http://spxy.zjgsu.edu.cn 《细胞工程概论（英）》教学大纲 （ Introduction of cell engineering） 一、 基本信息 课程代码：1010722 学 分 ：2 总 课 时 ：32 课程性质：选修课 适用专业：生物工程 先修课程：生物化学 二、本课程教学目的和任务 教学目的：在学习动、植物等真核细胞基本结构、组成等基本知识的基础上，重点学习 高等动、植物细胞的体外培养技术，包括培养条件、培养方式等。掌握动、植物细胞原代和 继代培养的基本特点，细胞培养前的准备，有限细胞系与无限细胞系、细胞的保存、复苏、 运输，培养细胞的观察，培养细胞的生物学检查，细胞工程技术的应用等。 教学任务：通过本课程学习，使学生对动、植物等真核细胞基本结构、组成等基本知识 有一定的认识。重点掌握动、植物细胞培养前的准备、细胞原代和继代培养的基本特点、培 养细胞的生物学检查及细胞工程技术在生产实际中的应用等。 三、教学方法与手段 以课堂讲授为主。 四、教学内容及要求 Part I Fundamental Techniques in Cell Culture 1.0 The ECACC and its Collections 2.0 Design and Equipment for the Cell Culture Laboratory Including Laboratory Design, Microbiological Safety Cabinets, Centrifuges, Incubators, Work Surfaces and Flooring, Plasticware and Consumables, Care and Maintenance of Laboratory Areas. 3.0 Safety Aspects of Cell Culture Including Risk Assessment, Disinfection, Waste Disposal. 4.0 Sourcing of Cell Lines Introducing the basic concepts of cell lines 5.0 Main Types of Cell Culture Introducing the main types of cell culture, including the Primary Cultures, the continuous cultures, and Culture Morphology. 6.0 The Cell Environment http://spxy.zjgsu.edu.cn 6.1 Basic Constituents of media 6.2 Inorganic Salts 6.3 Buffering Systems 6.4 Carbohydrates 6.5 Vitamins 6.6 Proteins and Peptides 6.7 Fatty Acids and Lipids 6.8 Trace Elements 6.9 Serum 6.10 Guidelines for serum use 6.11 Origin of Serum 7.0 Cryopreservation and Storage of Cell Lines 7.1 Cryopreservation of Cell Lines 7.2 Ultra-low Temperature Storage of Cell Lines 7.3 Safety Considerations 8.0 Good Cell Banking Practices 8.1 Risk of microbial contamination 8.2 Loss of characteristics of interest (i.e. surface antigen or monoclonal antibody expression) 8.3 Genetic drift particularly in cells known to have an unstable karyotype (i.e. CHO Prod. No. 85050302-1v1, BHK 21 Prod. No. 85011433-1v1) 8.4 Loss of cell line due to exceeding finite life-span e.g. human diploid cells such as MRC-5 (Prod. No. 84101801-1v1) 8.5 Risk of cross contamination with other cell lines 8.6 Increased consumables and staff costs 9.0 Quality control considerations 9.1 Reagents and Materials 9.2 Provenance and Integrity of Cell Lines 9.3 Avoidance of Microbial Contamination 9.4 Environmental Monitoring 9.5 What to do in the event of contamination 10.0` Authentication of cell line 10.1 Authentication Techniques 10.2 Iso-Enzyme Analysis 10.3 DNA Fingerprinting 10.4 Multi Locus DNA Fingerprinting 10.5 Multiplex - PCR (STR) DNA profiling Part II Discussion — Advance in Cell signaling http://spxy.zjgsu.edu.cn Part III: Manipulate the medical plant cell as a small natural product factory : part of the work done in our laboratory 五、教学时数分配表 章 内 容 参考时数 1 Part I: Fundamental Techniques in Cell Culture 22 2 Part II: Discussion — Advance in Cell signaling 5 3 Part III: Manipulate the medical plant cell as a small natural product factory : part of the work done in our laboratory 5 合 计 32 六、考核方式及成绩评定标准 考核方式：闭卷 成绩评定标准：平时成绩占 30%，期末考试成绩占 70%。 七、教材及主要参考书 教材： Fundamental Techniques in Cell Culture, Sigma Nitric oxide mediates the fungal elicitor-induced hypericin production of Hypericum perforatum cell suspension cultures through a jasmonic acid-dependent signal pathway, Xu maojun et al., Plant Physiology, 2005 139:991 参考书目： [1] Molecular cell biology [2] Biotechnology and Bioengineering [3] Applied Microbiology and Biotechnology 八、其它说明 如有新版教材，及时更新使用。 执笔人: 徐茂军