publication may be reproduced in any material form (including photocopying or storing in any medium by electronic means and whether or not transiently or incidentally to some other use of this publication) without the written permission of the copyright holder except in accordance with the provisions of the Copyright, Designs and Patents Act 1988 or under the terms of a licence issued by the Copyright Licensing Agency Ltd, 90 Tottenham Court Road, London, England W1P 9HE. Applications for the copyright holder’s written permission to reproduce any part of this publication should be addressed to the publishers. British Library Cataloguing in Publication Data A catalogue record for this book is available from the British Library Library of Congress Cataloguing in Publication Data A catalogue record for this book is available from the Library of Congress. ISBN 0 7506 0561 8 Composition by University of Greenwich (formerly Thames Polytechnic) Printed and Bound in Great Britain by Martins the Printer, Berwick-upon-Tweed V The Biotol Project The BIOTOL team OPEN UNNERSITEIT, THE NETHERLANDS Prof M. C. E. van Dam-Mieras Prof W. H. de Jeu Prof J. de Vries UNIVERSITYOF GREENWICH (FORMERLY THAMES POLYTECHNIC), UK Prof B. R. Currell Dr J. W. James Dr C. K. Leach Mr R. A. Patmore This series of books has been developed through a collaboration between the Open universiteit of the Netherlands and University of Greenwich (formerly Thames Polytechnic) to provide a whole library of advanced level flexible learning materials including books, computer and video programmes. The series will be of particular value to those working in the chemical, pharmaceutical, health care, food and drinks, agriculture, and environmental, manufacturing and service industries. These industries will be inmasingly faced with training problems as the use of biologically based techniques replaces or enhances chemical ones or indeed allows the development of products previously impossible. The BIOTOL books may be studied privately, but specifically they provide a cost- effective mapr resource for in-house company training and are the basis for a wider range of courses (open, distance or traditional) from universities which, with practical and tutorial support, lead to recognised qualifications. There is a developing network of institutions throughout Europe to offer tutorial and practical support and courses based on BIOTOL both for those newly entering the field of biotechnology and for graduates looking for more advanced training. BIOTOL is for any one wishing to know about and use the principles and techniques of modem biotechnology whether they are technicians needing further education, new graduates wishing to extend their knowledge, mature staff faced with changing work or a new career, managers unfamiliar with the new technology or those retuming to work after a career break. Our learning texts, written in an informal and friendly style, embody the best characteristics of both open and distance learning to provide a flexible resource for individuals, training organisations, polytechnics and universities, and professional bodies. The content of each book has been carefully worked out between teachers and industry to lead students through a programme of work so that they may achieve clearly stated learning objectives. There are activities and exercises throughout the books, and self assessment questions that allow students to check their own progress and receive any necessary remedial help. The books, within the series, are modular allowing students to select their own entry point depending on their knowledge and previous experience. These texts therefore remove the necessity for students to attend institution based lectures at specific times and places, bringing a new freedom to study their chosen subject at the time they need and a pace and place to suit than This same fi.eedomis highly beneficial to industry since staff can receive trahing without spendmg signhcant periods away from the workplace attending lectures and courses, and without altering work patterns. vi Contributors AUTHORS Mr J A M van Balken DSM Research, Geleen, The Netherlands Dr T G Cartledge, Nottingham Trent University, Nottingham, UK Dr J Green, Napier University, Edinburgh, UK Dr J F Hall, De Montfort University, Leicester, UK Dr R 0 Jenkins, De Montfort University, Leicester, UK Dr Ing J Kamphuis, DSM Research, Geleen, The Netherlands Dr C K Leach, De Montfort University, Leicester, UK Dr G Mijnbeek, Bird Engineering bv, Schiedam, The Netherlands Dr J Verweij, Gist Brocades bv, Delft, The Netherlands Dr E de Vroom, Gist Brocades bv, Delft, The Netherlands EDITOR Dr R 0 Jenkins, De Montfort University, Leicester, UK ADVISORY EDrrOR Dr G Mijnbeek, Bird Engineering bv, Schiedam, The Netherlands SCIENTIFIC AND COURSE ADVISORS Professor M E C van Dam-Mieras, Open Universiteit, Heerlen, The Netherlands Dr C. K. Leach, De Montfort University, Leicester, UK ACKNOWLEDGEMENTS Grateful thanks are, extended to authors, editors, advisors and production team. Especial thanks to Ms K Brown. The development of this BIOTOL text has been funded by COMER, The European Community Action Programme for Education and Training for Technology. Additional support was received from the Open Universiteit of The Netherlands and by the University of Greenwich (formerly Thames Polytechnic). viii How to use an open learning text An open learning text presents to you a very carefully thought out programme of study to achieve stated learning objectives, just as a lecturer does. Rather than just listening to a lecture once, and trying to make notes at the same time, you can with a BIOTOL text study it at your own pace, go back over bits you are unsure about and study wherever you choose. Of great importance are the self assessment questions (SA@) which challenge your understanding and pmgress and the responses which provide some help if you have had difficulty. These SAQs are carefully thought out to check that you are indeed achieving the set objectives and therefore area very important part of your study. Every so often in the text you will find the symbol II , our open door to learning, which indicates an activity for you to do. You will pmbably find that this participation is a great help to learning so it is important not to skip it. Whilst you can, as an open learner, study where and when you want, do try to find a place where you can work without disturbance. Most students aim to study a certain number of hours each day or each weekend. If you decide to study for several hours at once, take short breaks of five to ten minutes regularly as it helps to maintain a higher level of overall concentration. Before you begn a detailed reading of the text, familiarise yourself with the general layout of the material. Have a look at the contents of the various chapters and flip through the pages to get a general impression of the way the subject is dealt with. Forget the old taboo of not writing in books. There is room for your comments, notes and answers; use it and make the book your own personal study record for future revision and reference. At intervals you will find a summary and list of objectives. The summary will emphasise the important points covered by the material that you have read and the objectives will give you a check list of the things you should then be able to achieve. There are notes in the left hand margin, to help orientate you and emphasise new and important messages. BIOTOL will be used by universities, polytechnics and colleges as well as industrial training organisations and professional bodies. The texts will form a basis for flexible courses of all types leading to certificates, diplomas and degrees often through credit accumulation and transfer arrangements. In future there will be additional resources available including videos and computer based training programmes. BOOKS IN THE BIOTOL SERIES The Molecular Fabric of Cells Infrastructure and Activities of Cells Techniques used in Bioproduct Analysis Analysis of Amino Acids, proteins and Nucleic Acids Analysis of Carbohydrates and Lipids Principles of Cell Energetics Energy Sources for Cells Biosynthesis and the Integration of Cell Metabolism Genome Management in Prokaryotes Genome Management in Eukaryotes Crop Physiology Crop Productivity Functional Physiology Cellular Interactions and Immunobiology Defence Mechanisms Bioprocess Technology: Modelling and Transport Phenomena Operational Modes of Bioreactors In vitro Cultivation of Micmrganisms In vitro Cultivation of Plant Cells In vitro Cultivation of Animal Cells Bioreactor Design and Product Yield Product Recovery in Bioprocess Technology Techniques for Engineering Genes Strategies for Engineering Organisms Principles of Enzymology for Technological Applications Technological Applications of Biocatalysts Technological Applications of Immunochemicals Biotechnological Innovations in Health Care Biotechnological Innovations in Crop Improvement Biotechnological Innovaeons in Animal Productivity Biotechnological Innovations in Energy and Environmental Management Biotechnological Innovations in Chemical Synthesis Biotechnological Innovations in Food Processing Biotechnology Source Book Safety, Good Practice and Regulatory Affairs