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j< SD 2009,42(1):312-317
Scientia Agricultura Sinica doi,10.3864/j.issn.0578-1752.2009.01.040
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Depletion of Enrofloxacin and Its Metabolite in the Milk
of Lactating Cows
LIAO Jie-dan
1,2
,LI Shou-jun
1
,DING Huan-zhong
1
,LIU Wen-zi
1
,YU Jing-xian
1
,ZHANG Jia-hui
1
,LIU Ya-hong
1
(
1
College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642;
2
College of Life Science,Foshan
University,Foshan 528000,Guangdong)
Abstract,}Objective~ Depletion of enrofloxacin and its metabolite in the milk were studied in mastitis cows after a single
intramammary dose of 500 mg per quarter,}Method~ Concentration of enrofloxacin and its metabolite ciprofloxacin were
determined by high-performance liquid chromatography with fluorescence detector and the concentration-time data were analyzed by
statistical moment principle,}Result~ Enrofloxacin and its metabolite ciprofloxacin were all at the peak after 2 hours in the
administered quarter,The concentrations were (13.16±3.10) μg·ml
-1
and (2.79±0.94) μg·ml
-1
,respectively,Enrofloxacin and its
metabolite ciprofloxacin can not be detected after 12 hours and 24 hours,In the healthy quarter enrofloxacin was at the peak after 1
hour and the concentration was (0.19±0.02) μg·ml
-1
,Enrofloxacin can not be detected after 6 hours,Ciprofloxacin was at the peak
after 4 hours and the concentration was (0.51±0.07) μg·ml
-1
,Ciprofloxacin can not be detected after 24 hours,In the administered
quarter,ciprofloxacin showed longer t
1/2β
which is (1.67±0.20) h in milk and maintained longer period than enrofloxacin,Under the
experiment condition,it is suggested that the clinical withdrawal time should not be less than three days,}Conclusion~ The results
showed that enrofloxacin in mastitis cows is of rapid absorption,metabolism and elimination,Enrofloxacin and its metabolite
ciprofloxacin are not residuary in milk,
Key words,enrofloxacin; metabolite; depletion; cows; mastitis
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Table 1 Concentration of enrofloxacin and ciprofloxacin in milk after intramammary administration enrofloxacin at the dose of 500
mg per quarter in mastitis lactating cows
6
v ì? Enrofloxacinìd ì? Ciprofloxacin
HW
Time (h)
ó0 u *
Administered quarters
dó0 u
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ó0 u *
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dó0 u
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1 - 0.19±0.02 - 0.21±0.06
2 13.16±3.1 0.09±0.01 2.79±0.94 0.39±0.06
4 1.25±0.31 0.04±0.01 1.25±0.39 0.51±0.07
6 0.11±0.03 ND 0.39±0.13 0.27±0.03
8 0.06±0.03 ND 0.12±0.02 0.15±0.02
10 0.02±0.01 ND 0.07±0.02 0.08±0.01
12 ND ND 0.05±0.01 0.05±0.01
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Table 2 Pharmacokinetic parameters of enrofloxacin and ciprofloxacin after intramammary administration enrofloxacin at the dose
of 500 mg per quarter in mastitis lactating cows
? u Quarters X ±S.E 0t
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