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Scientia Agricultura Sinica doi,10.3864/j.issn.0578-1752.2009.01.040
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Depletion of Enrofloxacin and Its Metabolite in the Milk
of Lactating Cows
LIAO Jie-dan
1,2
,LI Shou-jun
1
,DING Huan-zhong
1
,LIU Wen-zi
1
,YU Jing-xian
1
,ZHANG Jia-hui
1
,LIU Ya-hong
1
(
1
College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642;
2
College of Life Science,Foshan
University,Foshan 528000,Guangdong)
Abstract, }Objective ~ Depletion of enrofloxacin and its metabolite in the milk were studied in mastitis cows after a single
intramammary dose of 500 mg per quarter, }Method ~ Concentration of enrofloxacin and its metabolite ciprofloxacin were
determined by high-performance liquid chromatography with fluorescence detector and the concentration-time data were analyzed by
statistical moment principle, }Result ~ Enrofloxacin and its metabolite ciprofloxacin were all at the peak after 2 hours in the
administered quarter,The concentrations were (13.16±3.10) μg·ml
-1
and (2.79±0.94) μg·ml
-1
,respectively,Enrofloxacin and its
metabolite ciprofloxacin can not be detected after 12 hours and 24 hours,In the healthy quarter enrofloxacin was at the peak after 1
hour and the concentration was (0.19±0.02) μg·ml
-1
,Enrofloxacin can not be detected after 6 hours,Ciprofloxacin was at the peak
after 4 hours and the concentration was (0.51±0.07) μg·ml
-1
,Ciprofloxacin can not be detected after 24 hours,In the administered
quarter,ciprofloxacin showed longer t
1/2β
which is (1.67±0.20) h in milk and maintained longer period than enrofloxacin,Under the
experiment condition,it is suggested that the clinical withdrawal time should not be less than three days, }Conclusion ~ The results
showed that enrofloxacin in mastitis cows is of rapid absorption,metabolism and elimination,Enrofloxacin and its metabolite
ciprofloxacin are not residuary in milk,
Key words,enrofloxacin; metabolite; depletion; cows; mastitis
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Fig,Enrofloxacin and ciprofloxacin concentration-time data
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Table 1 Concentration of enrofloxacin and ciprofloxacin in milk after intramammary administration enrofloxacin at the dose of 500
mg per quarter in mastitis lactating cows
6
v ì? Enrofloxacinìd ì? Ciprofloxacin
HW
Time (h)
ó0 u *
Administered quarters
dó0 u
Non-administered quarters
ó0 u *
Administered quarters
dó0 u
Non-administered quarters
0.5 - 0.13±0.02 - 0.14±0.04
1 - 0.19±0.02 - 0.21±0.06
2 13.16±3.1 0.09±0.01 2.79±0.94 0.39±0.06
4 1.25±0.31 0.04±0.01 1.25±0.39 0.51±0.07
6 0.11±0.03 ND 0.39±0.13 0.27±0.03
8 0.06±0.03 ND 0.12±0.02 0.15±0.02
10 0.02±0.01 ND 0.07±0.02 0.08±0.01
12 ND ND 0.05±0.01 0.05±0.01
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Table 2 Pharmacokinetic parameters of enrofloxacin and ciprofloxacin after intramammary administration enrofloxacin at the dose
of 500 mg per quarter in mastitis lactating cows
? u Quarters X ±S.E 0t
Drugs
?
Parameters
?ê
Units
ó0 u Administered quarters dó0 u Non-administered quarters
AUC mg·h
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·L
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MRT h 1.31±0.23 /
β h
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6
v ì?
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t
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h 0.81±0.14* /
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·L
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[9] ; [,fk ,
/
d ?\ ￥ù?éZ,!2D
,2007,(1),
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:,6
v ì? r B#
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/
?W￥1?0?D,?S
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[12]
??,
>?(,?}0 ?D,?,?SxD SvD#?D
SvD ó?
,1994,107-122,
1
ù
±?? 6
v ì?# }?át
/
d ??￥h"
p 317
Song Z Y,Liu G T,Current Pharmacology,Beijing Chinese Union
Medical University and Beijing Medical University Unified
Publishiing Company,1994,107-122,(in Chinese)
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[26] ~
bá,?Yé,ìd ì?
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ío?￥ ?\
/ ?0?D
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i,?S
u0D,2000,20(3),271-274,
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3 ?I
kd
j< SD 2009,42(1):312-317
Scientia Agricultura Sinica doi,10.3864/j.issn.0578-1752.2009.01.040
là °
ù 2008-01-16 ¤
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ù 2008-12-08
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2005J1-C0111
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Vù?Z_1
uD0 ?DDy ?D b Tel 0757-85505213 E-mail liaojiedan@163.com bY?
T?
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1966
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uD0 ?DDy ?D b Tel 020-85287189 E-mail gale@scau.edu.cn
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ir?Kú′
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d ?\
Depletion of Enrofloxacin and Its Metabolite in the Milk
of Lactating Cows
LIAO Jie-dan
1,2
,LI Shou-jun
1
,DING Huan-zhong
1
,LIU Wen-zi
1
,YU Jing-xian
1
,ZHANG Jia-hui
1
,LIU Ya-hong
1
(
1
College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642;
2
College of Life Science,Foshan
University,Foshan 528000,Guangdong)
Abstract, }Objective ~ Depletion of enrofloxacin and its metabolite in the milk were studied in mastitis cows after a single
intramammary dose of 500 mg per quarter, }Method ~ Concentration of enrofloxacin and its metabolite ciprofloxacin were
determined by high-performance liquid chromatography with fluorescence detector and the concentration-time data were analyzed by
statistical moment principle, }Result ~ Enrofloxacin and its metabolite ciprofloxacin were all at the peak after 2 hours in the
administered quarter,The concentrations were (13.16±3.10) μg·ml
-1
and (2.79±0.94) μg·ml
-1
,respectively,Enrofloxacin and its
metabolite ciprofloxacin can not be detected after 12 hours and 24 hours,In the healthy quarter enrofloxacin was at the peak after 1
hour and the concentration was (0.19±0.02) μg·ml
-1
,Enrofloxacin can not be detected after 6 hours,Ciprofloxacin was at the peak
after 4 hours and the concentration was (0.51±0.07) μg·ml
-1
,Ciprofloxacin can not be detected after 24 hours,In the administered
quarter,ciprofloxacin showed longer t
1/2β
which is (1.67±0.20) h in milk and maintained longer period than enrofloxacin,Under the
experiment condition,it is suggested that the clinical withdrawal time should not be less than three days, }Conclusion ~ The results
showed that enrofloxacin in mastitis cows is of rapid absorption,metabolism and elimination,Enrofloxacin and its metabolite
ciprofloxacin are not residuary in milk,
Key words,enrofloxacin; metabolite; depletion; cows; mastitis
?y
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Fig,Enrofloxacin and ciprofloxacin concentration-time data
V ?\
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μg·ml
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X ±S.E,n=6
Table 1 Concentration of enrofloxacin and ciprofloxacin in milk after intramammary administration enrofloxacin at the dose of 500
mg per quarter in mastitis lactating cows
6
v ì? Enrofloxacinìd ì? Ciprofloxacin
HW
Time (h)
ó0 u *
Administered quarters
dó0 u
Non-administered quarters
ó0 u *
Administered quarters
dó0 u
Non-administered quarters
0.5 - 0.13±0.02 - 0.14±0.04
1 - 0.19±0.02 - 0.21±0.06
2 13.16±3.1 0.09±0.01 2.79±0.94 0.39±0.06
4 1.25±0.31 0.04±0.01 1.25±0.39 0.51±0.07
6 0.11±0.03 ND 0.39±0.13 0.27±0.03
8 0.06±0.03 ND 0.12±0.02 0.15±0.02
10 0.02±0.01 ND 0.07±0.02 0.08±0.01
12 ND ND 0.05±0.01 0.05±0.01
24 ND ND ND ND
,-” V
U??""
,ND” ? V?¤,-”,Not collect sample,“ND”,Not detectable,*,n=5
1
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1
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Pìd ì?h"?
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h?16
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￥2 b
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d?4
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9
ó0 u6
v ì?￥ t
1/2β
aAUC′sY 0.81 h
196.18 mg·h
-1
·L
-1
ìd ì?￥ t
1/2β
MRT
ü (ì
=
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MRT (?6
v ì?é b¨d9 e ?9
6
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nV 2 b
V ?\
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d?4
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v ì?a6
v ì?ìd ì?￥0?D?
n=6
Table 2 Pharmacokinetic parameters of enrofloxacin and ciprofloxacin after intramammary administration enrofloxacin at the dose
of 500 mg per quarter in mastitis lactating cows
? u Quarters X ±S.E 0t
Drugs
?
Parameters
?ê
Units
ó0 u Administered quarters dó0 u Non-administered quarters
AUC mg·h
-1
·L
-1
196.18±64.15 /
MRT h 1.31±0.23 /
β h
-1
1.00±0.14
6
v ì?
Enrofloxacin
t
1/2β
h 0.81±0.14* /
AUC mg·h
-1
·L
-1
16.19±7.65 3.02±0.52
MRT h 4.01±0.40 5.92±0.38
β h
-1
0.42±0.04 0.28±0.05
ìd ì?
Ciprofloxacin
t
1/2β
h 1.67±0.20 3.00±0.24
* n=3 / V
U?
9
/,Not calculation
)
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3 ?I
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