http://spxy.zjgsu.edu.cn 《酶制剂与酶工程（英）》教学大纲 (Enzyme Engineering) 一、基本信息 课程代码：1040822 学 分：2 总 课 时：32 课程性质：专业选修课 适用专业：生物工程本科 先修课程：微生物学，生物化学 二、本课程的教学目的和任务 The Goal of this course: By studying this course, students can understanding kinetics, preparation and purification, and immobilization of enzymes. Also, knowing of applications and reactors of enzyme is another important task of students. Finally, students should master something in enzyme catalysis in organic media and some common, useful industrial enzymes. 三、教学方法与手段 本课程采用多媒体教学手段进行课堂讲授, 辅以读写议等方法。 四、教学内容与要求 Chapter 1: Fundamentals of enzyme kinetics Students should master: the mechanism of enzyme action and the simple kinetics of enzyme action. Students should understand enzyme inhibition and effect of pH and temperature on enzyme catalysis. Key point of this chapter: the simple kinetics of enzyme action. Difficult point in this chapter: Determination of V and K max m Chapter 2 Enzyme preparation and purification Students should master: the main useful methods of preparation and purification of enzymes. Students should understand screening methods of enzymes and measurements of enzyme activity. Key point of this chapter: the main useful methods of preparation and purification of enzymes, such as cell breakage, use of lytic methods, filtration, and chromatography. Difficult point in this chapter: affinity chromatography Chapter 3 Enzyme Immobilization Students should master: the methods of enzyme immobilization and kinetics of immobilized enzyme. Students should understand common usefulness of immobilized enzymes. http://spxy.zjgsu.edu.cn Key point of this chapter: the methods of enzyme immobilization and kinetics of immobilized enzyme. Difficult point in this chapter: kinetics of immobilized enzymes. Chapter 4: The large-scale use of enzymes in solution Students should master: the most useful applications of enzymes in food industry, leather and wool industry, and detergents. Students should understand the most useful applications of enzymes in food industry, leather and wool industry, and detergents. Key point of this chapter: the useful application of enzymes in food and dairy industry. Difficult point in this chapter: medical applications of enzymes. Chapter 5 Enzyme reactors Students should master: the main reactors used in production of enzymes, such as membrane reactor, packed bed reactor, and fluidized bed reactors. Students should understand continuous flow reactors and continuous flow stirred tank reactors. Key point of this chapter: the main reactors used in production of enzymes, such as membrane reactor, packed bed reactor, and fluidized bed reactors. Difficult point in this chapter: continuous flow stirred tank reactors. Chapter 6 Some important industrial enzymes Students should master: the most important industrial enzymes such as amylase and protease. Students should understand the production of lipase and phytase. Key point of this chapter: the production and main applications of amylase and protease. Difficult point in this chapter: molecular biology of xylanase and phytase. Chapter 7 Enzyme catalysis in organic media Students should master: the mechanism of enzyme reaction in biphasic liquid systems and the simple kinetics of enzyme catalysis in aqueous-organic systems. Students should understand the advantages of enzyme catalysis in organic media and some practical uses of enzyme catalysis in organic media.. Key point of this chapter: the mechanism of enzyme reaction in biphasic liquid systems and the simple kinetics of enzyme catalysis in aqueous-organic systems. Difficult point in this chapter: Determination of V and K max m kinetics of enzyme catalysis in organic media. Chapter 8: Future prospects for enzyme technology Students should master: the future prospects of enzyme engineering. Students should understand where enzyme engineering will go? Key point of this chapter: the future prospects of enzyme engineering. 五、教学时数分配表 Chapters Titles Reference time units 1 Fundamentals of enzyme and enzyme kinetics 4 2 Enzyme preparation and purification 4 http://spxy.zjgsu.edu.cn 3 Enzyme Immobilization 3 4 The large-scale use of enzymes in solution 3 5 Enzyme reactors 2 6 Some important industrial enzymes 6 7 Enzyme catalysis in organic media 4 8 Future prospects for enzyme technology 2 Reading, writing and discussing 4 total 32 六、考核方式与成绩评定标准 考核方式：采用闭卷考与写论文相结合 成绩评定标准：闭卷考占 70%，论文成绩占 30%。 七、教材和主要参考书 教材：章亭洲自编，Enzyme Engineering，first edition, 2006.1 参考书目： [1] 袁勤生, 赵健主编, 《酶与酶工程》，华东理工大学出版社，第 1 版，2005 年 8 月 [2] Colin J. Suckling. Enzyme Chemistry: Impact and applications. 2 nd edition. By Chapman and Hall Publishers. 1990. [3] Alejandro G. Marangoni. Enzyme kinetics, first edition, by Wiley-interscienc, 2003 执笔人：章亭洲