TABLE 302-a—1
TABLE 302-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
2
Notes assume that extract is examined by GLC with columns at 200 C and, at a minimum, halogen-selective detector (DG3
or 16) and phosphorus-selective detectors (DG2 or 14 or 19). Notes indicate those chemicals that can be determined only by
use of columns, temperatures, and/or detectors other than the minimal ones.
Table 302-a: Recovery of Chemicals Through Method 302 (E1-E3 + DG1-DG19)
(acetone extraction, partitioning or Hydromatrix removal of water, GLC determination
with various columns and detectors)
Chemical Recovery
1
Notes
2
(4-chlorophenyl)-urea NR
1,2,4,5-tetrachloro-3-(methylthio)benzene R
1,2,4-triazole V N detector required.
2,3,5-trimethacarb C N detector required.
2,4,5-trichloro-alpha-methylbenzenemethanol R
2,4-dichloro-6-nitrobenzenamine C (110%) n=1
2,6-dichlorobenzamide C
2-chloroethyl myristate C
2-methoxy-3,5,6-trichloropyridine C Low temperature column recommended.
3,4,5-trimethacarb C N detector required.
3,4-dichloroaniline V (44-84%)
3,5-dichloroaniline S (15-62%)
3-(3,4-dichlorophenyl)-1-methoxyurea R GLC not reliable for quantitation.
3-carboxy-5-ethoxy-1,2,4-thiadiazole NR
3-chloro-5-methyl-4-nitro-1H-pyrazole C OV-101 peak tails severely.
3-ketocarbofuran S (0-150%)
3-methyl-4-nitrophenol V (65-153%) Interferences from sample extract may have
caused variable results.
4'-hydroxy bifenthrin C High temperature GLC column required.
4-(dichloroacetyl)-1-oxa-4-azapiro[4.5]decane C Low level (0.05 ppm) fortification in corn grain
obscured by matrix.
4-(phenylamino)phenol C
4-chlorobenzenamine S (23-43%)
4-chlorophenoxyaniline S (10-29%) Poor EC detector sensitivity; halogen-selective
detector required.
6-benzyladenine C N detector required.
acephate C Wide bore or DEGS column required.
acetochlor C
acrinathrin V (80-136%)
TABLE 302-a
Pesticide Analytical Manual Vol. I
TABLE 302-a—2
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
alachlor C
aldrin C
allidochlor C Low temperature DEGS column used.
alpha-cypermethrin C
ametryn C N or S detector required.
aminocarb C N detector required.
amitraz S (0-70%) N detector, high temperature column required.
anilazine V GLC response variable.
aramite C
atrazine C
azinphos-ethyl C
azinphos-methyl C DEGS column unsuitable.
azinphos-methyl oxygen analog C
bendiocarb C N detector required.
benfluralin C
benodanil C
benoxacor C
bensulide C Results may be variable with certain GLC systems.
benzoylprop-ethyl P (79%)
BF 490-1 C
BF 490-2 C
BF 490-9 C
BHC, alpha- C
BHC, beta- C
BHC, delta- C
bifenox C
bifenthrin V ( 66-133%)
binapacryl C N detector required.
biphenyl C FID required.
bitertanol C GLC with high temperature column, N/P detec-
tor required.
bromacil C
bromophos C
bromophos-ethyl C
TABLE 302-a—3
TABLE 302-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
bromopropylate C
bromuconazole C
BTS 27919 C N detector required.
Bulan C
bupirimate C N or S detector required.
butachlor C
butralin V (77-90%) N detector required.
butylate V (73-99%) N detector required.
cadusafos C
captafol C
captan C
carbaryl C N detector required for GLC determination;
GLC not the method of choice.
carbetamide C N detector required.
carbofuran C N detector required for GLC determination;
GLC not the method of choice.
carbophenothion C
carbophenothion oxygen analog C
carbophenothion sulfone C
carbosulfan P (47-75%) N or S detector required.
carboxin C N or S detector required.
CGA 100255 S (37-146%) N detector required, but response is poor.
CGA 118244 V
CGA 14128 C
CGA 150829 V (40-111%) N detector required; wide bore or DEGS column
recommended.
CGA 171683 C N detector, wide bore or DEGS column required.
CGA 37734 C N detector required but response variable.
CGA 91305 V
CGA 94689A V (44-108%) N detector required.
CGA 94689B S (39-94%) N detector required, but response varies widely
with different columns.
CGA-232449 C Needs N detector.
chlorbenside C
chlorbromuron V (73-100%)
TABLE 302-a
Pesticide Analytical Manual Vol. I
TABLE 302-a—4
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
chlorbufam C
chlordane C
chlordane, cis- C
chlordane, trans- C
chlordimeform hydrochloride P (80%)
chlorethoxyfos V (64-119%) Recoveries performed with electron capture de-
tector.
chlorfenapyr P (73-82%)
chlorfenvinphos, alpha- C
chlorfenvinphos, beta- C
chlorflurecol methyl ester C
chlorimuron ethyl ester P (69-70%)
chlormephos C Low temperature column required.
chlornitrofen C (80%)
chlorobenzilate C
chloroneb C Low temperature column required.
chloropropylate P (64%)
chlorothalonil S Recovery may be 0%.
chlorothalonil trichloro impurity R
chloroxuron C
chlorpropham C
chlorpyrifos C
chlorpyrifos oxygen analog C Wide bore or DEGS column recommended.
chlorpyrifos-methyl C
chlorthiophos C
chlorthiophos oxygen analog C
chlorthiophos sulfone C
chlorthiophos sulfoxide C
clodinafop-propargyl V Recovery test yielded very high recoveries
(>200%) from wheat.
clofentezine R Degrades on GLC in presence of extract.
clomazone C
cloquintocet-mexyl V (57-137%)
coumaphos C
TABLE 302-a—5
TABLE 302-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
coumaphos oxygen analog C
CP 51214 C
crotoxyphos C
crufomate C
cyanazine C
cyanofenphos C
cyanophos C
cycloate C N or S detector required.
cycluron C N detector required.
cyfluthrin C High temperature column required.
cymoxanil V (70-107%) N detector required; GLC rrts and responses
variable.
cypermethrin C
cyprazine C
cyproconazole C
cyprodinil C Needs N detector
cyromazine S (16-20%)
dazomet S (<10%)
DCPA C
DDE, o,p'- C
DDE, p,p'- C
DDT, o,p'- C
DDT, p,p'- C
deltamethrin C
demeton-O C
demeton-O sulfone C
demeton-O sulfoxide C
demeton-S C
demeton-S sulfone C Wide bore or DEGS column recommended.
demeton-S sulfoxide C Wide bore or DEGS column required.
des N-isopropyl isofenphos C
desisopropyl iprodione P (67-84%)
desmethyl norflurazon V (63-200%)
di-allate C
TABLE 302-a
Pesticide Analytical Manual Vol. I
TABLE 302-a—6
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
dialifor C
diazinon C
diazinon oxygen analog C (80%)
dichlobenil C Low temperature column required.
dichlofenthion C
dichlofluanid C
dichlone P (55%) May break down.
dichlorvos C Low temperature column required; wide bore or
DEGS recommended.
diclobutrazol C Wide bore column recommended.
diclofop-methyl C
dicloran C
dicofol, o,p'- C
dicofol, p,p'- C
dicrotophos C Wide bore or DEGS column required.
dieldrin C
diethatyl-ethyl C
difenoxuron R 79-95% recovered at 1 and 5.5 ppm, but subject
to interferences.
dimethachlor C
dimethametryn C N or S detector required.
dimethipin C
dimethoate C Wide bore or DEGS column recommended.
dimethomorph (prop) V (87-133%) High temperature column required.
dinitramine C N detector required.
dinobuton C
dinocap C N detector required.
dioxabenzofos C
dioxacarb C N detector required; used Megabore Carbowax
column.
dioxathion V (72-94%)
diphenamid V (57-155%) N detector required.
diphenyl 2-ethylhexyl phosphate C mean recovery 104.2%, n=15
diphenylamine C N detector required.
disulfoton C
TABLE 302-a—7
TABLE 302-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
disulfoton sulfone C
disulfoton sulfoxide C Wide bore or DEGS column required.
dithianon NR Breaks down in presence of extract.
diuron C Low temperature column required.
edifenphos C High recovery (113-121%) reported.
endosulfan I C
endosulfan II C
endosulfan sulfate C
endrin C
endrin aldehyde C
EPN C
esfenvalerate C High temperature column required.
etaconazole C Wide bore column recommended.
ethalfluralin C
ethephon NR
ethiofencarb C N or S detector required; responses variable.
ethiolate C Low temperature column, N or S detector re-
quired.
ethion C
ethion oxygen analog C
ethirimol P (73%)
ethofumesate C S selective detector required.
ethoprop C
ethoxyquin C N detector required.
ethyl p-toluene sulfonamide C N or S detector required.
ethylenethiourea S (0-48%) Short, low temperature DEGS or wide bore col-
umn, N or S detector required.
etridiazole C Low temperature column recommended.
etrimfos C
etrimfos oxygen analog C
famphur C
famphur oxygen analog C Quantitation affected by poor GLC.
fenamiphos C
fenamiphos sulfone C
TABLE 302-a
Pesticide Analytical Manual Vol. I
TABLE 302-a—8
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
fenamiphos sulfoxide C
fenarimol C
fenarimol metabolite B NR
fenarimol metabolite C S (6%)
fenbuconazole C
fenfuram C
fenitrothion C
fenitrothion oxygen analog C
fenoxaprop ethyl ester S (0-40%)
fenoxycarb C N detector required.
fenpropimorph C N detector required.
fensulfothion C
fensulfothion oxygen analog C
fensulfothion sulfone C
fenthion C
fenthion oxygen analog C
fenthion oxygen analog sulfoxide C
fenthion sulfone C
fenvalerate C High temperature column required.
fipronil S (0-72%) Corn forage sample interfered with determina-
tion.
flamprop-M-isopropyl C
flamprop-methyl C
fluazifop butyl ester C (78-112%)
fluchloralin C
flucythrinate C High temperature column required.
fludioxonil V (49-121%) Requires N detector.
flusilazole C Wide bore column recommended.
fluvalinate C High temperature column required.
FOE 5043 C
folpet C
fonofos C
fonofos oxygen analog V (57-108%)
formothion C
TABLE 302-a—9
TABLE 302-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
fosthiazate C
fuberidazole C May break down in solution. Temp program
separated from interference in tomato.
furilazole C
G-27550 C N detector required.
Gardona C
heptachlor C
heptachlor epoxide C
heptenophos C
hexachlorobenzene C
hexaconazole C
hexazinone P (57-76%) N detector required; high temperature column
may be needed.
imazalil C Wide bore column recommended.
imazamethabenz methyl ester C N detector required, though halogen-selective
detector may respond.
IN-A3928 S (23-39%)
IN-B2838 P (75-84%)
IN-T3935 S (20%)
IN-T3936 S (29-34%)
IN-T3937 S (25%) N detector required.
iprobenfos C
iprodione C
iprodione metabolite isomer C
isazofos C
isocarbamid C
isofenphos C
isofenphos oxygen analog C
isopropalin C N detector required.
isoprothiolane C
isoproturon S (44-67%) GLC poor; requires wide bore column; com-
pound may degrade.
isoxaben C N detector required.
isoxaflutole NR Crop interference may have prevented measure-
ment of recovery.
TABLE 302-a
Pesticide Analytical Manual Vol. I
TABLE 302-a—10
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
jodfenphos C
kresoxim-methyl P (73-89%)
KWG 1323 C
lambda-cyhalothrin C
lenacil C N detector required.
leptophos C
leptophos oxygen analog C
leptophos photoproduct C
lindane C
linuron V (57-101%)
malathion C
malathion oxygen analog C
MB 46513 C
MB45950 S (0-35%)
MB46136 S (0-19%)
mecarbam C
mefluidide R 123% recovered of 3 ppm added; subject to
interference, poor GLC.
melamine NR
mephosfolan C
metalaxyl C N detector required but response variable.
metasystox thiol C
metazachlor C
methabenzthiazuron C (85-86%)
methamidophos V For complete recovery, use variation from PAM I
302 E5/E6
methidathion C
methiocarb C N or S detector required for GLC determination;
GLC not the method of choice.
methiocarb sulfone S Some reports of no recovery; N or S detector
required.
methiocarb sulfoxide P (60-80%) GLC not preferred, requires N or S detector,
wide bore or DEGS column.
methoprotryne C Wide bore column recommended.
methoxychlor olefin C
TABLE 302-a—11
TABLE 302-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
methoxychlor, p, p'- C
methyl 4-chloro-1H-indole-3-acetate R
metobromuron C
metolachlor C
metolcarb C N detector, wide bore, DEGS, or low tempera-
ture OV-17 column required.
metoxuron V (73-110%) Requires low temperature column.
metribuzin V N or S detector required.
metribuzin, deaminated diketo metabolite NR
metribuzin, deaminated metabolite C N or S detector required.
metribuzin, diketo metabolite NR
mevinphos, (E)- C Wide bore or DEGS column required for separa-
tion from (Z)-.
mevinphos, (Z)- C Wide bore or DEGS column required for separa-
tion from (E)-.
MGK 264 C
mirex P (71-83%)
molinate C Recovery tested at 0.053 and 0.264 ppm.
monocrotophos C Response enhanced by co-extractives. Wide bore
or DEGS column required.
monolinuron C
myclobutanil C Wide bore column recommended.
myclobutanil alcohol metabolite S (30-55%) Poor N/P detector sensitivity.
myclobutanil dihydroxy metabolite NR
N, N-diallyl dichloroacetamide C
naled C May break down to dichlorvos on GLC column.
Wide bore or DEGS column required.
napropamide C N detector, wide bore or DEGS column required.
neburon C
nitralin C N or S detector required.
nitrapyrin C
nitrofen C
nitrofluorfen C
nitrothal-isopropyl C N detector required.
nonachlor, cis- C
TABLE 302-a
Pesticide Analytical Manual Vol. I
TABLE 302-a—12
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
nonachlor, trans- C
norea C N detector required.
norflurazon V (60-200%)
nuarimol C
octachlor epoxide C
octhilinone C N or S detector required.
ofurace C
omethoate C Wide bore or DEGS column required.
ovex C
oxadiazon C
oxadixyl C N detector required.
oxamyl oxime metabolite C Lower temperature column needed to separate
from coextractives.
oxycarboxin R Matrix enhancement of response causes high
results.
oxydemeton-methyl C Wide bore or DEGS column required.
oxydemeton-methyl sulfone C Wide bore or DEGS column required; poor GLC
makes quantitation questionable.
oxyfluorfen C Poor N/P detector sensitivity.
oxythioquinox C N or S detector required; wide bore or short
DEGS column recommended.
paclobutrazol C Wide bore column recommended.
parathion C
parathion oxygen analog C
parathion-methyl C
PB-9 V (106-215%)
pebulate C
penconazole C Wide bore column recommended.
pendimethalin C N detector required.
pentachloroaniline C
pentachlorobenzene C
pentachlorobenzonitrile C
pentachlorophenyl methyl ether C
pentachlorophenyl methyl sulfide C
permethrin, cis- C High temperature column recommended.
TABLE 302-a—13
TABLE 302-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
permethrin, trans- C High temperature column recommended.
Perthane C
phenthoate C
phenylphenol, o- C FID required.
phorate C
phorate oxygen analog C
phorate oxygen analog sulfone C
phorate oxygen analog sulfoxide C GLC retention times and responses variable.
phorate sulfone C GLC variable.
phorate sulfoxide C GLC retention times and responses variable.
phosalone C
phosalone oxygen analog C Poor GLC detector sensitivity.
phosfolan C
phosmet C
phosphamidon C
phoxim C Low temperature column required; degrades at
200 .
phoxim oxygen analog C
piperophos C
pirimicarb C N detector required.
pirimiphos-ethyl C
pirimiphos-ethyl oxygen analog C
pirimiphos-methyl C
pretilachlor C
probenazole C N or S detector required; FPD-S more sensitive
than N/P.
prochloraz C High temperature column required.
procyazine C
procymidone C
prodiamine C Recoveries of 0.5 and 1 ppm from apples: 110,
125%, respectively.
profenofos C
profluralin V (40-90%)
Prolan P (58%)
TABLE 302-a
Pesticide Analytical Manual Vol. I
TABLE 302-a—14
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
promecarb V N detector required; GLC not determinative step
of choice.
prometryn C N or S detector required.
pronamide C
propachlor C
propanil C
propargite C S detector required.
propazine C
propetamphos C
propham C N detector required; low temperature column
recommended.
propiconazole C Wide bore column recommended.
propoxur C N detector required for GLC.
prothiofos C
prothoate C
PYPAC V (144-162%) Low temperature column, N detector required.
pyracarbolid C N detector required.
pyrazon C Wide bore column recommended.
pyrazophos C
pyridaphenthion C S detector is less sensitive than FPD or N/P.
pyrimethanil C
pyriproxyfen C N detector required.
quinalphos C
quintozene C
quizalofop ethyl ester C Wide bore column recommended.
RH-6467 S (0-17%)
RH-9129 V (68-92%)
RH-9130 P (48-71%)
ronnel C
ronnel oxygen analog C
RPA202248 NR
schradan C
SDS-67131 C
simazine C
TABLE 302-a—15
TABLE 302-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
simetryn C N or S detector required.
sulfallate C
sulfanilamide NR
sulfotep C Wide bore or DEGS column recommended.
Sulphenone C
sulprofos C
sulprofos oxygen analog sulfone C
sulprofos sulfone C
sulprofos sulfoxide C
TCMTB C
TDE, p,p'- C
TDE, p,p'-, olefin C
tebuconazole C
tecnazene C
tefluthrin C Recovery tested at 0.275 and 1.374 ppm.
TEPP C
terbacil C
terbufos C
terbufos oxygen analog C
terbufos oxygen analog sulfone C
terbufos sulfone C
terbumeton C Recoveries of 0.5 and 1 ppm from apples: about
120%.
terbuthylazine C
terbutryn C N or S detector required.
tetradifon C
tetramethrin C
tetrasul C
thiabendazole C N or S detector required for GC determination.
thiazopyr C Recovery at 0.5 ppm; interferences prevented
measurement at 0.1 ppm.
thiobencarb C
thiometon C Degrades while standing in extract.
thionazin C
TABLE 302-a
Pesticide Analytical Manual Vol. I
TABLE 302-a—16
Chemical Recovery
1
Notes
2
Table 302-a: Recovery Through 302 (E1-E3 + DG1-DG19)
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
THPI C N detector, wide bore or DEGS column required.
tolylfluanid C
toxaphene C
tralkoxydim V (38-106%) Recoveries of two OV-101 peaks are different
from one another.
tralomethrin C
tri-allate C
triadimefon C Wide bore column recommended.
triadimenol C Wide bore column recommended.
triazamate C
triazophos C
tribufos C
trichlorfon C Often converts to dichlorvos on GLC column.
Wide bore or DEGS column required.
tricyclazole C N or S detector required; wide bore column
recommended.
tridiphane C
trietazine C Recovery tested at 0.11 and 0.55 ppm.
triflumizole C Wide bore column recommended.
trifluralin C
triflusulfuron methyl ester V (67-106%)
triphenyl phosphate C
tris(2-ethylhexyl) phosphate C (68-112%) mean recovery 97.6%, n=11
tris(beta-chloroethyl) phosphate C
tris(chloropropyl) phosphate C
Tycor C May break down in solution. Temp program
separated from interference in tomato.
vamidothion sulfone C
vinclozolin C
vinclozolin metabolite B C Severely subject to influence of matrix; levels
<1.0 ppm had very high recovery.
vinclozolin metabolite E C Severely subject to influence of matrix; levels
<1.0 ppm had very high recovery.
vinclozolin metabolite F R Poor chromatography, influence of matrix pre-
vent quantitation of recovery.
vinclozolin metabolite S V (59-137%)
TABLE 302-b–1
TABLE 302-b
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
2
Recovery results refer to complete method; blank entry in this column indicates Florisil elution was tested but not complete
method.
3
Eluants(s) in which chemical is eluted from Florisil, according to directions in 302 C5, i.e., 15 and 50% ethyl ether/petroleum
ether (EE/PE). Entries for chemicals not recovered indicate which eluants were used in tests.
4
“Florisil only” refers to tests in which elution patterns were tested by added reference standard solutions directly to Florisil
column.
Table 302-b: Recovery of Chemicals Through Method 302 (E1-E3 + C5 + DG1-DG19)
(acetone extraction, partitioning or Hydromatrix removal of water, Florisil column
cleanup, GLC determination with various columns and detectors)
Chemical Recovery
1,2
Eluant, C5
3
Notes
4
2,3-dihydro-3,3-methyl-2-oxo=
-5-benzofuranyl methyl sulfonate NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
2,6-dichlorobenzamide NR 50 83% elution from Florisil only in 200 mL
ethyl ether.
2-hydroxy-2,3-dihydro-3,3-methyl=
-5-benzofuranyl methyl sulfonate NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
2-methoxy-3,5,6-trichloropyridine P (65%) 15 Low temperature column recommended.
acetochlor C 15+50 80% eluted in 15% EE/PE.
alachlor P (68%) 15
aldrin C 15
allethrin C (80%) 15
atrazine C 15+50 Eluted in 50% EE/PE.
azinphos-ethyl C 15
BHC, alpha- C 15
BHC, beta- C 15
BHC, delta- C 15
bifenthrin C 15
binapacryl C (83%) 15
bioresmethrin NR 15 Some elution from Florisil in 200 mL 50%
EE/PE, more in 200 mL 75% EE/PE.
biphenyl C 15 FID required.
bromophos C 15
bromopropylate C (80%) 50
bupirimate S (10-30%) 15
captafol NR 15 Some elution from Florisil in 50% EE/PE
after 15%.
TABLE 302-b
Pesticide Analytical Manual Vol. I
TABLE 302-b–2
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
Chemical Recovery
1,2
Eluant, C5
3
Notes
4
Table 302-b: Recovery Through 302 (E1-E3 + C5 + DG1-DG19)
captan S (25%) 15 Additional elution from Florisil in 50% EE/
PE after 15%.
carfentrazone ethyl ester C 15 Some additional elution in 50% EE/PE
possible.
chlorbenside C 15
chlordane C 15
chlordane, cis- C 15
chlordane, trans- C 15
chlordimeform hydrochloride NR 15-50
chlorfenapyr C 15+50
chlorflurecol methyl ester NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
chlornitrofen C 15
chlorothalonil C (81%) 15 Additional elution from Florisil in 200 mL
50% EE/PE.
chlorpyrifos C 15
chlorpyrifos oxygen analog NR 15 Elution from Florisil with 50% EE/PE not
tested.
chlorpyrifos-methyl C 15
chlorthiophos oxygen analog NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
chlorthiophos sulfone S (8%) 15
chlorthiophos sulfoxide NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
clofentezine S (27-50%) 15 Complete elution from Florisil only; de-
grades on GLC in presence of extract.
cymiazole NR 6-15-50 No elution from Florisil only in 6, 15, or 50%
EE/PE or 100% EE.
cypermethrin C 15
DCPA C 15
DDE, o,p'- C 15
DDE, p,p'- C 15
DDT, o,p'- C 15
DDT, p,p'- C 15
deltamethrin C 15 Very poor EC detector sensitivity.
diazinon C 15
dichlofluanid C 15+50 60% eluted in 15% EE/PE
TABLE 302-b–3
TABLE 302-b
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
Chemical Recovery
1,2
Eluant, C5
3
Notes
4
Table 302-b: Recovery Through 302 (E1-E3 + C5 + DG1-DG19)
diclobutrazol P 15 56-70% elution from Florisil only; 50% not
tested; wide bore column recommended.
dicloran C 15
dicofol, o,p'- C 15
dicofol, p,p'- C 15 May be variable.
dieldrin C 15
difenoxuron NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
dimethachlor NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
dinobuton C (83%) 15
endosulfan I C 15
endosulfan II C 15
endosulfan sulfate C 15 Additional elution possible in 50% EE/PE.
endrin C 15
esfenvalerate C 15
etaconazole S 15 30% elution from Florisil only; 50% not
tested; wide bore column recommended.
fenfuram P (45%) 15 Partial recovery also when Florisil eluted with
MeCl2 eluant #3.
fenpropathrin C 15
fenson C 15
fensulfothion sulfone NR 50 No elution from Florisil only in 50% EE/PE.
fenvalerate C 15
flamprop-M-isopropyl NR 15 Complete elution from Florisil only in 50%
EE/PE plus additional EE.
flamprop-methyl NR 6-15-50 No elution from Florisil only in 6, 15, or 50%
EE/PE; complete elution with EE.
fluchloralin C 15
flucythrinate C 15+50 About 80% eluted in 15% EE/PE
flusilazole S 15 35-44% elution from Florisil only; wide bore
column recommended.
fluvalinate C 15
FOE 5043 thioglycolate sulfoxide NR not eluted from Florisil
folpet C 15+50 78% eluted in 15% EE/PE
fonofos C 15
TABLE 302-b
Pesticide Analytical Manual Vol. I
TABLE 302-b–4
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
Chemical Recovery
1,2
Eluant, C5
3
Notes
4
Table 302-b: Recovery Through 302 (E1-E3 + C5 + DG1-DG19)
fuberidazole NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
heptachlor C 15 Elution from Florisil not always complete.
heptachlor epoxide C 15
hexachlorobenzene C 15 Recovery test run with 6,15,50% EE/PE,
elution in 6%.
hexaconazole NR 15
imazalil NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
iprodione S (24-97%) 15 Complete elution from Florisil requires more
polar eluants.
isocarbamid NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
lindane C 15
metalaxyl NR 15
methabenzthiazuron NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
methoprotryne NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
methyl 3,5-dichlorobenzoate C 15
mirex C 15
myclobutanil NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
nitrapyrin C 15
nitrofen C 15
nitrothal-isopropyl C 15
nonachlor, trans- C 15
norflurazon NR 15 Elution from Florisil with 50% EE/PE not
tested.
ovex C 15
oxadiazon C 15
oxythioquinox C (79-96%) 15
paclobutrazol P 15 44-55% elution from Florisil only; 50% not
tested; wide bore column recommended.
parathion C 15
parathion-methyl C 15
TABLE 302-b–5
TABLE 302-b
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
Chemical Recovery
1,2
Eluant, C5
3
Notes
4
Table 302-b: Recovery Through 302 (E1-E3 + C5 + DG1-DG19)
penconazole S 15 42-54% elution from Florisil only; 50% not
tested; wide bore column recommended.
pentachloroaniline C 15 Recovery test run with 6,15,50% EE/PE,
elution in 6%.
pentachlorobenzene C 15 Recovery test run with 6,15,50% EE/PE,
elution in 6%.
pentachlorophenyl methyl sulfide C 15 Recovery test run with 6,15,50% EE/PE,
elution in 6%.
permethrin, cis- C 15 High temperature column recommended.
permethrin, trans- C 15 High temperature column recommended.
phenmedipham NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
phenothrin P (60%) 15 No additional elution in 200 mL 50% EE/
PE; wide bore column recommended.
phenylphenol, o- C 15 FID required.
phorate sulfoxide NR 15-50 Recovery test included elution from Florisil
with 50% EE/PE only.
pirimicarb S (25%) 15
pretilachlor 15 Elution from Florisil only complete in 15%
EE/PE.
procymidone C 15
prodiamine 15 Elution from Florisil only complete in 15%
EE/PE.
propargite C 15
propham C 15 N detector required; low temperature
column recommended.
propiconazole P 15 46-50% elution from Florisil only; 50% not
tested; wide bore column recommended.
pyrethrins C 15+50 Most eluted in 15% EE/PE.
quintozene C 15
quizalofop ethyl ester C 15
simazine P (69%) 15
TDE, p,p'- C 15
tebufenozide NR 15
TEPP NR 15
terbumeton NR 15-50 No elution from Florisil only in 15 or 50%
EE/PE.
terbuthylazine C 15
TABLE 302-b
Pesticide Analytical Manual Vol. I
TABLE 302-b–6
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)
Chemical Recovery
1,2
Eluant, C5
3
Notes
4
Table 302-b: Recovery Through 302 (E1-E3 + C5 + DG1-DG19)
tetradifon C 15
thiometon C 15
THPI NR 15-50 Only small amount recovered (5%) in
subsequent elution with 200 mL EE .
toxaphene C 15
tralomethrin C 15
triadimefon S (7%) 15 72-84% elution from Florisil only; 50% not
tested; wide bore column recommended.
triadimenol S 15 40-45% elution from Florisil only; 50% not
tested; wide bore column recommended.
tricyclazole NR 15 No elution from Florisil only in 15% EE/PE;
50% not tested.
triflumizole P 15 46-52% elution from Florisil only; 50% not
tested; wide bore column recommended.
vinclozolin C 15
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92) TABLE 302-c–1
TABLE 302-c
Pesticide Analytical Manual Vol. I
Table 302-c: Recovery of Chemicals Through Method 302 (E1-E3 + C3 + DL1)
(acetone extraction, partitioning or Hydromatrix removal of water, charcoal/silanized
Celite column cleanup, HPLC with post-column hydrolysis and derivatization,
fluorescence detection)
Chemical Recovery
1
Rrt
2
ng
3
Notes
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
2
Retention time, relative to carbofuran, on C-8 column described in DL1.
3
ng that cause 50% full scale deflection detector response in DL1.
2,3,5-trimethacarb C
3,4,5-trimethacarb C
3-hydroxycarbofuran C 0.6 10 mean recovery 97.6%, n=45
aldicarb C 0.83 14 mean recovery 89.2%, n=210
aldicarb sulfoxide C 0.33 9 mean recovery 98.6%, n=108
aldoxycarb C 0.4 9 mean recovery 102%, n=111
aminocarb C
bufencarb C 1.44 19 Major peak is listed. mean recovery 97.4%,
n=27
butocarboxim S (0-108%) 0.75 15 mean recovery 56.1%, n=22
carbaryl C 1.06 7 mean recovery 98.1%, n=147
carbofuran C 1 10 mean recovery 97.4%, n=121
dioxacarb P (72%) 0.67 15
isoprocarb C 1.13 8
methiocarb C 1.26 10 mean recovery 99.9%, n=67
methomyl C 0.46 10 mean recovery 94.1%, n=128
metolcarb C 0.85 10 mean recovery 90.7%, n=12
oxamyl C 0.44 10 mean recovery 94.3%, n=41
promecarb C 1.31 10 mean recovery 99.9%, n=29
propoxur C 0.98 8 mean recovery 92.2%, n=48
XMC C 1.06 10 mean recovery 95.6%, n=28
TABLE 302-c
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)TABLE 302-c–2
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92) TABLE 302-d–1
TABLE 302-d
Pesticide Analytical Manual Vol. I
Table 302-d: Recovery of Chemicals Through Method 302 (E2/E3 + C1 + DG1-DG19)
(acetone extraction, Hydromatrix removal of water, Florisil cleanup with one methylene
chloride eluant, GLC determination with various columns and detectors)
Chemical Recovery
1
Notes
2
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
2
Notes assume that extract is examined by GLC with columns at 200 C and, at a minimum, halogen-selective detector (DG3
or 16) and phosphorus-selective detectors (DG2 or 14 or 19). Notes indicate those chemicals that can be determined only by
use of columns, temperatures, and/or detectors other than the minimal ones.
1,2,3,5-tetrachlorobenzene V (63-141%) mean recovery 91.6%, n=16
2,3,5,6-tetrachloroaniline C (67-110%%) mean recovery 85.5%, n=10
2,4-dichloro-6-nitrobenzenamine V (65-123%) mean 91%, n=7
aldrin C mean recovery 86.9%, n=16
allethrin C mean recovery 91.8%, n=4
alpha-cypermethrin C mean recovery 98.0%, n=15
azafenidin V (45-160%) High Temperature column required.
BHC, alpha- V (68-89.5%) mean recovery 79.0%, n=2
bifenthrin C (59-110%) mean recovery 91.4%, n=15
bromopropylate NR
butachlor C mean recovery 91.7%, n=2
captafol C mean recovery 101.7%, n=4
captan V (0-139%) mean recovery 65.2%, n=20
carbaryl C recovery 107%, n=1
chlordane P (64%) mean recovery 64.4%, n=2
chlordane, cis- C
chlordane, trans- C
chlorobenzilate NR mean recovery 5.5%, n=11
chlorothalonil S (0-93%) mean recovery 36.9%, n=17
chlorpropham V (76-95%) mean recovery 86.1%, n=2
chlorpyrifos C mean recovery 88.9%, n=27
chlorpyrifos-methyl V (54-116%) mean recovery 80.6%, n=16
clodinafop-propargyl V (56-104%)
cloquintocet-mexyl NR Not eluted from Florisil only.
cyfluthrin V (60-117%) mean recovery 86.5%, n=15
cypermethrin C
DCPA P mean recovery 77.0%, n=4
DDE, o,p'- C
TABLE 302-d
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)TABLE 302-d–2
Table 302-d: Recovery Through 302 (E2/E3 + C1 + DG1-DG19)
Chemical Recovery
1
Notes
2
DDE, p,p'- C mean recovery 83.7%, n=6
DDT, o,p'- V (58-111%) mean recovery 86.3%, n=15
DDT, p,p'- C mean recovery 91.4%, n=2
deltamethrin C mean recovery 88.5%, n=5
diazinon C
dichlone P recovery 58.6%, n=1
diclofop-methyl V (56-135%) mean recovery 88.2%, n=29
dicloran V (57-118%) mean recovery 82.1%, n=14
dicofol, o,p'- C
dicofol, p,p'- C mean recovery 107%, n=4
dieldrin C mean recovery 88.4%, n=146
endosulfan I V (64-89%) mean recovery 76.2%, n=4
endosulfan II C mean recovery 93.6%, n=4
endosulfan sulfate C mean recovery 91.9%, n=28
endrin C mean recovery 99.1%, n=2
esfenvalerate V (70-138%) mean recovery 95.9%, n=16
fenarimol S (0-33%) mean recovery 13%, n=21
fenhexamid NR Not recovered from Florisil only.
fenoxaprop ethyl ester C mean recovery 97.8%, n=2
fenvalerate V (65-162%) mean recovery 93.8%, n=21
fluchloralin C mean recovery 91.8%, n=16
fluvalinate C (64-113%) mean recovery 93.4%, n=10
folpet C mean recovery 120%, n=4
haloxyfop methyl ester C recovery 126%, n=1
heptachlor C mean recovery 81.7%, n=2
heptachlor epoxide V (58-118%) mean recovery 87.3%, n=49
hexachlorobenzene C
hexythiazox V (36-89%)
iprodione S (0-95%) mean recovery 32.4%, n=26. Complete recov-
ery requires 50% EE/PE eluant.
iprodione metabolite isomer V (32-149%) mean recovery 85.0%, n=24
isopropalin C mean recovery 85.8%, n=4
lambda-cyhalothrin C mean recovery 106%, n=16, range 87-133%
lindane C mean recovery 84.7%, n=54
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92) TABLE 302-d–3
TABLE 302-d
Pesticide Analytical Manual Vol. I
Table 302-d: Recovery Through 302 (E2/E3 + C1 + DG1-DG19)
Chemical Recovery
1
Notes
2
linuron C mean recovery 88.3%, n=18
methamidophos C mean recovery 91.2%, n=1
methoxychlor olefin C (63-104%) mean recovery 88.2%, n=10
methoxychlor, o, p'- C 72-123% recoveries, TDS
methoxychlor, p, p'- V (76-130%) mean recovery 100.9%, n=19
metolachlor NR
mirex V (37-110%) mean recovery 79.3%, n=15
nitrapyrin V (69-123%) mean recovery 96.1%, n=2
nonachlor, cis- C
nonachlor, trans- C
nuarimol NR
octachlor epoxide C mean recovery 91.5%, n=23
oxadiazon C mean recovery 89.4%, n=2
oxyfluorfen C
parathion C mean recovery 117%, n=1
pentachloroaniline C
pentachlorobenzene C
pentachlorophenyl methyl ether C mean recovery 89.5%, n=1
pentachlorophenyl methyl sulfide V (49-112%) mean recovery 80.7%, n=15
permethrin, cis- C mean recovery 91.6%, n=4
permethrin, trans- C mean recovery 93.5%, n=4
phenylphenol, o- V (76-129%) FID required; mean recovery 97.6%, n=16
phosalone V (27-116%) mean recovery 75.4%, n=16
procymidone C mean recovery 90.7%, n=3
propanil C mean recovery 100.2%, n=2
propargite V (71-125%) mean recovery 93%, n=23
prothiofos V (36-127%) mean recovery 75.8%, n=21
pyrethrins C mean recovery 83.5%, n=6
quintozene P mean recovery 79.6%, n=14
sulfallate V (39-87%) mean recovery 58.9%, n=5
TDE, o,p'- V (70-145%) mean recovery 97.7%, n=17
TDE, p,p'- C
TDE, p,p'-, olefin V (41-128%) mean recovery 78.4%, n=21
tecnazene C mean recovery 83.2%, n=1
TABLE 302-d
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)TABLE 302-d–4
Table 302-d: Recovery Through 302 (E2/E3 + C1 + DG1-DG19)
Chemical Recovery
1
Notes
2
tetradifon C mean recovery 111%, n=4
thiobencarb C mean recovery 90.4%, n=2
toxaphene C mean recovery 94.1%, n=5
tralomethrin C (67-103%) mean recovery 87.5%, n=11
tridiphane V (54-110%) mean recovery 85.1%, n=16
trifluralin P mean recovery 57.0%, n=3
vinclozolin V (61-109%) mean recovery 86.8%, n=14
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92) TABLE 302-e–1
TABLE 302-e
Pesticide Analytical Manual Vol. I
Table 302-e: Recovery of Chemicals Through Method 302 (E1/E4 + C4 + DL1)
(acetone extraction, partitioning to remove water, C-18 cartridge cleanup, HPLC with
post-column derivatization and fluorescence detection)
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
2
Retention time, relative to carbofuran, on C-8 column described in DL1.
3
ng that cause 50% full scale deflection detector response in DL1.
Chemical Recovery
1
Rrt
2
ng
3
Notes
3-hydroxycarbofuran C 0.6 10 mean recovery 94.7%, n=2
aldicarb C 0.83 14 mean recovery 87.4%, n=8
aldicarb sulfoxide C 0.33 9 mean recovery 89.6%, n=9
aldoxycarb V (70-104%) 0.4 9 mean recovery 88.7%, n=8
bitertanol C GLC with high temperature column, N/P
detector required.
bufencarb C 1.44 19 Major peak is listed. recovery 107%, n=1
carbaryl C 1.06 7 mean recovery 88.9%, n=45
carbofuran C 1 10 mean recovery 96.2%, n=3
dioxacarb C 0.67 15 recovery 91.1%, n=1
methiocarb C 1.26 10 mean recovery 97.9%, n=5
methiocarb sulfoxide S 0.64 12 recovery 42.0%, n=1
methomyl C 0.46 10 mean recovery 96.4%, n=36
oxamyl C 0.44 10 mean recovery 95.8%, n=33
phenylphenol, o- C DL2 required; mean recovery 86.9%, n=8.
piperonyl butoxide C mean recovery 91.8%, n=5
pronamide C
propoxur C 0.98 8 mean recovery 85.2%, n=6
TABLE 302-e
Pesticide Analytical Manual Vol. I
Transmittal No. 96-E1 (9/96)
Form FDA 2905a (6/92)TABLE 302-e–2
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92) TABLE 302-f–1
TABLE 302-f
Pesticide Analytical Manual Vol. I
Table 302-f: Recovery of Chemicals Through Method 302 (E7 + C6 + DG1-DG3,
DG6-DG7, DG10, DG13-DG14, or DG16)
(acetone extraction and solid phase extraction cartridges, liquid-liquid partitioning, SAX
cartridge cleanup, GLC determination)
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
Chemical Recovery
1
Notes
3-hydroxycarbofuran C Determination by DL1.
acephate C
aldicarb C Determination by DL1.
aldicarb sulfoxide C Determination by DL1.
alpha-cypermethrin C
atrazine C
azinphos-methyl C Recoveries tend to be >100%.
BHC, alpha- C
BHC, beta- C Recoveries tend to be >100%.
BHC, delta- C
bitertanol C GLC with high temperature column, N/P
detector required.
carbaryl C Determination by DL1.
carbendazim C Determined by UV detector at 280 nm.
carbofuran C Determination by DL1.
carbophenothion C
chlorothalonil C based on two recoveries
chloroxuron C Determination by DL3.
chlorpropham C
chlorpyrifos C
chlorpyrifos-methyl C
chlorthiophos C
cyanazine C
cyfluthrin C
DCPA C
DDE, o,p'- C
DDE, p,p'- C
DDT, o,p'- C
DDT, p,p'- C
TABLE 302-f
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)TABLE 302-f–2
Table 302-f: Recovery Through 302 (E7 + C6 + DG1-DG3, DG6-DG7, DG10, DG13-
DG14, or DG16)
Chemical Recovery
1
Notes
deltamethrin C
demeton-O C
demeton-S sulfone C Recoveries tend to be >100%.
diazinon C
dichlofluanid C Recoveries tend to be >100%.
dichlorvos P (75%)
dicloran C
dicofol, p,p'- C
dicrotophos C
dieldrin C
dimethoate C
dioxathion C
diphenylamine C N detector required.
disulfoton sulfone C
diuron C Determination by DL3.
endosulfan I C
endosulfan II C
endosulfan sulfate C
endrin C
EPN C
esfenvalerate C
etaconazole C
ethion C
fenamiphos P (79%)
fenarimol V (79, 99%)
fenpropimorph C N detector required
fenthion C
fenuron C Determination by DL3.
fluridone P (65%)
folpet C
hexachlorobenzene P (76%)
imazalil C
iprodione C
lindane C
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92) TABLE 302-f–3
TABLE 302-f
Pesticide Analytical Manual Vol. I
Table 302-f: Recovery Through 302 (E7 + C6 + DG1-DG3, DG6-DG7, DG10, DG13-
DG14, or DG16)
Chemical Recovery
1
Notes
linuron C Determination by DL3.
malathion C
methamidophos C
methidathion C
methiocarb C Determination by DL1.
methomyl C Determination by DL1.
methoxychlor, o, p'- C
methoxychlor, p, p'- C
metobromuron C Determination by DL3.
metoxuron C Determination by DL3.
mevinphos, (E)- C
mevinphos, (Z)- C
monocrotophos C
monolinuron C Determination by DL3.
monuron C Determination by DL3.
myclobutanil C
neburon C Determination by DL3.
omethoate C Wide bore or DEGS column required.
oryzalin C N or S detector required.
oxamyl C Determination by DL1.
parathion C
parathion oxygen analog C
parathion-methyl C
parathion-methyl oxygen analog C
penconazole C
pentachloroaniline C
permethrin, cis- C
permethrin, trans- C
phenylphenol, o- C
phorate sulfone C
phosalone C
phosmet C
pirimiphos-ethyl C
pirimiphos-methyl C
TABLE 302-f
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (10/1999)
Form FDA 2905a (6/92)TABLE 302-f–4
Table 302-f: Recovery Through 302 (E7 + C6 + DG1-DG3, DG6-DG7, DG10, DG13-
DG14, or DG16)
Chemical Recovery
1
Notes
procymidone C
propanil C
propiconazole C
prothiofos C
pyrazophos C
pyridaphenthion C
quintozene C
ronnel C (122%) n=1
simazine C
sulfotep C
TDE, p,p'- C
tetradifon C
thiabendazole C Determined by UV detector at 280 nm;
confirm, increase sensitivity with DL7.
THPI C
triadimefon C
triadimenol C
triazophos C
vinclozolin C
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–1
TABLE 303-a
Pesticide Analytical Manual Vol. I
Table 303-a: Recovery of Chemicals Through Method 303 (E1-E5 + C1 or
C2 + DG1-DG19)
(acetonitrile or water/acetonitrile extraction, partitioning into petroleum ether, Florisil
column cleanup, GLC determination with various columns and detectors)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
2
Recovery results refer to complete method; blank entry in this column indicates Florisil elution was tested but not complete
method. Separate results are listed for C1 and C2 only if recovery is affected by Florisil elution system.
3
Eluants(s) in which chemical is eluted from Florisil, according to directions in 303 C1, i.e., 6, 15, and 50% ethyl ether/
petroleum ether (EE/PE). Entries for chemicals not recovered indicate which eluants were used in tests.
4
Eluants(s) in which chemical is eluted from Florisil, according to directions in 303 C2, i.e., methylene chloride (CH
2
Cl
2
)
eluants #1, 2, and 3. Entries for chemicals not recovered indicate which eluants were used in tests.
5
“Florisil only” refers to tests in which elution patterns were tested by added reference standard solutions directly to Florisil
column.
6
Reference to petroleum ether (PE) forerun refers to Florisil elution performed as in 304 C3 or C4; not usually used in analysis
of nonfatty foods.
1,1'-(2,2-dichloroethylidene)= R
bis(2-methoxybenzene)
1,2,3,5-tetrachlorobenzene P (75%) 6
C1
1,2,3-trichlorobenzene C 6 1 Elutes in PE forerun.
1,2,4,5-tetrachloro-3-(methyl= C 6 1
thio)benzene
1,2,4-triazole NR 6-15-50 1-2-3
1-hydroxychlordene R 15
10,10-dihydromirex C 6
10-monohydromirex C 6
2,3,5,6-tetrachloroaniline R
2,3,5,6-tetrachloroanisidine C 6 2
2,3,5,6-tetrachloroanisole C 6 1
2,3,5,6-tetrachloronitroanisole C 6 1+2
2,3,5-trimethacarb S (18%) 50
NR 1-2-3
2,4,5-trichloro-alpha-methyl= R 15
benzenemethanol
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–2
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
2,4-dichloro-6-nitrobenzenamine R 15 2 Complete elution from Florisil
only in 15% EE/PE or CH
2
Cl
2
eluant #2.
2,6-dichlorobenzamide NR 6-15-50 1-2-3
2,8-dihydromirex C 6
2-chloroethyl caprate C 15 2
2-chloroethyl laurate C 15 2
2-chloroethyl linoleate V (36-114%) 15 2
2-chloroethyl myristate V (48-112%) 15 2
2-chloroethyl palmitate V (38-107%) 15 2
2-methoxy-3,5,6-trichloropyridine P(60-78%) 6+15
C 1+2
3,4,5-trimethacarb 50 Partial (20-35% ) elution from
Florisil only in 50% EE/PE.
NR 1-2-3
3,4-dichloroaniline S (8%) 15 35% elution from Florisil only
in 15% EE/PE.
3,4-dichlorophenylurea NR 6-15-50
3,5-dichloroaniline S (12-48%) 6+15 1+2 Partial (73%) elution from
Florisil only in 15% EE/PE.
3-(3,4-dichlorophenyl)-1- NR 6-15-50
methoxyurea
3-desmethyl sulfentrazone NR 6-15-50 1-2-3
3-hydroxymethyl-2,5-dimethyl= NR 6-15-50 1-2-3
phenyl methylcarbamate
3-ketocarbofuran NR 6 1 60% recovered from Florisil
only in 6% EE/PE or CH
2
Cl
2
#1; also elutes with PE.
3-methyl-4-nitrophenol NR 6-15-50 1-2-3
3-tert-butyl-5-chloro-6-hydroxy= NR 6-15-50 1-2-3
methyluracil
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–3
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
4-(dichloroacetyl)-1-oxa-4- P (50-62%) 50 3 Complete elution from Florisil
azapiro[4.5]decane only in 50% and in CH
2
Cl
2
eluant #3.
4-chloro-6-methoxyindole R 15
4-chlorobenzylmethyl sulfone NR 6-15-50 1-2-3
4-chlorobenzylmethyl sulfoxide NR 6-15-50 1-2-3
4-hydroxymethyl-3,5-dimethyl= NR 15-50 1-2-3 <20% elution from Florisil only
phenyl methylcarbamate in 15+50% EE/PE; <10% in
CH
2
Cl
2
eluants 1,2,3.
6-chloro-2,3-dihydro-3,3,7-methyl- NR 6-15-50 1-2-3
5H-oxazolo(3,2-a)pyrimidin-5-one
6-chloro-2,3-dihydro-7-hydroxy NR 6-15-50 1-2-3
methyl-3,3-methyl-5H-oxazolo=
(3,2-a)pyrimidin-5-one
6-chloronicotinic acid NR 6-15-50 1-2-3
8-monohydromirex C 6
acetochlor C (80-86%) 50
P (55-68%) 3 Complete elution from Florisil
only in CH
2
Cl
2
eluant #3.
acifluorfen NR 6-15-50 1-2-3
acrinathrin V(67-100%) 15
V(66-96%) 2
alachlor C 50
3 16% elution from Florisil only
in CH
2
Cl
2
eluant #3.
aldrin C 6 1
allethrin C 50 3
allidochlor NR 6-15 1-2-3
alpha-cypermethrin C 2
anilazine S (4-88%) 15+50 2+3
aramite P 15 Poor GLCsensitivity.
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–4
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
Aroclor 1016 C 6 1 Elutes in PE forerun.
Aroclor 1221 C 6 1 Elutes in PE forerun.
Aroclor 1242 C 6 1 Elutes in PE forerun.
Aroclor 1248 C 6 1 Elutes in PE forerun.
Aroclor 1254 C 6 1 Elutes in PE forerun.
Aroclor 1260 C 6 1 Elutes in PE forerun.
Aroclor 1262 C 6 1 Elutes in PE forerun.
Aroclor 1268 C 6 Elutes in PE forerun.
Aroclor 4465 C 6 1 Elutes in PE forerun.
atrazine S (25%) 50
NR 1-2-3
azinphos-ethyl P (50%) 50
3 49-79% elution from Florisil
only in CH
2
Cl
2
eluant #3.
azinphos-methyl NR 6-15-50 1-2-3
benfluralin C 6 2
benoxacor P 15+50 2+3 60-75% elution from Florisil
only in EE/PE; 40-80% in
CH
2
Cl
2
eluants.
bensulide P (70%) 50
3 14% elution from Florisil only
in CH
2
Cl
2
eluant #3.
benzoylprop-ethyl NR 6-15-50 1-2-3
BHC, alpha- C 6 1 Partially elutes in PE forerun.
BHC, beta- C 6 1
BHC, delta- C 6+15 1 EE/PE elution variable.
bifenox C 15+50 2+3
bifenthrin C 6+15 2
binapacryl P 15
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–5
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
bis(2-ethylhexyl) phthalate C 15+50 Poor EC detector sensitivity.
bis(trichloromethyl)disulfide R 6
bromacil NR 6-15-50 1-2-3
bromophos C 6
bromophos-ethyl C 6
bromopropylate C 15+50
NR 1-2-3
bromoxynil butyrate V (20-143%) 15+50 2
bromoxynil octanoate V (70-127%) 15+50
S (15-42%) 2
Bulan P (60%) 15
2 Complete elution from Florisil
only in CH
2
Cl
2
eluant #2.
butachlor C 50 Also complete (83%) recovery
from Florisil only in CH
2
Cl
2
eluant 3.
butralin C 6+15+50 Elution from Florisil variable.
butyl benzyl phthalate C 15+50
cadusafos NR 6-15-50 1-2-3
captafol P (75-80%) 50 3
captan P (75%) 50
P (50%) 3
captan epoxide NR 6-15
carbophenothion C 6 Elution from Florisil may be
variable.
2 <60% elution from Florisil only
in CH
2
Cl
2
eluant #2.
carbophenothion oxygen analog NR 6-15-50 1-2-3
carbophenothion sulfone C (80%) 6 1 Elutes in PE forerun.
carboxin NR 6-15-50
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–6
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
carboxin sulfoxide NR 6-15-50 1-2-3
CGA 118244 NR 6-15-50 1-2-3
CGA 120844 NR 6-15-50 1-2-3
CGA 14128 50 1-2-3 9-22% elution from Florisil
only in EE/PE; no elution in
CH
2
Cl
2
eluants.
CGA 171683 15+50 3 Complete elution from Florisil
only in 15+50% EE/PE, 70% in
CH
2
Cl
2
eluant #3.
CGA 205374 NR 6-15-50 1-2-3
CGA 37734 NR 6-15-50 1-2-3
CGA 91305 NR 6-15-50 1-2-3
CGA 94689A NR 6-15-50 1-2-3
CGA 94689B NR 6-15-50 1-2-3
chlorbenside S 6 1 Recovery 25-85% using EE/PE
eluants; may be better with
CH
2
Cl
2
.
chlorbromuron V (45-67%) 50 3 Complete elution from Florisil
only.
chlorbufam 15 2+3 Complete elution from Florisil
only in 15% EE/PE, 77% in
CH
2
Cl
2
eluants 2+3.
chlordane C 6 1
chlordane, cis- C 6 1 May elute in PE forerun.
chlordane, trans- C 6 1
chlordecone S (45%) 15+50 Elution from Florisil variable.
NR 1-2-3
chlordene C 6 1 Elutes in PE forerun.
chlordene epoxide C 15
chlorethoxyfos C 6 1
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–7
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
chlorfenvinphos, beta- S (0-49%) 50
NR 1-2-3
chlorimuron ethyl ester NR Variable (75-92%) elution
from Florisil only in 50% EE/
PE.
chlornitrofen C 6+15 2 Variable elution from Florisil
in EE/PE.
chlorobenzilate C 15+50
NR 3 Some variable elution from
Florisil only in eluant #3.
chloroneb C 6 2 82% elution from Florisil only
in CH
2
Cl
2
eluant #2.
chloropropylate C 15+50
3 Some variable elution from
Florisil only in CH
2
Cl
2
eluant
#3.
chlorothalonil NR 6-15-50
C 2+3
chlorothalonil trichloro impurity NR 6-15-50
R 2+3
chloroxuron NR 6-15-50 1-2-3
chlorpropham C 15 2
chlorpyrifos C 6 2
chlorpyrifos oxygen analog NR 6-15-50
chlorpyrifos-methyl C 6 2
chlorsulfuron NR 6-15-50
chlorthiophos C 6
2 11% elution from Florisil only
in CH
2
Cl
2
eluant #2.
chlorthiophos oxygen analog NR 6-15-50 1-2-3
chlorthiophos sulfone 50 55% elution from Florisil only
in 50% EE/PE.
C3
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–8
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
chlorthiophos sulfoxide NR 6-15-50 1-2-3
clofentezine S (15-24%) 15 2 Complete elution from Florisil
only; degrades on GLC in
extract.
clomazone 50 3 88% elution from Florisil only
in 50% EE/PE, 54-74% in
CH
2
Cl
2
eluant #3.
clopyralid methyl ester 50 17% elution from Florisil only
in 50% EE/PE.
Compound K C 1
coumaphos NR 6-15-50
3 Complete elution from Florisil
only in CH
2
Cl
2
eluant #3.
coumaphos oxygen analog NR 6-15-50 1-2-3
CP 51214 NR 6-15-50 1-2-3
crotoxyphos NR 6-15-50 1-2-3
crufomate NR 6-15-50
cyanazine NR 6-15-50
cycloate V (43-65%) 15+50
C3
cyfluthrin P (60%) 15
cymoxanil NR 6-15-50 1-2-3 Not eluted from Florisil.
cypermethrin C 15 2
cyproconazole NR 6-15-50 1-2-3
cyprodinil NR 6-15-50 1-2-3 Not eluted from Florisil.
dazomet NR 6-15-50 1-2-3
DCPA C 15 2
DDE, o,p'- C 6 1 Partially elutes in PE forerun.
DDE, p,p'- C 6 1 Elutes in PE forerun.
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–9
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
DDMS R 6
DDT, o,p'- C 6 1
DDT, p,p'- C 6 1
deltamethrin S (32-65%) 15 Very poor EC detector sensitiv-
ity.
C2
deltamethrin, trans- P (50-67%) 15
V (47-142%) 2
demeton-O NR 6-15
demeton-S NR 6-15-50
des N-isopropyl isofenphos S (30%) 50
desdiethyl simazine NR 6-15-50 1-2-3
desethyl simazine 50 43% elution from Florisil only
in 50% EE/PE.
NR 1-2-3
desisopropyl iprodione 50 1-2-3 17% eluted from Florisil only
with 50% EE/PE; not eluted
with CH
2
Cl
2
eluants.
desmethyl norflurazon NR 6-15-50 1-2-3
di-allate C 6
di-n-octyl phthalate C 15+50 Poor and variable EC detector
sensitivity.
dialifor C 15
2 Complete elution from Florisil
only in CH
2
Cl
2
eluant #2.
diazinon C 15 3
diazinon oxygen analog NR 6-15-50 1-2-3
dibutyl phthalate C 15+50
dichlobenil P 15 2
dichlofenthion C 6 2
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–10
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
dichlofluanid C 15+50
V (51-91%) 2+3 Elution from Florisil variable in
CH
2
Cl
2
eluants.
dichlone NR 6-15-50
S (30%) 2+3 Elution from Florisil variable.
dichlorobenzene, p- C 6 1
dichlorobenzophenone, o,p'- C 15 2
dichlorobenzophenone, p,p'- C 15 2
dichlorvos NR 6-15-50 1-2-3
diclobutrazol NR 6-15-50 1-2-3
diclofop-methyl C 15 2
dicloran S (35%) 15+50 2+3
dicofol, o,p'- V (50-100%) 6+15 2 Elution from Florisil may be
variable.
dicofol, p,p'- V (68-99%) 6+15 Elution from Florisil variable.
V (78-90%) 1+2
dicrotophos NR 6-15-50
dieldrin C 15 2
diethatyl-ethyl NR 6-15-50 1-2-3
diethyl phthalate P 15+50 Poor EC detector sensitivity.
diisobutyl phthalate P (75%) 15+50 About 80% elution from
Florisil only in 15+50% EE/PE.
diisohexyl phthalate C 15+50 Poor EC detector sensitivity.
diisooctyl phthalate C 15+50 Poor EC detector sensitivity.
Dilan P (65%) 15
dimethenamid NR 6-15-50 1-2-3
dimethipin NR 6-15-50 1-2-3
dimethoate NR 6-15-50 1-2-3
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–11
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
dimethomorph (prop) NR 6-15-50 1-2-3 Recovery tested using high
temperature column.
dimethyl phthalate P 6+15+50 Partial elution from Florisil
only in all EE/PE; poor EC
detector sensitivity.
dinocap P 15 75% elution from Florisil only
in 15% EE/PE.
2 Complete elution from Florisil
only in CH
2
Cl
2
eluant #2.
dioxabenzofos P (72%) 15
dioxathion NR 6-15-50
2 45% elution from Florisil only
in CH
2
Cl
2
eluant #2.
diphenamid NR 6-15
diphenylamine S (<20%) 6+15 <10% elution from Florisil only
in each 6 and 15% EE/PE.
disulfoton P (50-74%) 6 25-40% elution from Florisil
only in 6% EE/PE.
NR 1-2-3
disulfoton sulfone NR 6-15-50
diuron NR 6-15-50 1-2-3
endosulfan I C 15 2
endosulfan II C 15+50 2
endosulfan sulfate C 50 2
endrin C 15
V 2 60-90% elution from Florisil
only in CH
2
Cl
2
eluant #2.
endrin alcohol P (50%) 15+50
2+3 Partial (48%) elution from
Florisil only in CH
2
Cl
2
eluant
#2, 28% in #3.
endrin aldehyde P (50%) 15+50
endrin ketone C 50 2
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–12
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
EPN C 15
2 71% elution from Florisil only
in CH
2
Cl
2
eluant #2.
EPTC P (63%) 15
esfenvalerate C 15 2
ethalfluralin C 6 2 Elution from Florisil in CH
2
Cl
2
eluants may be variable.
ethametsulfuron methyl ester NR 6-15-50 1-2-3
ethephon 6+15+50 1+2+3 5-25% eluted from Florisil only
in each eluate.
ethiofencarb NR 6-15-50
ethion C 6
2 60-90% elution from Florisil
only in CH
2
Cl
2
eluant #2.
ethoprop P (55%) 50
NR 1-2-3
ethoxyquin NR 6-15-50
ethylenethiourea NR 6-15-50 1-2-3
etridiazole C 6 Other data show poor recovery
through C1.
2 Percent elution from Florisil
only varies in different reports.
etrimfos C 15 2+3
famphur NR 6-15-50
fenac NR 6-15-50
fenamiphos NR 6-15-50 1-2-3
fenamiphos sulfone NR 6-15-50 1-2-3
fenamiphos sulfoxide NR 6-15-50 1-2-3
fenarimol P (60%) 50 Quantitation may be influ-
enced by presence of sample
extract.
S (40%) 3
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–13
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
fenarimol metabolite B NR 6-15-50
fenarimol metabolite C 6 17% elution from Florisil only
in 6% EE/PE; no elution in 15
or 50% EE/PE.
fenbuconazole NR 6-15-50 1-2-3
fenitrothion C 15 2
fenoxaprop ethyl ester V (58-125%) 50 3 Partial (70%) elution from
Florisil only in either elution
system.
fenpropathrin V (43-71%) 15 Complete (111-116%) elution
from Florisil only in 15% EE/
PE.
P (55-65%) 2 Partial (56-62%) elution from
Florisil only in CH
2
Cl
2
eluant
#2.
fenpropimorph 50 Partial (49-63%) elution from
Florisil only in 50% EE/PE.
1-2-3 Not recovered from Florisil
only in CH
2
Cl
2
eluates.
fensulfothion NR 6-15-50 1-2-3
fensulfothion oxygen analog NR 6-15-50
fensulfothion sulfone NR 6-15-50
fenthion S (45%) 6+15
NR 1-2-3
fenthion oxygen analog NR 6-15-50 1-2-3
fenthion oxygen analog sulfoxide NR 6-15-50 1-2-3
fenthion sulfone NR 6-15-50 1-2-3
fenvalerate C 15 2
fipronil S (21-41%) 50 3
fluazifop butyl ester C 15 3 Poor EC detector sensitivity
with OV-225.
fluchloralin C 6 2
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–14
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
flucythrinate C 15
2+3 Elution from Florisil only 95%
in CH
2
Cl
2
eluant 2, 6% in
eluant 3.
fluridone NR 6-15-50
fluvalinate C 15
2 Complete elution in Florisil
only in CH
2
Cl
2
eluant 2.
folpet C 15+50
C (80%) 2+3 Complete elution from Florisil
only in CH
2
Cl
2
eluants #2 & 3.
fonofos C 6 2+3
fonofos oxygen analog NR 6-15-50 1-2-3
formothion NR 6-15-50 1-2-3
fosthiazate NR 6-15-50 1-2-3
furilazole S (28-50%) 50 3 Complete elution from Florisil
only in 50% EE/PE, CH
2
Cl
2
#3.
Gardona NR 6-15-50 1-2-3
GS-31144 NR 6-15-50 1-2-3
heptachlor C 6 1
heptachlor epoxide C 6 2
hexachlorobenzene C 6 1 Elutes in PE forerun.
hexachlorobutadiene V (62-88%) 6 Elutes in PE forerun.
P (78%) 1
hexachlorophene NR 6-15-50
hexachlorophene dimethyl ether NR 6-15
hexazinone NR 6-15-50 1-2-3
hexythiazox S (2-20%) 50 Florisil pattern and recoveries
vary; may elute in 15%, may be
complete.
C 2+3
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–15
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
hydroxy chloroneb NR 6-15
imazalil NR 6-15-50
imidacloprid NR 6-15-50 1-2-3
IN-A3928 NR 6-15-50 1-2-3
IN-B2838 NR 6-15-50 1-2-3
IN-T3936 NR 6-15-50 1-2-3
iprodione S (5-56%) 50
NR 1-2-3
iprodione metabolite isomer S (21-100%) 50
isazofos C 50 Recovery test performed on
corn grain and beef liver.
P 2+3
isofenphos C 15+50
isopropalin C 6
isoxaflutole (prop) V (60-120%) 50 Complete elution from Florisil
only in 50% EE/PE
NR 3 32-56% elution from Florisil
only in CH
2
Cl
2
eluant 3.
Korax NR 6-15
KWG 1323 NR 6-15-50 1-2-3
leptophos C 6 2
lindane C 6 1
linuron V (42-64%) 50
S (19-33%) 3
malathion C 15+50 3 Elution from Florisil variable in
EE/PE eluants.
malathion oxygen analog NR 6-15-50 1-2-3
MB45950 P (50-73%) 15+50 2+3
MB46136 S (28-55%) 50 2+3
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–16
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
mecarbam 50 Partial (43%) elution from
Florisil only in 50% EE/PE.
merphos C 6+15+50 Elution from Florisil variable.
3 Partial (48%) elution from
Florisil only in CH
2
Cl
2
eluant
#3.
metalaxyl NR 6-15-50 1-2-3
methabenzthiazuron NR 6-15-50 1-2-3
methidathion S (35%) 50
3 Complete elution from Florisil
only in CH
2
Cl
2
eluant #3.
methidathion oxygen analog NR 6-15-50 1-2-3
methidathion sulfone NR 6-15-50 1-2-3
methidathion sulfoxide NR 6-15-50 1-2-3
methiocarb sulfone NR 6-15-50 1-2-3
methomyl NR 6-15-50 1-2-3
methoxychlor olefin C 6 2
methoxychlor, o, p'- C 6
methoxychlor, p, p'- C 6 2
methyl 4-chloro-1H-indole-3-acetate R 50
NR 1-2-3 Not eluted from Florisil only in
CH
2
Cl
2
eluants.
metobromuron NR 6-15-50 1-2-3
metolachlor S (28-70%) 50
NR 1-2-3
metoxuron NR 6-15-50 1-2-3
metribuzin NR 50 1-2-3 Complete elution from Florisil
only in 50% EE/PE; may be S
recovery thru method.
metribuzin, deaminated diketo metabolite NR 6-15-50 1-2-3
metribuzin, deaminated metaboliteNR 6-15-50 1-2-3
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–17
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
metribuzin, diketo metabolite NR 6-15-50 1-2-3
mevinphos, (E)- NR 6-15-50
mevinphos, (Z)- NR 6-15-50
mirex C 6 1 Elutes in PE forerun.
monocrotophos NR 6-15-50 1-2-3
monuron NR 6-15-50 1-2-3
myclobutanil NR 6-15-50 1-2-3
myclobutanil alcohol metabolite NR 6-15-50 1-2-3
myclobutanil dihydroxy metabolite NR 6-15-50 1-2-3
N, N-diallyl dichloroacetamide S (41-51%) 15+50 2+3 Complete elution from Florisil
only in 15+50% EE/PE, CH
2
Cl
2
eluants 2+3.
N-(3,4-dichlorophenyl)-N'-methylurea NR 6-15-50
naled NR 6-15-50 1-2-3
neburon NR 6-15-50 1-2-3
nitralin P (60%) 50 50-80% elution from Florisil
only in 50% EE/PE.
3 75% elution from Florisil only
in CH
2
Cl
2
eluant #3.
nitrapyrin C 6 2 Complete elution from Florisil
only in 6% EE/PE or CH
2
Cl
2
eluant #2.
nitrofen C 15 2
nitrofluorfen C 15 2
nonachlor, cis- C 6 1
nonachlor, trans- C 6 1 Elutes in PE forerun.
norflurazon NR 6-15-50
NTN33823 NR 6-15-50 1-2-3
NTN35884 NR 6-15-50 1-2-3
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–18
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
nuarimol 50 35% elution from Florisil only
in 50% EE/PE.
NR 1-2-3
octachlor epoxide C 6 1
omethoate NR 6-15-50 1-2-3
oryzalin NR 6-15-50
ovex C 15 2
oxadiazon C 15
oxadixyl NR 6-15-50 1-2-3
oxamyl oxime metabolite NR 6-15-50 1-2-3
oxyfluorfen C 15 2 Poor N/P detector sensitivity.
parathion C 15 2
parathion oxygen analog NR 6-15-50 1-2-3
parathion-methyl C 15 2
parathion-methyl oxygen analog NR 6-15-50 1-2-3
PB-9 NR 6-15-50 1-2-3
pebulate P (70%) 15 68% elution from Florisil only
in 15% EE/PE; none eluted in
50%.
pendimethalin C 15 2
pentachloroaniline C 6 1
pentachlorobenzene C 6 1 Elutes in PE forerun.
pentachlorobenzonitrile C 15 2
pentachlorophenyl methyl ether C 6 1
pentachlorophenyl methyl sulfide C 6 1
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–19
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
permethrin, cis- V (60-115%) 6+15 Complete elution from Florisil
only in 6+15% EE/PE; high
temp column recommended.
C 2 High temperature column
recommended.
permethrin, trans- V (60-115%) 6+15 Complete elution from Florisil
only in 6+15% EE/PE; high
temp column recommended.
C 2 High temperature column
recommended.
Perthane C 6 1
Perthane olefin C 6 1
phenthoate C 15+50
phorate V (40-75%) 6 Elution from Florisil quite
variable.
C1
phorate oxygen analog NR 6-15-50 1-2-3
phorate oxygen analog sulfone NR 6-15-50 1-2-3
phorate oxygen analog sulfoxide NR 6-15-50 1-2-3
phorate sulfone NR 6-15-50
S (34-38%) 3 38% elution from Florisil only
in CH
2
Cl
2
eluant #3.
phorate sulfoxide NR 6-15-50 1-2-3
phosalone C 50 2+3
phosmet NR 6-15-50
3 Partial (60%) elution from
Florisil only in CH
2
Cl
2
eluant
#3.
phosmet oxygen analog NR 6-15-50
phosphamidon NR 6-15-50 1-2-3
photodieldrin C 15+50 2
pirimiphos-ethyl C 15+50 3
pirimiphos-methyl C 15 3
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–20
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
PPG-2597 NR 6-15-50 1-2-3
PPG-947 NR 6-15-50 1-2-3
procymidone C (84%) 15
profenofos P (65%) 50 Partial (56%) elution from
Florisil only in 50% EE/PE.
3 Partial (38%) elution from
Florisil only in CH
2
Cl
2
eluant
#3.
profluralin V (70-100%) 6 Complete elution from Florisil
only in 6% EE/PE.
Prolan S (40%) 15
2 Complete elution from Florisil
only in CH
2
Cl
2
eluant #2.
prometryn P (50%) 50 Variable (22-67%) elution
from Florisil only in 50% EE/
PE.
NR 1-2-3
pronamide P (63-71%) 15+50 39% elution from Florisil only
in 6% EE/PE, 24% in 50% EE/
PE.
propachlor NR 6-15-50 1-2-3 Trace amount may be eluted in
CH
2
Cl
2
eluant #3.
propanil NR 6-15
3 Partial (41%) elution from
Florisil only in CH
2
Cl
2
eluant
#3.
propargite C 15 2
propazine S (41%) 15+50 Complete but variable elution
from Florisil only in 15%+50%
EE/PE.
3 Also elution of trace amount
from Florisil only in CH
2
Cl
2
eluant #2.
propetamphos C (80%) 15+50
P (50%) 2+3
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–21
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
propham P (53%) 15 Addn 8-16% elution from
Florisil only in 50% EE/PE;
none in 50% thru method.
propiconazole NR 6-15-50 1-2-3
prosulfuron NR 6-15-50 1-2-3
prothiofos C 6
C 2 79% elution from Florisil only
in CH
2
Cl
2
eluant #2.
pyrazon NR 6-15-50 1-2-3
pyrazon metabolite B NR 6-15-50 1-2-3
pyrethrins C 50
pyrimethanil S (11-51%) 50 3 Complete elution from Florisil
only in 50% EE/PE or CH
2
Cl
2
eluant #3.
quinalphos C 15
quintozene C 6 1
RH-6467 NR 6-15-50 1-2-3
RH-9129 NR 6-15-50 1-2-3
RH-9130 NR 6-15-50 1-2-3
ronnel C 6 2
ronnel oxygen analog NR 6-15-50
RPA202248 NR 6-15-50 1-2-3
S-bioallethrin C 50
schradan NR 6-15-50
sethoxydim NR 6-15-50 3 CH
2
Cl
2
elution from Florisil
only tested with eluant #3 only,
not #1 or #2.
sethoxydim sulfoxide NR 6-15-50 3 CH
2
Cl
2
elution from Florisil
only tested with eluant #3 only,
not #1 or #2.
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–22
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
simazine NR 50 Complete elution from Florisil
only in 50% EE/PE.
NR 1-2-3
Strobane C 6 1
sulfallate C 6+15 2 Elution with EE/PE may be
variable.
sulfanilamide NR 6-15-50 1-2-3
sulfotep C 6+15 Wide bore column recom-
mended.
2 50% elution from Florisil only
in CH
2
Cl
2
eluant #2.
Sulphenone 50 3 Complete elution from Florisil
only in 20+25% EE/PE or in
CH
2
Cl
2
eluant #3.
TCMTB P (50-67%) 15 P (61-62%) elution from
Florisil only in 15% EE/PE; no
elution in 50% EE/PE.
TDE, o,p'- C 6 1
TDE, p,p'- C 6 1
TDE, p,p'-, olefin C 6 1 Partially elutes in PE forerun.
tebufenozide NR 6-15-50 1-2-3
tebupirimfos V (50-115%) 6+15 2+3 Elution from Florisil only also
variable.
tebupirimfos oxygen analog NR 6-15-50 1-2-3
tecnazene C 6 1
teflubenzuron NR 6-15-50 1-2-3
terbacil NR 6-15
2+3 30% elution from Florisil only
in CH
2
Cl
2
eluant #2, 13% in
eluant #3.
terbufos P (62%) 6
terbufos oxygen analog sulfone NR 6-15-50 1-2-3
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 303-a–23
TABLE 303-a
Pesticide Analytical Manual Vol. I
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
terbufos sulfone NR 6-15-50
C (77-86%) 2+3 Elution from Florisil only in
CH
2
Cl
2
eluant #2 (46%) and #3
(37%).
terbuthylazine P (57%) 15+50
tetradifon C 15 2
tetraiodoethylene P (65%) 6
tetramethrin NR 6-15-50 1-2-3 Trace amount may elute from
Florisil in CH
2
Cl
2
eluant #3.
tetrasul C 6 1
thiabendazole NR 6-15-50
thiobencarb 15 2+3 40% elution from Florisil only
in 15% EE/PE; 42% inCH
2
Cl
2
#2, 11% in CH
2
Cl
2
#3.
thiometon NR 6-15-50
thionazin P (59%) 15+50 Complete (80%) elution from
Florisil only in 15% and/or
50% EE/PE.
THPI NR 6-15-50
toxaphene C 6 1
tralkoxydim 50 20% elution from Florisil only
in 50% EE/PE.
NR 1-2-3
tralomethrin V (50-100%) 15 2
tri-allate C 6 2
triadimefon S (27-40%) 50 35% elution from Florisil only
in 50% EE/PE.
NR 1-2-3 No elution from Florisil in
CH
2
Cl
2
eluants.
triadimenol NR 6-15-50
triazamate NR 6-15-50 1-2-3
TABLE 303-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 303-a–24
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
Table 303-a: Recovery Through 303 (E1-E5 + C1 or C2 + DG1-DG19)
tribufos C 15+50
3 Partial, variable elution from
Florisil only in CH
2
Cl
2
eluant
#3.
tributyl phosphate R 50
trichlorfon NR 6-15-50 1-2-3
trichloronat C 6
tridiphane C 6
1+2 20% elution from Florisil only
in CH
2
Cl
2
eluant #1, 80% in #2.
trifluralin C 6 2
triflusulfuron methyl ester NR 6-15-50 1-2-3
tris(chloropropyl) phosphate NR 6-15-50 1-2-3
Tycor S (1-19%) 50 3 Complete elution from Florisil
only in 50% EE/PE, 50-60% in
CH
2
Cl
2
#3.
vernolate P (65%) 15
vinclozolin C 15 2
vinclozolin metabolite B P (55-66%) 6+15
V (60-105%) 2
vinclozolin metabolite E S (9-39%) 15+50
vinclozolin metabolite F NR 6-15-50 1-2-3
vinclozolin metabolite S P (55-70%) 15 2
WAK4103 NR 6-15-50 1-2-3
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–1
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery of Chemicals Through Method 304 (E1-E5 + C1-C4 + DG1-DG19)
(extraction of fat from fatty products, acetonitrile/petroleum ether partitioning,
Florisil column cleanup, GLC determination with various columns and detectors)
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
2
Recovery results refer to complete method; blank entry in this column indicates Florisil elution was tested but not complete
method. Separate results are listed for C1 and C2 only if recovery is affected by Florisil elution system used.
3
Eluants(s) in which chemical is eluted from Florisil, according to directions in 304 C1, i.e., 6, 15, and 50% ethyl ether/
petroleum ether (EE/PE). Entries for chemicals not recovered indicate which eluants were used in tests.
4
Eluants(s) in which chemical is eluted from Florisil, according to directions in 304 C2, i.e., methylene chloride (CH
2
C1
2
)
eluants #1, 2, and 3. Entries for chemicals not recovered indicate which eluants were used in tests.
5
“Florisil only” refers to tests in which elution patterns were tested by added reference standard solutions directly to Florisil
column.
6
Reference to petroleum ether (PE) forerun refers to Florisil elution performed as in 304 C3 or C4.
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
1,2,3-trichlorobenzene P (60%) 6 Elutes in PE forerun.
1 Complete elution from Florisil
only in CH
2
Cl
2
eluant #1; elutes
in PE forerun.
1,2,4-triazole NR 6-15-50 1-2-3
2,3,5-trimethacarb 50 50% elution from Florisil only
in 50% EE/PE eluant.
NR 1-2-3
2,4-dichloro-6-nitrobenzenamine 15 2
2,6-dichlorobenzamide NR 6-15-50 1-2-3
2-chloroethyl caprate C 15 2
2-chloroethyl laurate C 15 2
2-chloroethyl linoleate P (73-80%) 15 2
2-chloroethyl myristate V (42-80%) 15 2
2-chloroethyl palmitate P (50-59%) 15 2
2-methoxy-3,5,6-trichloropyridine C 6+15 1+2
3,4,5-trimethacarb 50 Partial (20-35% ) elution from
Florisil only in 50% EE/PE.
NR 1-2-3
3,4-dichlorophenylurea NR 6-15-50
3,5-dichloroaniline S (22-43%) 15 2
3-(3,4-dichlorophenyl)-1-methoxyurea NR 6-15-50
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–2
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
3-desmethyl sulfentrazone NR 6-15-50 1-2-3
3-hydroxymethyl-2,5-dimethyl= NR 6-15-50 1-2-3
phenyl methylcarbamate
3-ketocarbofuran NR 6 1 60% recovered from Florisil
only in 6% EE/PE or CH
2
Cl
2
#1; also elutes with PE.
3-methyl-4-nitrophenol NR 6-15-50 1-2-3
3-tert-butyl-5-chloro-6-hydroxy= NR 6-15-50 1-2-3
methyluracil
4-chlorobenzylmethyl sulfone NR 6-15-50 1-2-3
4-chlorobenzylmethyl sulfoxide NR 6-15-50 1-2-3
4-hydroxymethyl-3,5-dimethyl= NR 15-50 1-2-3 <20% elution from Florisil only
phenyl methylcarbamate in 15+50% EE/PE; <10% in
CH
2
Cl
2
eluants 1,2,3.
6-chloro-2,3-dihydro-3,3,7-methyl- NR 6-15-50 1-2-3
5H-oxazolo(3,2-a)pyrimidin-5-one
6-chloro-2,3-dihydro-7-hydroxy= NR 6-15-50 1-2-3
methyl-3,3-methyl-5H-oxazolo=
(3,2-a)pyrimidin-5-one
6-chloronicotinic acid NR 6-15-50 1-2-3
acetochlor P (52-70%) 15+50 2+3 Complete elution from Florisil
only in 50% EE/PE or CH
2
Cl
2
eluant #3.
acifluorfen NR 6-15-50 1-2-3
acrinathrin NR Complete elution from Florisil
only in 15% EE/PE.
V(27-80%) 2
alachlor C 50
S (23%) 3 16% elution from Florisil only
in CH
2
Cl
2
eluant #3.
aldrin C 6 1
allethrin C 50 Elution from Florisil in EE/PE
may be variable.
P (66-75%) 3
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–3
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
anilazine P 15+50 2+3
aramite NR
Aroclor 1016 C 6 1 Elutes in PE forerun.
Aroclor 1221 C 6 1 Elutes in PE forerun.
Aroclor 1242 C 6 1 Elutes in PE forerun.
Aroclor 1248 C 6 1 Elutes in PE forerun.
Aroclor 1254 C 6 1 Elutes in PE forerun.
Aroclor 1260 C 6 1 Elutes in PE forerun.
Aroclor 1262 C 6 1 Elutes in PE forerun.
Aroclor 4465 C 6 1 Elutes in PE forerun.
atrazine NR 1-2-3
azinphos-ethyl S (14%) 50
3 49-79% elution from Florisil
only in CH
2
Cl
2
eluant #3.
azinphos-methyl NR 6-15 1-2-3
benfluralin C 6 2
benoxacor C 15+50 2+3
bensulide C 50
3 14% elution from Florisil only
in CH
2
Cl
2
eluant #3.
benzoylprop-ethyl NR 6-15-50 1-2-3
BHC, alpha- C 6 1 Partially elutes in PE forerun.
BHC, beta- C 6 1
BHC, delta- C 6+15 1 EE/PE elution variable.
bifenox P (51-78%) 15+50 2+3 51-58% elution from Florisil
with EE/PE; 56-78% with
CH
2
Cl
2
.
bifenthrin 6+15 2
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–4
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
binapacryl P (65%) 15
bis(2-ethylhexyl) phthalate C 15+50 Poor EC detector sensitivity.
bromacil NR 6-15-50 1-2-3
bromophos C 6
bromophos-ethyl P (59-78%) 6
bromopropylate C 15+50
NR 1-2-3
Bulan P (75%) 15
2 Complete elution from Florisil
only in CH
2
Cl
2
eluant #2.
butyl benzyl phthalate P (70%) 15+50 Complete elution from Florisil
only in15+50% EE/PE.
cadusafos NR 6-15-50 1-2-3
captan C (80%) 50
carbophenothion P (60%) 6 Elution from Florisil may be
variable.
2 <60% elution from Florisil only
in CH
2
Cl
2
eluant #2.
carbophenothion oxygen analog NR 6-15-50 1-2-3
carbophenothion sulfone P (66%) 6 1 Elutes in PE forerun.
carboxin NR 6-15-50
carboxin sulfoxide NR 6-15-50 1-2-3
CGA 118244 NR 6-15-50 1-2-3
CGA 120844 NR 6-15-50 1-2-3
CGA 14128 50 1-2-3 9-22% elution from Florisil
only in EE/PE; not recovered
in CH
2
Cl
2
eluants.
CGA 171683 15+50 3 Complete elution from Florisil
only in 15+50% EE/PE, 70% in
CH
2
Cl
2
eluant #3.
CGA 205374 NR 6-15-50 1-2-3
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–5
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
CGA 37734 NR 6-15-50 1-2-3
CGA 91305 NR 6-15-50 1-2-3
CGA 94689A NR 6-15-50 1-2-3
CGA 94689B NR 6-15-50 1-2-3
chlorbenside P 6 1 Recovery 50% using EE/PE
eluants; may be better with
CH
2
Cl
2
.
chlorbromuron V (44-100%) 50 3 Complete elution from Florisil
only.
chlorbufam 15 2+3 Complete elution from Florisil
only in 15% EE/PE, 77% in
CH
2
Cl
2
eluants 2+3.
chlordane C 6 1
chlordane, cis- C 6 1 May elute in PE forerun.
chlordane, trans- C 6 1
chlordecone P 15+50 Elution from Florisil variable.
NR 1-2-3
chlordene C 6 1 Elutes in PE forerun.
chlorfenapyr (prop) S (30-50%) 50 2 Complete elution from Florisil
only in 50% EE/PE and
CH
2
Cl
2
eluant 2.
chlorfenvinphos, alpha- NR 6-15-50
chlornitrofen C 6+15 2 Variable elution from Florisil
in EE/PE.
chlorobenzilate P (75%) 15+50
NR 3 Some variable elution from
Florisil in CH
2
Cl
2
eluant #3.
chloroneb 2 82% elution from Florisil only
in CH
2
Cl
2
eluant #2.
chloropropylate C 15+50
3 Some variable elution from
Florisil in CH
2
Cl
2
eluant #3.
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–6
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
chlorothalonil NR 6-15-50
C (80-90%) 2+3
chlorothalonil trichloro impurity NR 6-15-50
chloroxuron NR 6-15-50 1-2-3
chlorpropham C 15 2
chlorpyrifos P (74-83%) 6 2
chlorsulfuron NR 6-15-50
chlorthiophos C 6
2 11% elution from Florisil only
in CH
2
Cl
2
eluant #2.
chlorthiophos oxygen analog NR 6-15-50 1-2-3
chlorthiophos sulfone 50 55% elution from Florisil only
in 50% EE/PE.
chlorthiophos sulfoxide NR 6-15-50 1-2-3
clomazone 50 3 88% elution from Florisil only
in 50% EE/PE, 54-74% in
CH
2
Cl
2
eluant #3.
clopyralid methyl ester 50 17% elution from Florisil only
in 50% EE/PE.
coumaphos NR 6-15-50
C (76-93%) 3 High temperature or short
column GLC needed.
coumaphos oxygen analog NR 6-15-50 1-2-3
CP 51214 NR 6-15-50 1-2-3
crotoxyphos NR 6-15-50 1-2-3
crufomate NR 6-15-50
cycloate S (39-61%) 15+50
S (24-37%) 3
cymoxanil NR 6-15-50 1-2-3 Not eluted from Florisil.
cypermethrin 15
C (81%) 2
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–7
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
cyproconazole NR 6-15-50 1-2-3
cyprodinil NR 6-15-50 1-2-3 Not eluted from Florisil.
DCPA C 15 2
DDE, o,p'- C 6 1 Partially elutes in PE forerun.
DDE, p,p'- C 6 1 Elutes in PE forerun.
DDT, o,p'- C 6 1
DDT, p,p'- C 6 1
deltamethrin P (77-80%) 15 2 Very poor EC detector sensitiv-
ity.
deltamethrin, trans- NR Partial (33%) elution from
Florisil only in 15% EE/PE,
complete in CH
2
Cl
2
eluant #2.
desdiethyl simazine NR 6-15-50 1-2-3
desethyl simazine 50 43% elution from Florisil only
in 50% EE/PE.
NR 1-2-3
desmethyl norflurazon NR 6-15-50 1-2-3
di-n-octyl phthalate C 15+50 Poor and variable EC detector
sensitivity.
dialifor P (50%) 15
2 Complete elution from Florisil
only in CH
2
Cl
2
eluant #2.
diazinon C 15 3
diazinon oxygen analog NR 6-15-50 1-2-3
dibutyl phthalate C 15+50
dichlobenil C (80%) 15 2
dichlofenthion V (69-89%) 6
2
dichlone NR 6-15-50
S (25%) 2+3 Elution from Florisil variable.
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–8
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
dichlorobenzene, p- C 6 1
dichlorobenzophenone, o,p'- C 15 2
dichlorobenzophenone, p,p'- C 15 2
dichlorvos NR 6-15-50 1-2-3
diclobutrazol NR 6-15-50 1-2-3
diclofop-methyl C 15 2
dicloran P (50%) 15+50 2+3
dicofol, o,p'- S (25-50%) 6+15 2 Elution from Florisil may be
variable.
dicofol, p,p'- P (61-85%) 6+15 Elution from Florisil variable.
S (36-58%) 1+2
dieldrin C 15 2
diethatyl-ethyl NR 6-15-50 1-2-3
diethyl phthalate P 15+50 Poor EC detector sensitivity.
diisobutyl phthalate 15+50 About 80% elution from
Florisil only in 15+50% EE/PE.
diisohexyl phthalate 15+50 Complete elution from Florisil
only in 15+50% EE/PE; poor
EC sensitivity.
diisooctyl phthalate C 15+50 Poor EC detector sensitivity.
Dilan P (65%) 15
dimethenamid NR 6-15-50 1-2-3
dimethipin NR 6-15-50 1-2-3
dimethoate NR 6-15-50 1-2-3
dimethomorph (prop) NR 6-15-50 1-2-3
dimethyl phthalate 6+15+50 Partial elution from Florisil
only in all EE/PE; poor EC
detector sensitivity.
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–9
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
dinitramine P (78-80%) 15 Some elution from Florisil in
6% EE/PE.
dinocap P (60%) 15 75% elution from Florisil only
in 15% EE/PE.
2 Elution from Florisil only,
complete in CH
2
Cl
2
eluant #2.
dioxathion 2 45% elution from Florisil only
in CH
2
Cl
2
eluant #2.
disulfoton 6 25-40% elution from Florisil
only in 6% EE/PE.
NR 1-2-3
diuron NR 6-15-50 1-2-3
endosulfan I C 15 2
endosulfan II C 15+50 2
endosulfan sulfate C 50 2
endrin C 15
V 2 60-90% elution from Florisil
only in CH
2
Cl
2
eluant #2.
endrin alcohol C 15+50
2+3 Partial (48%) elution from
Florisil only in CH
2
Cl
2
eluant
#2, 28% in #3.
endrin aldehyde C 15+50
endrin ketone C 50 2
EPN C 15
2 71% elution from Florisil only
in CH
2
Cl
2
eluant #2.
esfenvalerate C 15 2
ethalfluralin C 6 2 Elution from Florisil in CH
2
Cl
2
eluants may be variable.
ethametsulfuron methyl ester NR 6-15-50 1-2-3
ethephon 6+15+50 1+2+3 5-25% eluted from Florisil only
in each eluant.
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–10
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
ethiofencarb NR 6-15-50
ethion C 6
2 60-90% elution from Florisil
only in CH
2
Cl
2
eluant #2.
ethoprop S (45%) 50
NR 1-2-3
ethoxyquin NR 6-15-50
ethylenethiourea NR 6-15-50 1-2-3
etridiazole P (68-73%) 6 Other data shows poor recov-
ery through C1.
2 Percent elution from Florisil
only varies in different reports.
etrimfos C 15 2+3
fenac NR 6-15-50
fenamiphos NR 6-15-50 1-2-3
fenamiphos sulfone NR 6-15-50 1-2-3
fenamiphos sulfoxide NR 6-15-50 1-2-3
fenarimol C 50 Quantitation may be influ-
enced by presence of sample
extract.
V (72-110%) 3
fenarimol metabolite B NR 6-15-50
fenarimol metabolite C 6 17% elution from Florisil only
in 6% EE/PE; no elution in 15
or 50% EE/PE.
fenbuconazole NR 6-15-50 1-2-3
fenitrothion C 15 2
fenoxaprop ethyl ester V (65-110%) 50 3 Partial (70%) elution from
Florisil only in either elution
system.
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–11
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
fenpropathrin V (59-114%) 15 Elution from Florisil only,
complete (111-116%) in 15%
EE/PE.
V (58-91%) 2 Elution from Florisil only,
partial (56-62%) in CH
2
Cl
2
eluant #2.
fenpropimorph 50 Partial (49-63%) elution from
Florisil only in 50% EE/PE.
1-2-3 Not recovered from Florisil
only in CH
2
Cl
2
eluates.
fensulfothion NR 6-15-50 1-2-3
fenthion NR 6-15 1-2-3
fenthion oxygen analog NR 6-15-50 1-2-3
fenthion oxygen analog sulfoxide NR 6-15-50 1-2-3
fenthion sulfone NR 6-15-50 1-2-3
fenvalerate 15 Complete elution from Florisil
only in 15% EE/PE.
C (81%) 2
fipronil V (55-97%) 50 3
fluazifop butyl ester V (50-110%) 15 3 Poor EC detector sensitivity
with OV-225.
fluridone NR 6-15-50
folpet P (50%) 15+50
2+3 Complete elution from Florisil
only in CH
2
Cl
2
eluants #2 & 3.
fonofos C 6 2+3
fonofos oxygen analog NR 6-15-50 1-2-3
formothion NR 6-15-50 1-2-3
fosthiazate NR 6-15-50 1-2-3
Gardona NR 6-15-50 1-2-3
GS-31144 NR 6-15-50 1-2-3
heptachlor C 6 1
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–12
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
heptachlor epoxide C 6 2
hexachlorobenzene P (60%) 6 Loss in partitioning from PE to
acetonitrile/water; elutes in PE
forerun.
1 Complete elution from Florisil
only in CH
2
Cl
2
eluant #1; elutes
in PE forerun.
hexachlorobutadiene P (63%) 1 Elutes in PE forerun.
hexachlorophene NR 6-15-50
hexachlorophene dimethyl ether NR 6-15
hexazinone NR 6-15-50 1-2-3
hexythiazox NR 6-15-50 1-2-3 Complete elution from Florisil
only in 15+50% EE/PE, CH
2
Cl
2
eluants 2+3.
imazalil NR 6-15-50
imidacloprid NR 6-15-50 1-2-3
IN-A3928 NR 6-15-50 1-2-3
IN-B2838 NR 6-15-50 1-2-3
IN-T3936 NR 6-15-50 1-2-3
iprodione 50 Partial (4-19%) elution from
Florisil only in 50% EE/PE.
NR 1-2-3
isoxaflutole (prop) S (37-126%) 50 Complete elution from Florisil
only in 50% EE/PE.
NR 32-56% elution from Florisil
only in CH
2
Cl
2
eluant 3.
KWG 1323 NR 6-15-50 1-2-3
lactofen C 50 2+3
leptophos C 6 2
lindane C 6 1
linuron V (42-62%) 50
3
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–13
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
malathion C 15+50 3 Variable elution from Florisil
in EE/PE eluants.
malathion oxygen analog NR 6-15-50 1-2-3
MB45950 V (60-190%) 15+50 2+3
MB46136 V (54-140%) 50 2+3
mecarbam 50 Partial (43%) elution from
Florisil only in 50% EE/PE.
merphos C 6+15+50 Variable elution from Florisil
in EE/PE eluants.
3 Partial (48%) elution from
Florisil only in CH
2
Cl
2
eluant
#3.
metalaxyl NR 6-15-50 1-2-3
methabenzthiazuron NR 6-15-50 1-2-3
methidathion P (50%) 50
C (80%) 3
methidathion oxygen analog NR 6-15-50 1-2-3
methidathion sulfone NR 6-15-50 1-2-3
methidathion sulfoxide NR 6-15-50 1-2-3
methiocarb sulfone NR 6-15-50 1-2-3
methomyl NR 6-15-50 1-2-3
methoxychlor olefin C 6 2
methoxychlor, p, p'- C 6 2
methyl 4-chloro-1H-indole- NR 1-2-3 Not eluted from Florisil only in
3-acetate CH
2
Cl
2
eluants.
metobromuron NR 6-15-50 1-2-3
metolachlor NR 1-2-3
metoxuron NR 6-15-50 1-2-3
metribuzin NR 50 1-2-3 Complete elution from Florisil
only in 50% EE/PE; may be S
recovery thru method.
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–14
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
metribuzin, deaminated diketo NR 6-15-50 1-2-3
metabolite
metribuzin, deaminated NR 6-15-50 1-2-3
metabolite
metribuzin, diketo metabolite NR 6-15-50 1-2-3
mevinphos, (E)- NR 6-15-50
mirex P (75%) 6 1 Loss in partitioning from PE to
acetonitrile/water; elutes in PE
forerun.
monocrotophos NR 6-15-50 1-2-3
monuron NR 6-15-50 1-2-3
myclobutanil NR 6-15-50 1-2-3
myclobutanil alcohol metabolite NR 6-15-50 1-2-3
myclobutanil dihydroxy NR 6-15-50 1-2-3
metabolite
N, N-diallyl dichloroacetamide S (32-47%) 15+50 2+3 Complete elution from Florisil
only in 15+50% EE/PE, CH
2
Cl
2
eluants 2+3.
N-(3,4-dichlorophenyl)-N'- NR 6-15-50
methylurea
naled NR 6-15-50 1-2-3
neburon NR 6-15-50 1-2-3
nitralin P (70%) 50 50-80% elution from Florisil
only in 50% EE/PE.
3 75% elution from Florisil only
in CH
2
Cl
2
eluant #3.
nitrapyrin V (32-111%) 6 2 Complete elution from Florisil
only in 6% EE/PE or CH
2
Cl
2
eluant #2.
nitrofen C 15 2
nitrofluorfen C 15 2
nonachlor, cis- C 6 1
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–15
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
nonachlor, trans- C 6 1 Elutes in PE forerun.
norflurazon NR 6-15-50
NTN33823 NR 6-15-50 1-2-3
NTN35884 NR 6-15-50 1-2-3
nuarimol C 50 35% elution from Florisil only
in 50% EE/PE.
NR 1-2-3
octachlor epoxide C 6 1
omethoate NR 6-15-50 1-2-3
oryzalin NR 6-15-50
ovex C 15 2
oxadiazon P (75%) 15
oxadixyl NR 6-15-50 1-2-3
oxamyl oxime metabolite NR 6-15-50 1-2-3
oxyfluorfen C 15 2
parathion C 15 2
parathion oxygen analog NR 6-15-50 1-2-3
parathion-methyl C 15 2
parathion-methyl oxygen analog NR 6-15-50 1-2-3
PB-9 NR 6-15-50 1-2-3
pendimethalin P (33-56%) 15
P (66-82%) 2
pentachloroaniline C 6 1
pentachlorobenzene C 6 1 Elutes in PE forerun.
pentachlorobenzonitrile P (60%) 15
2 Complete elution from Florisil
only in CH
2
Cl
2
eluant #2.
pentachlorophenyl methyl ether C 6 1
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–16
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
pentachlorophenyl methyl sulfide C 6 1
permethrin, cis- 6+15 Complete elution from Florisil
only in 6+15% EE/PE; high
temp column recommended.
C (82%) 2 High temperature column
recommended.
permethrin, trans- 6+15 Complete elution from Florisil
only in 6+15% EE/PE; high
temp col recommended.
C (82%) 2 High temperature column
recommended.
Perthane C 6 1
Perthane olefin C 6 1
phorate V (80%) 6 Elution from Florisil quite
variable, may be 0%.
C1
phorate oxygen analog NR 6-15-50 1-2-3
phorate oxygen analog sulfone NR 6-15-50 1-2-3
phorate oxygen analog sulfoxide NR 6-15-50 1-2-3
phorate sulfone NR 6-15-50
S (12-20%) 3 38% elution from Florisil only
in eluant 3.
phorate sulfoxide NR 6-15-50 1-2-3
phosalone C 50 2+3
phosmet 3 Partial (60%) elution from
Florisil only in CH
2
Cl
2
eluant
#3.
phosmet oxygen analog NR 6-15-50
phosphamidon NR 6-15-50 1-2-3
photodieldrin C 15+50 2
pirimiphos-ethyl C 15+50 3
pirimiphos-methyl C 15 3
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–17
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
PPG-1576 P 50 2+3 72-85% elution from Florisil
only in EE/PE; 54-75% in
CH
2
Cl
2
eluants.
PPG-2597 NR 6-15-50 1-2-3
PPG-947 NR 6-15-50 1-2-3
procymidone P (76%) 15
profenofos P (50%) 50 Partial (56%) elution from
Florisil only in 50% EE/PE.
3 Partial (38%) elution from
Florisil only in CH
2
Cl
2
eluant
#3.
Prolan S (25%) 15
2 Complete elution from Florisil
only in CH
2
Cl
2
eluant #2.
prometryn P (70%) 50 Variable (22-67%) elution
from Florisil only in 50% EE/
PE.
NR 1-2-3
propachlor NR 6-15-50 1-2-3 Trace amount may be eluted in
CH
2
Cl
2
eluant #3.
propanil NR 6-15
3 Partial (41%) elution from
Florisil only in CH
2
Cl
2
eluant
#3.
propargite 15 2 Complete elution from Florisil
only in 15% EE/PE or CH
2
Cl
2
eluant #2.
propazine NR 15+50 Complete but variable elution
from Florisil only in 15%+50%
EE/PE.
3 Also elution of trace amount
from Florisil only in CH
2
Cl
2
eluant #2.
propham P (80%) 15 Addition 8-16% elution from
Florisil only in 50% EE/PE;
none in 50% thru method.
propiconazole NR 6-15-50 1-2-3
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–18
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
prosulfuron NR 6-15-50 1-2-3
prothiofos C 6
2 79% elution from Florisil only
in CH
2
Cl
2
eluant #2.
pyrazon NR 6-15-50 1-2-3
pyrazon metabolite B NR 6-15-50 1-2-3
pyrethrins C 50
pyrimethanil S (0-40%) 50 3 Complete elution from Florisil
only in 50% EE/PE or CH
2
Cl
2
eluant 3.
P (75-82% 50 3
quintozene C 6 1
RH-6467 NR 6-15-50 1-2-3
RH-9129 NR 6-15-50 1-2-3
RH-9130 NR 6-15-50 1-2-3
ronnel C 6 2
RPA202248 NR 6-15-50 1-2-3
sethoxydim NR 6-15-50 3 CH
2
Cl
2
elution from Florisil
tested with eluant #3 only, not
#1 or #2.
sethoxydim sulfoxide NR 6-15-50 3 CH
2
Cl
2
elution from Florisil
tested with eluant #3 only, not
#1 or #2.
simazine 50 Complete elution from Florisil
only in 50%EE/PE.
NR 1-2-3
Strobane C 6 1
sulfallate C 6+15 2 Elution with EE/PE may be
variable.
sulfanilamide NR 6-15-50 1-2-3
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–19
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
sulfotep P (65-70%) 6+15 2 65% elution from Florisil with
EE/PE; 70% with CH
2
Cl
2
; need
wide bore column.
Sulphenone 50 3 Complete elution from Florisil
only in 20+25% EE/PE or in
CH
2
Cl
2
eluant #3.
TCMTB P (61-62%) 15 P (61-62%) elution from
Florisil only in 15% EE/PE; no
elution in 50% EE/PE.
TDE, o,p'- C 6 1
TDE, p,p'- C 6 1
TDE, p,p'-, olefin C 6 1 Partially elutes in PE forerun.
tebufenozide NR 6-15-50 1-2-3
tebupirimfos V (57-171%) 6+15 2+3 Elution from Florisil only also
variable.
tebupirimfos oxygen analog NR 6-15-50 1-2-3
tecnazene C 6 1
teflubenzuron NR 6-15-50 1-2-3
terbacil NR 6-15
2+3 30% elution from Florisil only
in CH
2
Cl
2
eluant #2, 13% in
eluant #3.
terbufos S (16%) 6
terbufos oxygen analog NR 6-15-50 1-2-3
terbufos oxygen analog sulfone NR 6-15-50 1-2-3 No elution from Florisil in
either elution system.
terbufos sulfone NR 6-15-50
C 2+3 Elution from Florisil only in
CH
2
Cl
2
eluant #2 (46%) and #3
(37%).
tetradifon C 15 2
tetraiodoethylene P (65%) 6
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–20
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
tetramethrin NR 6-15-50 1-2-3 May be elution of trace amount
from Florisil in CH
2
Cl
2
eluant
#3.
tetrasul C 6 1
thiobencarb V (<50-86%) 15 40% elution from Florisil only
in 15% EE/PE.
2+3 42% elution from Florisil only
in CH
2
Cl
2
#2, 11% in CH
2
Cl
2
#3.
thiometon NR 6-15-50
thionazin NR 15+50 Complete (80%) elution from
Florisil only in 15% and/or
50% EE/PE.
THPI NR 6-15-50
toxaphene C 6 1
tralkoxydim 50 20% elution from Florisil only
in 50% EE/PE.
NR 1-2-3
tralomethrin S (0-50%) 15 2
tri-allate C 6 2
triadimefon S (13-62%) 50 35% elution from Florisil only
in 50% EE/PE.
NR 1-2-3 No elution from Florisil in
CH
2
Cl
2
eluants.
triadimenol NR 6-15-50
triazamate NR 6-15-50 1-2-3
tribufos P (60%) 15+50
3 Partial, variable elution from
Florisil only in eluant #3.
trichlorfon NR 6-15-50 1-2-3
trichloronat 6 Complete elution from Florisil
only in 6% EE/PE.
tridiphane 6 1+2 20% elution from Florisil only
in CH
2
Cl
2
eluant #1, 80% in #2.
Transmittal No.2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-a–21
TABLE 304-a
Pesticide Analytical Manual Vol. I
Table 304-a: Recovery Through 304 (E1-E5 + C1-C4 + DG1-DG19)
Chemical Recovery
1,2
Eluant, C1
3
Eluant, C2
4
Notes
5,6
trifluralin C 6 2
triflusulfuron methyl ester NR 6-15-50 1-2-3
tris(chloropropyl) phosphate NR 6-15-50 1-2-3
Tycor S (12-162%) 50 3 Complete elution from Florisil
only in 50% EE/PE, 50-60% in
CH
2
Cl
2
#3.
vinclozolin C 15 2
vinclozolin metabolite B C 6+15 2 Recovery in 6% EE/PE; other
studies showed split into 15%.
vinclozolin metabolite E NR 6-15-50
vinclozolin metabolite F NR 6-15-50 1-2-3
vinclozolin metabolite S V (47-81%) 15
C2
WAK4103 NR 6-15-50 1-2-3
TABLE 304-a
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-a–22
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-b–1
TABLE 304-b
Pesticide Analytical Manual Vol. I
Table 304-b: Recovery of Chemicals Through Method 304 (E1-E5 + C6 + DG1-DG19)
(extraction of fat from fatty products, cleanup with gel permeation and Florisil column
chromatography, GLC determination with various columns and detectors)
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
2
Eluants(s) in which chemical is eluted from Florisil, according to directions in 304 C6, i.e., methylene chloride (CH
2
Cl
2
)
eluants #1, 2, and 3. Entries for chemicals not recovered indicate which eluants were used in tests.
Chemical Recovery
1
Eluant
2
Notes
1,2,3,5-tetrachlorobenzene V (41-138%) 1 mean recovery 85.9%, n=15
1,2,4,5-tetrachloro-3-(methylthio)= C 1 mean recovery 86%, n=11
benzene
2,3,5,6-tetrachloroanisidine V (47-108%) 2 mean recovery 82.8%, n=10
2,3,5,6-tetrachloroanisole C 1 mean recovery 89.6%, n=10
2,3,5,6-tetrachloronitroanisole V (47-135%) 1+2 mean recovery 76.4%, n=10
2-chloroethyl linoleate V (0-102%) 2 mean recovery 66.86%, n=7
2-chloroethyl palmitate V (0-105%) 2 mean recovery 68.0%, n=7
alachlor S (0-121%) 3 mean recovery 47.6%, n=13
aldrin C 1 mean recovery 85.2%, n=21
alpha-cypermethrin C 2 mean recovery 89.3%, n=12
anilazine S (4-87%) 2+3 mean recovery 32%, n=11
bromopropylate NR 1-2-3
captan S (0-88%) 3 mean recovery 33.4%, n=13
carbophenothion NR 1-2-3 mean recovery 3%, n=12
chlorfenvinphos, beta- NR 1-2-3 mean recovery 2%, n=13
chlornitrofen C 2 69-114% recovered, TDS
chlorobenzilate NR 1-2-3
chlorothalonil S (0-86%) 2+3 mean recovery 37%, n=26
chlorpropham C 2 mean recovery 82%, n=14
chlorpyrifos C 2 mean recovery 83%, n=39
chlorpyrifos-methyl C 2 mean recovery 83.4%, n=14
cyfluthrin P 2 mean recovery 77.8%, n=14
DDE, p,p'- P 1 recovery 75.3%, n=1
DDT, o,p'- C 1 mean recovery 88.2%, n=13
diazinon C 3 69-114% recovery, TDS
dichlofenthion C 2 mean recovery 87.5%, n=12
diclofop-methyl C 2 mean recovery 88.1%, n=25
dicloran V (49-111%) 2+3 mean recovery 76.8%, n=12
dieldrin C 2 mean recovery 87.7%, n=130
TABLE 304-b
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-b–2
Table 304-b: Recovery Through 304 (E1-E5 + C6 + DG1-DG19)
Chemical Recovery
1
Eluant
2
Notes
endosulfan I C 2 mean recovery 89.0%, n=12
endosulfan sulfate C 2 mean recovery 88.9%, n=27
endrin C 2 recovery 82.7%, n=1
esfenvalerate C 2 mean recovery 88.8%, n=12
ethion oxygen analog NR 1-2-3
fenarimol S (0-58%) 3 mean recovery 13.5%, n=13
fenthion NR 1-2-3 mean recovery 0.5%, n=13
fenvalerate V (69-130%) 2 mean recovery 91%, n=14
fluchloralin C 2 mean recovery 81%, n=11
haloxyfop methyl ester C 2+3 mean recovery 91.3%, n=4 , 80%
eluant 2 remainder in 3.
heptachlor C 1 recovery 96.7%, n=1
heptachlor epoxide C 2 mean recovery 84.4%, n=28
iprodione S (0-52%) 3 mean recovery 16.2%, n=16 ; trace
amount eluated by elauant 3.
iprodione metabolite isomer V (12-120%) 3 mean recovery 73.2%, n=28 .
lindane C 1 mean recovery 87.2%, n=40
linuron V (43-114%) 3 mean recovery 73.5%, n=24
mecarbam V (13-92%) 3 mean recovery 71.6%, n=15 .
methidathion C 3
methoxychlor, o, p'- C 2 83-124% recoveries, TDS
methoxychlor, p, p'- C 2 mean recovery 88.2%, n=12
metolachlor NR 1-2-3
mirex C 1 mean recovery 89.4%, n=17
nonachlor, cis- C 1
nuarimol NR 1-2-3
octachlor epoxide C 1 mean recovery 90.5%, n=32
parathion C 2 mean recovery 81.7%, n=82
pentachlorophenyl methyl sulfide C 1 mean recovery 84.7%, n=17
Perthane C 1 mean recovery 87.5%, n=15
phenthoate P mean recovery 76.0%, n=12; eluant
data to be tested.
phosalone S (17-78%) 2+3 mean recovery 39.8%, n=16
phosmet S 3 37%, 67% recoveries, TDS
pirimiphos-ethyl V (29-109%) 3 mean recovery 67.2%, n=13
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-b–3
TABLE 304-b
Pesticide Analytical Manual Vol. I
Table 304-b: Recovery Through 304 (E1-E5 + C6 + DG1-DG19)
Chemical Recovery
1
Eluant
2
Notes
propargite P 2 mean recovery 79.5%, n=12
prothiofos P 2 mean recovery 71.9%, n=13
pyrazophos C recovery 107%, n=1 ; eluant data to be
tested.
sulprofos NR 1-2-3
TDE, o,p'- C 1 mean recovery 95.9%, n=15
TDE, p,p'- C 1 mean recovery102.4%, n=14
TDE, p,p'-, olefin C 1 mean recovery 86.0%, n=13
tecnazene C 1 58-108% recoveries, TDS
tetradifon C 2
tridiphane C 1+2 mean recovery 84.5%, n=13
vinclozolin C 2 mean recovery 83.3%, n=12
TABLE 304-b
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-b–4
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92) TABLE 304-c–1
TABLE 304-c
Pesticide Analytical Manual Vol. I
Table 304-c: Recovery of Chemicals Through Method 304 (E2 + C7 + DG1-DG19)
(extraction of fat from fatty products, Florisil column cleanup, GLC determination with
various columns and detectors)
Chemical Recovery
1, 2
Eluant
3
Notes
1
Codes: C: complete (>80%); P: partial (50-80%); S: small (<50%); V: variable (approximate percentage when known);
R: recovered but no quantitative information available; NR: not recovered.
2
Recovery results do not include the optional alkaline hydrolysis step.
3
Eluants(s) in which chemical is eluted from Florisil, according to directions in 304 C7, i.e., 6 and 15% ethyl ether/petroleum
ether (EE/PE). Entries for chemicals not recovered indicate which eluants were used in tests.
aldrin C 6 mean recovery 94.8%, n=19
Aroclor 1254 C 6 mean recovery 80.0%, n=18
chlordane C 6 recovery 83.0%, n=1
chlordane, cis- C 6 recovery 93.0%, n=1
chlordane, trans- C 6 recovery 124%, n=1
chlorpyrifos C 6 mean recovery 88.0%, n=12
DDE, p,p'- C 6 recovery 107%, n=1
DDT, p,p'- C 6 mean recovery 87.2%, n=8
diazinon C 15 mean recovery 107%, n=1
dieldrin C 15 mean recovery 97.1%, n=31
heptachlor epoxide C 6
nonachlor, cis- C 6 recovery 103%, n=1
nonachlor, trans- S(0.8-32%) 6 mean recovery 16.4%, n=2
octachlor epoxide C 6 recovery 83.0%, n=1
pentachlorobenzene C 6 recovery 100%, n=1
TDE, p,p'- V (59-93%) 6 mean recovery 75.5%, n=4
TABLE 304-c
Pesticide Analytical Manual Vol. I
Transmittal No. 2000-1 (11/1999)
Form FDA 2905a (6/92)TABLE 304-c–2
| 课件名称： | 美国FDA农残分析手册（第一巻英文） |
| 课件分类： | 化学 |
| 课件类型： | 电子图书 |
| 文件大小： | 3.02MB |
| 下载次数： | 0 |
| 评论次数： | 0 |
| 用户评分： | 0 |
- 1. 美国FDA农残分析手册（第一巻英文）：appndx1
- 2. 美国FDA农残分析手册（第一巻英文）：appndx2
- 3. 美国FDA农残分析手册（第一巻英文）：ch3tabs
- 4. 美国FDA农残分析手册（第一巻英文）：ch4tabs
- 5. 美国FDA农残分析手册（第一巻英文）：chapter1
- 6. 美国FDA农残分析手册（第一巻英文）：chapter2
- 7. 美国FDA农残分析手册（第一巻英文）：chapter3
- 8. 美国FDA农残分析手册（第一巻英文）：chapter4
- 9. 美国FDA农残分析手册（第一巻英文）：chapter5
- 10. 美国FDA农残分析手册（第一巻英文）：chapter6
- 11. 美国FDA农残分析手册（第一巻英文）：indices
- 12. 美国FDA农残分析手册（第一巻英文）：Introduction