? 21c y¥%=??/
?
B a'àQ
?(B )y?? (y? )|?÷?y (yF
DNA) ??%?¥/
??1y? (gent
transfer)?yM9e?1?? b
^ù? y
Vr a2???¥×1ù?
m
b
?(= )y?¥? ?
?1 a??8?
1?¨%μ?
2%A±
?
E|??8???%= b
?2 ay?
1 y /
?
3 2 %
2
y ?
8%
?áE a?é8o?E aè
E (è, d/
? ) aDEAE?
E a%A±?
E
hy8o?E? b
= at ? a?DZE¥y/
?
1 a¨
?á —DNA??E
?2 a?é8o?E
3 aèE (è, d/
? )
?4 aDEAE?
E
5 a%A±?
E
??
?[
?á -DNA??t¥?
TC
H V
P DNA
?%V
? ??%}?
|YV%?M
l
ê
H
7¥ b#é?%=é?%¥ DNA?μ
1% j5% V[é?%????μ?? 1%¥ DNA V
[D% DNA??%?é?×?Vry?
¥?
qv?1 10
-4
?[/
??¨??? DNA???
??té?
H?Vréù?¥ù? bNZE?
LC%?
^Kè¨i
nê¥ZE b
)
??é8 (lipofectin regeant,LR)
k4
^ ?0?é8 N-
[1-2 3-Dioleyoxy, Propyl]-n, n,n-
Trimethylammonium Chloride(DOTMA)? Dioleoyl
photidye-thanolamine(DOPE)¥?t [1:1(w/w)] b
?
a¨?ü DNA????LC?!%?
^"-
Hq/KZL¥?ZE-B b?
qúa?
?á
E1
?ú 5 j100?ü DNA? RNA??ò?%
b
)
?DEAE-?
o?¥?e ???b# V?
^YV=}
`T¨7
P DNA?é?%?NE
o
a?
H?
L?r
qD DEAE-?
i
[#%D DNA/DEAE-?
?A¤(
HW¥
é?μ1" V?¨?úi¥ DEAE-?
(1g/L)T¨?
HW (30sò -1.5l
H )? V¨?
?i (250g/L)¥ DEAE-?
T¨?é
HW (8l
H )
)
?'E3¨+
y
!? (è, dN )ü??
?¥
s8%?8 DNA]b?
?? (? )
@[úaè
?
?\é DNAYV%
7é?=i V???%¥ DNA?
P%
?
3? b?M¥ DNA1{μê4S:
y¥é ì9 Vê4?!?é?k?
)
?'E
^1ùA±?
°¤ü DNA?
??=
P-
?
3???
s8%yF?¥/
? b'/
?31μ
?
áí )¥ê?
i[
?í 7?Tê?]
H1?μ
1? ??N[?!A±?B?¨
m? ?? ??
H
1?
P?
μB? 0.6cmé¥%?
té^|
?U 7 g1 2 j30 mW? gl? 10 m÷D?U
7 gv? 50 m^O
% b
? aI
chy8o? DNA?
?I
chy8
? RNAhy5?"t*ù
y3
%?|"¥yD8×F
8é?
cDˉ?á
3???Lhy?a
??
ó?%aVr"¥?a b
+?
1??
qú
2 V??Vro??s
%
3"¥yvl
sK
él? 8Kb
1 a?%k?
?1 aS:y¥_?? neoy? LacZy
?2 a′y_??1á
3??8ss
M9íK92
?3 a"¥y_?¨ Sourthern Blot+?
?4 a| Alu?
s
?5 aSV
40
v T Fey_?
?6 aèj??!á
??_?