Massachusetts Institute of Technology
Harvard Medical School
Brigham and Women’s Hospital
VA Boston Healthcare System
2.79J/3.96J/BE.441/HST522J
INTEGRINS
I.V. Yannas, Ph.D. and M. Spector, Ph.D.
UNIT CELL PROCESSES
Cell + Matrix Product + Regulator
Regulator
Mitosis
Synthesis
Migration
Contraction
Endocytosis
Exocytosis
UCP
Connect.
Tiss.
Epithelia
Muscle
Nerve
ECM
Adhesion
Protein
Collagen
Biomaterial
Integrin
The Cell and Its Membrane Molecules
CELL ADHESION MOLECULES
Type Cell-Matrix Cell-Cell
Integrin * √ √
Homophilic
? N-CAM
? Cadherin
√
Heterophilic √
* Integrins bind to adhesion proteins and some to collagen
After SM Albelda, CA Buck,
FASEB J., 4:2868 (1990)
Schematic of a typical integrin
The RGD* amino acid sequence on
adhesion proteins (e.g., fibronectin) was
identified as the integrin-binding region
(i.e., the ligand for integrin receptors) –
E Ruoslathi and MD Pierschbacher,
Sci., 238:491 (1987)
* arginine-glycine-aspartic acid
Integrin Signaling, FG Giancotti, E Ruoslahti, Sci., 285:1028 (1999)
Image removed due to copyright considerations.
Figure 2. Matrix binding promotes integrin clustering and association with the
cytoskeleton. This in turn promotes further integrin clustering and matrix
organization in a positive feedback system. RGD, Arg-Gly-Asp integrin-binding
motif; Tal, talin; Pax, paxillin; Vin, vinculin; CAS, p130CAS.
Cytoskeletal Component
Proposed Model of Fibroblast Focal Adhesion In Vitro
Image removed due to copyright considerations.
J NIH 5:50 (1993)
Rat Fibroblast on Culture Dish
Fluorescent stains
for actin (green)
and focal adhesion
kinase (red)
Border of the
cell on the
culture dish
Image removed due to copyright considerations.
J NIH 5:49 (1993)
INTEGRINS AND THEIR LIGANDS
β1
αν
α5
α4
α8
α7
α6
α3
α2
α1
α10
β3
β5
β6
αII
β4
β7
Collagen
Laminin
Fibronectin
Fibrinogen
Vitronectin
Adapted from: http://www.scripps.edu/cb/gardner/integrins.htm
INTEGRINS
(from http://life.kjist.ac.kr/htm/lab/cell/integrin/integrin.htm)
Integrins are membrane-bound molecules (receptors) that can bind to
extracellular matrix molecules (“adhesion proteins” and collagen). They are
the principal mechanism by which cells both bind to and respond to the
extracellular matrix. They are part of a large family of cell adhesion
molecules which are involved in cell-extracellular matrix and cell-cell
interactions. Functional integrins consist of two transmembrane glycoprotein
subunits that are non-covalently bound. Those subunits are called alpha and
beta. The alpha subunits all have some homology to each other, as do the beta
subunits. The receptors always contain one alpha chain and one beta chain
and are thus called heterodimeric. Both of the subunits contribute to the
binding of ligand. Until now 16 alpha and 8 beta subunits have been
identified. From these subunits some 22 integrins are formed in nature, which
implicates that not all possible combinations exist. The beta-4 subunit for
instance can only form a heterodimer with the alpha-6 subunit. On the other
hand the beta-1 subunit can form heterodimers with ten different alpha
subunits. Because not all the beta-1 alpha heterodimers have the same ligand
specificities, it is believed that the alpha chain is at least partly involved in the
ligand specificity.
INTEGRINS
(from http://life.kjist.ac.kr/htm/lab/cell/integrin/integrin.htm)
Integrins differ from other cell-surface receptors in that they bind their
ligands with a low affinity (106-109 liters/mole) and that they are usually
present at 10-100 fold higher concentration on the cell surface. The
integrins however can only bind their ligands when they exceed a certain
minimal number of integrins at certain places, called focal contacts and
hemidesmosomes. So when the integrins are diffusely distributed over
the cell surface, no adhesion will be present, but when after a certain
stimuli these integrins cluster for example in focal contacts their
combined weak affinities give rise to a spot on the cell surface which has
enough adhesive (sticking) capacity to adhere to the extracellular
matrix. This is a very useful situation, because in this way cells can bind
simultaneously but weakly to large numbers of matrix molecules and
still have the opportunity to explore their environment without losing all
attachment to it by building or breaking down focal contacts. If the
receptors were to bind strongly to their ligands, cells would probably be
irreversibly bound to the matrix, depriving them from motility. This
problem does not arise when attachment depends on multiple weak
adhesions.
INTEGRINS
(from http://life.kjist.ac.kr/htm/lab/cell/integrin/integrin.htm)
Integrins can bind to an array of ligands. Common ligands are
fibronectin and laminin, which are both part of the CT
extracellular matrix and basal lamina. Both of these ligands
mentioned above are recognized by multiple integrins. For
adhesion to ligands both integrin subunits are needed, as is the
presence of cations. The alpha chain has cation binding sites.
Integrins are composed of long extracellular domains which
adhere to their ligands, and short cytoplasmic domains that
link the receptors to the cytoskeleton of the cell.
The structure of alpha subunits is very similar. All contain 7
homologous repeats of 30-40 amino acids in their extracellular
domain, spaced by stretches of 20-30 amino acids. The three or
four repeats that are most extracellular, contain sequences
with cation-binding properties. These sequences are thought to
be involved in the binding of ligands, because the interaction of
integrins with their ligand is cation-dependent.
Integrin Signaling, FG Giancotti, E Ruoslahti, Sci., 285:1028 (1999)
Image removed due to copyright considerations.
Figure 1. Cell survival and cell proliferation require interaction with the extracellular
matrix. (A) Epithelial cells in some tissues, such as skin and gut, are continuously renewed
from stem cells that rest on a basement membrane. Neighboring cells migrate into the
space left empty by cells that have moved away to differentiate. (B) Certain epithelia, such
as those of the mammary gland and prostate, are not continuously renewed. In this case,
interaction with the matrix appears to promote differentiation. During involution, the
basement membrane is dissolved by proteolysis, and the cells undergo apoptosis.
Integrin Signaling, FG Giancotti,
E Ruoslahti, Sci., 285:1028 (1999)
Figure 4. Major signaling pathways
that are known (solid arrows) or
presumed (dashed arrows) to be
coordinately regulated by integrins
and growth factors receptors. These
pathways control immediate-early
gene expression, the cell cycle
machinery, and cell survival.
Image removed due to copyright considerations.