Radiation Chemistry Ionizing radiation produces abundant secondary electrons that rapidly slow down (thermalize) to energies below 7.4 eV, the threshold to produce electronic transitions in liquid water. For both the primary charged particle and the secondary electrons, this slowing down process is accomplished by transfer of energy to the medium in a sequence of discrete events. Stopping power (-dE/dx) treats the slowing down process as a continuous function, sometimes referred to as the continuous slowing down approximation. Depending on the amount of energy transferred to the electron, the molecule can undergo: ? Ionization (threshold in water ~ 13 eV) ? excitation (threshold in water ~ 7.4 eV) ? thermal transfer Image removed. The example shown is for 20 keV electrons. [Thermal transfers (vibration, rotation, translation) are largely inconsequential. A dose of 10 4 Gy would be necessary before the thermal effects (a few degrees centigrade) became large enough to affect cellular biochemistry.] 1 Timescale of radiation chemistry Image removed. 2 Initial Physical Events The initial event is the transfer of ~ 7 - 100 eV, an amount of energy sufficient to cause (multiple) ionizations or excitations in water molecules. Transfer of energy to the medium in biological systems usually involves ionization of a water molecule, but can also involve the cellular macromolecules (e.g., DNA) directly. Through ionizations and excitations the passage of a charged particle through biological medium creates three species in the local vicinity of the particle track: Direct ionization of water produces a radical ion and a free subexcitation electron (E < 7.4 eV). H 2 O H?→? rad 2 O· + + eˉ Energy transfer can produce a water molecule in an excited state. H 2 O H?→? rad 2 O* The time scale for the creation of these species is on the order of 10 -16 seconds. Prechemical Reactions The three initial species begin to diffuse and react with each other or other molecules in the medium. Some of these reactions produce radicals. null Radical refers to an atom or molecule that contains an unpaired electron. null Radicals are highly reactive. null Radicals can be neutral or charged. 3 The electron is captured by water through dipolar interactions, becoming solvated, and referred to as an aqueous electron or a solvated electron: eˉ + H 2 O → surrounded by a “cage” of water; ? aq e eˉ + H + → H· or it can react with H + to form a radical. ? The radical ion of water can dissociate to produce a hydroxyl radical and a hydrogen ion. H 2 O· + → H + + HO· ? The excited water molecule can dissipate excess energy by bond breakage to produce hydroxyl and hydrogen radicals. H 2 O* → HO· + H· It takes ~ 5 eV to break the O-H bond. Image removed. Example of the dissociation of excited water to form hydroxyl and hydrogen radicals (from Tubiana, 1990). 4 The actual concentrations of the radicals are very small, especially when compared to the concentrations of ions present from the dissociation of water. Thus, the three initial species: H 2 O * , H 2 O· + and eˉ, react further to produce chemically reactive species: HO·, H·, and , ? aq e Water radiolysis produces highly reactive HO· and H· radicals. These radicals are much more reactive than HOˉ or H + from ionic dissociation. HO· is a powerful oxidizing agent, very reactive chemically. Oxidation: the loss of electrons. The electrons are transferred to the oxidizing agent, which becomes reduced. Reduction: the addition of electrons. May involve the addition of electron only, or the addition of hydrogen together with an electron. Image removed. 5 Chemical Stage After ~10 -12 sec, the chemically reactive species are still located in the vicinity of the original H 2 O * , H 2 O· + and eˉ species that caused their creation. Three of the new species created are radicals: HO·, H·, . ? aq e These species now begin to migrate randomly about their initial positions. As this diffusion proceeds, individual pairs may come close enough together to react with each other. A variety of reactions are possible in the track of the charged particle. HO· + HO· → H 2 O 2 HO· + → OH ? aq e ? HO· + H· → H 2 O H + + → H· ? aq e ? aq e + + 2 H ? aq e 2 O → H 2 + 2 OH ? ? aq e + H· + H 2 O → H 2 + OH ? H· + H· → H 2 Most of these reactions remove chemically reactive species from the system. With time (by ~ 10 -6 sec) all of the reactive species have diffused sufficiently far that further reactions are unlikely. The chemical development of the track is over by 10 -6 sec. 6 Radical Diffusion Diffusion Constants D and Reaction Radii R for Reactive Species (after Turner J. E. Atoms, Radiation, and Radiation Protection, 2 nd ed. New York: Wiley- Interscience, 1995. Table 13.2) Species D (10 -5 cm 2 s -1 ) R (? ) OH 2 2.4 e – aq 5 2.1 H 3 O + 8 0.30 H 8 0.42 If the measured diffusion constant for a given species is D, then, on average, it will move a small distance, λ, in a time, τ, such that D= τ λ 6 2 The reaction radius, R, is a measure of the reactivity of the individual species. If a reactive species diffuses closer to a “target” than the reactive radius. It will react. Simulations of Charged-Particle Tracks The diffusion formula and the recombination reactions described above allow Monte Carlo simulation of the charged-particle tracks. Monte Carlo codes are used to model: ? passage of the charged particle, ? generation of secondary electrons, ? generation of chemically reactive species, ? diffusion of the reactive species through a series of “random walk” jumps, ? recombination events in any pairs that come closer than the reactive radius. 7 Examples of Track Simulations Image removed. Fig. 13.1 in Turner J. E. Atoms, Radiation, and Radiation Protection, 2 nd ed. New York: Wiley-Interscience, 1995. When the electron stops, in the upper left region of the figure, the track density is higher. This illustrates the high-LET nature of the electron track end. Image removed. Fig. 13.2 in [Turner]. High-LET particle (proton) produces a straight track, in contrast to the torturous tracks of electrons. High LET particles have a dense formation of reactants along the particle track. In the simulation, clusters and spurs are also generated along the track. 8 Examples of Simulated Particle Tracks Image removed. Fig. 13.3 in [Turner]. Expanded view of the high-LET proton track. Expanded scales show a close-up view of track development as a function of time. Image removed. Fig. 7.1 in [Turner]. Protons and alpha particles of the same velocity. The alpha particle has 4 times higher LET. 9 Radiochemical Yield: G values In simulations, the numbers of the various chemical species can be tabulated as a function of time. G value: the number of a particular species produced per 100 ev of energy loss by the charged particle and its secondaries when it stops in water. ? Some species will decrease with time, others will increase with time. ? By about 10 -6 sec, all chemical development of the track is over. G values will not change much after that. G values (number per 100 eV) for various species at 10 -7 s for protons of several energies and for alpha particles of the same velocities (Table 13.4 in [Turner].) ? The alpha particle has 4 times higher LET. ? LET decreases at higher energies, the initial density of reactants is lower, more survive without recombining (lines 1-4), less produced (lines 4+5). ? The alpha track is denser, the G values are lower, because more recombine. null Electrons, protons and alpha particles all produce the same species in track regions (at 10 -15 sec), H 2 O * , H 2 O· + and eˉ. null The chemical (and biological) differences later are due to the different spatial patterns of energy deposition along the track, ie., track density. 10 Effect of LET on G values Image removed. 11 Direct Action, Indirect Action and the Oxygen Effect ? So far, the radiation chemistry of water has been considered. ? It is possible that energy can be deposited directly in the biological molecule of interest (e.g., DNA). ? The result would still be ionization and/or excitation leading to radical formation in the biological molecule. ? Biological radicals can undergo reactions similar to those described for water. Image removed. Fig. 1.8 in Hall, Eric J. Radiobiology for the Radiologist, 5 th ed. Philadephia PA: Lippincott Williams & Wilkins, 2000. Direct action: energy deposition directly in the biological molecule (e.g., the DNA as shown here). The dose response relationship should be linear. Indirect action: Relies on the ionization of the solvent molecules as intermediaries. Indirect action is diffusion limited, the dose response relationship can be complex. Water radiolysis produces many reactive species. Experiments with scavengers specific for individual reactive species have shown that it is primarily the hydroxyl radical (HO·) that is responsible for radiation damage to DNA. 12 For high-LET radiation, direct action is the predominant mechanism of DNA damage. Image removed. Fig. 1.11 in [Hall]. Radiation damage in DNA can be repaired by several processes. ? Recombination: reaction of nearby radical with the DNA radical to regenerate the original DNA. Timescale is < 10 -11 sec. ? Restitution: chemical restoration of the DNA; no enzyme involved. There are a number of intracellular reducing agents that can react with radicals, the most important of which is glutathione (GSH). The sulfhydryl group on GSH can donate H· to the DNA, producing the restored DNA and a more stable (and less reactive) sulfur radical. The time scale is < ~ 10 -3 sec. DNA· + GSH → DNA + GS· ? Repair: there are many cellular enzymes that recognize and repair DNA damage. Time scale is minutes to hours. “Fixation” of damage by oxygen ? O 2 is a powerful oxidizing agent. O 2 has two unpaired electrons: a stable biradical. ? O 2 reacts readily with organic radicals: DNA· + O 2 → DNA-O-O· (DNA hydroperoxy radical) DNA-O-O· H· → DNA-O-OH (DNA hydroperoxide) ? If oxygen reacts with the DNA radical before it is repaired, the damage becomes harder, if not impossible, to repair. Cannot be repaired by chemical restitution. Image removed. Fig. 6.3 in [Hall]. 13 Scavengers Chemicals that can react with reactive species, like HO·, can block the indirect effects of radiation. Image removed. Scavengers ? Provide a way to estimate relative contributions of direct and indirect effects. ? Scavengers for specific reactive species can help define the radiation chemistry. “Clustered Damage” Image removed. Clustered damage is more difficult, if not impossible, for cells to repair and is thought to be the main reason that high-LET radiation is more damaging to cells than low-LET radiation. 14