?
—— ——

-
1-3
C
1 953 4 5
1957
40
—
—
1 9 96 1 27
3 1/4
—— G -
—— 50ml
—— 1ml
E.Coli
—— 1ml
-
C1


5-7 7
10%
pH
GMP
G-
G +
Pen chrysogenum
30000-60000?/mL
S
N
O
R C O N H
CH 3
CH 3
C O O H
C
C H 3
_ C O O H
NH 2
C H 3 CH __
O
NH _ C H -
25 C,6-
7d
25 C,6-
7d
25 C,40-
45d
1:2VVM
25 C,40-
45d
1:1.5VVM
22-26 C,6-7d
1:1-0.8VVM
15
C
G
0.1%
I
II
III
IV
V
VI
I-IV
III
IV-V
VI
25-26 C 23 C
pH 6.4-6.6
1 0.8VVM
30%
pH CO2 O2
0.01-0.05%
0.05-
0.08%
pH2
1/3
1.3-
1.9%NaHCO3 pH6.8-7.1
pH2
1 5 0-300g/10
0.5mol/LNaOH pH
6.4-6.8 3-4 16-
26 C 5mmHgG
2-4%
Streptomyces Rimosus
37oC
4-5
32?0.5oC 31?0.5oC
30-32h 24-26h
32oC -31?0.5oC - 32oC
160h
= 1 1
121 1 6 0
2h
0.3mL
6-
12 h
0-4
250 ml
4% 2% 2%
400-500L
4m3
15%
α-
KH2PO4 N aCl CaCO3
CoCl2
0-80 h 6.5-8%
80-120 h 5-6.5 %
120-140 h 4-5 %
140 h 4%
3% 24 h
pH pH=6.0-6.3
8 h
350-400
1
2
3 Fe3+
+
pH1.7
30(26000 r/ml)
7500-8000 r/ml
+
pH4.1-4.7
50
80-90oC
122#