http://spxy.zjgsu.edu.cn 《生物分离工程(英)》教学大纲 （ Bioseparation Engineering Science） 一、基本信息 课程代码：1005312 学 分 ：2 总 课 时 ：32 课程性质：专业选修课 适用专业：生物工程专业 先修课程：化工原理、生化工程、生物工程专业英语、生物化学、酶学、微生物学等 二、本课程教学目的和任务 本课程为生物工程专业本科段专业课程，主要指生物体内物质及其代谢产物，特别是生 物大分子的分离、检测、制备与改造技术。通过课程的学习，使学生了解生物体系的基本特 点及对分离过程的特殊要求, 掌握生物分离最基本的各种技术，特别是生物大分子的分离、 纯化、制备、检测等，包括各种方法的基本原理、过程、设备、计算和应用，了解生物分离 技术的发展趋势，培养学生结合基础知识分析解决试验研究和工业化生产可能遇到的根本问 题的能力，为进一步学习专业课以及参加专业设计与科研打下基础。 三、教学方法与手段 课堂采用多媒体教学，课件撰写语言为英语，课堂讲授以中英文交替进行，重点难点内 容以中文讲授，一般内容以及结论性内容以英语讲解。 四、教学内容及要求 Chapter 1 Introduction In this chapter, students should grasp concepts of bioseparation and bioproducts, four stages in bioseparation, and four parameters to analyze bioseparation efficiency. They should get familiar with the diversity of bioproducts and the basis for bioseparation. Furthermore, they should understand the historical development of bioseparation techniques, the importance of bioseparation for the production of various bioproducts. Key points：Concepts of bioseparation and bioproducts. Basis for biosepara tion. Difficult points：Engineering analysis of bioseparation. Chapter 2 Cell disruption In this chapter, students should grasp the principles, operations, applications http://spxy.zjgsu.edu.cn and limitations of the common used cell disruption techniques—including mechanic and non-mechanic ones, get familiar with the structural differences among different living cells, understand why it is necessary to disrupt the cells for production of some bioproducts, how to select an appropriate disruption technique, how to evaluate the effectiveness of an disruption method, and why we should keep an appropriate extent of cell disruption. Key points：Principles, operations, applications and limitations of cell disruption techniques. Difficult points：Principles of cell disruption methods, osmotic pressure. Chapter 3 Crystallization and precipitation In this chapter, students should grasp solubility and supersolubility diagram, ways to generate supersaturated solutions, and nucleation phenomena, get familiar with the similarities and differences between crystallization and precipitation, and understand the common crystallization paths, and the importance of different nucleation processes to industrial application. Key points：solubility and supersolubility diagram, nucleation phenomena, definition of crystallization and precipitation. Difficult points：nucleation rate, crystal growth rate. Chapter 4 Membrane Filtration In this chapter, students should grasp membrane selectivity, driving forces for membrane filtration, and membrane filtration methods of microfiltration and ultrafiltration, get familiar with reverse osmosis and electrodialysis, understand membrane structures, membrane modules, membrane fouling, and the general application of membrane filtration. Key points：selectivity of membrane filtration, structural diversity in membranes, Darcy’s law, concentration polarization. Difficult points： Darcy’s law, concentration polarization, solvent and solute flux in membrane filtration. Chapter 5 Centrifugation In this chapter, students should grasp gravitational and centrifugal sedimentation, calculations about tubular bowl centrifuges and disk-type centrifuges, get familiar with the two methods for industrial scale-up of centrifugation, namely Σ factor and equivalent time, understand the commonly used http://spxy.zjgsu.edu.cn centrifugation techniques, including differential centrifugation and density gradient centrifugation. Key points：Calculations of terminal and rotational velocity, the ways to describe a tubular bowl centrifugation and a disk-type centrifugation. Difficult points：Movement trajectory of particle in the centrifuge. Chapter 6 Chromatography In this chapter, students should grasp the four basic elements of a chromatographic system, the types of mobile phases and stationary phases, the detection methods, the chromatogram and the related information, get familiar with the principles, features, compositions, applications of the widely used chromatographic techniques, including gel-permeation chromatography, ion-exchange chromatography, affinity chromatography, understand the ways to analyze chromatographic processes. Key points：Knowing about the chromatogram, the principles and calculations about various chromatographic techniques. Difficult points：Ways to analyze chromatographic processes. Chapter 7 Extraction In this chapter, students should grasp the chemical thermodynamics of partitioning, several widely used extraction techniques, including organic-aqueous extraction, two-phase aqueous extraction, reverse micelle extraction, and supercritical fluid extraction. They should get familiar with the configurations for stage-wise contacting, including cocurrent, crosscurrent and countercurrent contacting. Furthermore, they should understand the features and applications of the different techniques. Key points：Chemical thermodynamics of partitioning and several widely use d extraction techniques. Difficult points：Configurations for stage-wise contacting. Chapter 8 Electrophoresis In this chapter, students should grasp the basic principle of electrophoresis, including the electrophoretic mobility and the influential factors. They should get familiar with the principles and operations of the widely used electrophoretic techniques, including zone electrophoresis, capillary electrophoresis, isoelectric focusing, and two-dimensional electrophoresis. Moreover, they will have an idea of the historical development and the specific applications of these electrophoretic http://spxy.zjgsu.edu.cn techniques. Key points：Electrophoretic mobility and the influential factors. Principl es and operations of the widely used electrophoretic techniques. Difficult points：Electrophoretic mobility and electroosmosis. Chapter 9 Solvent Removal and Drying In this chapter, students should grasp the differentiation between evaporation and drying, the free water and bound water in biological solids, the stages during a drying process. They should get familiar with the principles of spray drying and freeze drying and understand other equipment for evaporation and drying, including flash evaporators, fluidized bed dryers, flash drying, etc. Key points：Definition of evaporation and drying. Free water and bound wat er. Stages during a drying process. Operating principles of spray drying and freeze drying. Difficult points：Stages during a drying process. Chapter 10 Bioprocess Design and Economics In this chapter, students should have a better grasp of the general diagram of downstream processing, the bioseparation process heuristics, get familiar with product formulation, including encapsulation. Key points：General diagram of downstream processing. Difficult points ：Choice of an appropriate technique in the downstream processing. Chapter 11 Trends in Bioseparation Through presentation and discussion, the students will have an understanding of what progresses have been or are being made in bioseparation techniques for bioproducts, and be inspired to work in this field. 五、教学时数分配表 章 内 容 总参考时数 1 Introduction 2 2 Cell Disruption 2 3 Crystallization and precipitation 3 4 Membrane Filtration 4 5 Centrifugation 3 6 Chromatography 4 http://spxy.zjgsu.edu.cn 7 Extraction 4 8 Electrophoresis 4 9 Solvent Removal and Drying 2 10 Bioprocess Design and Economics 2 11 Trends in Bioseparation & Review 2 合 计 32 六、考核方式及成绩评定标准 考核方式：闭卷 成绩评定标准：平时成绩在总成绩中占 50%，期末考试成绩在总成绩中占 50%，成绩评 定为百分制，总成绩=平时成绩×50%+期末成绩×50%。平时成绩包括期中考试、课堂讨论、 测验、作业、出勤情况等。 七、教材及主要参考书 教材：Antonio A. García 等，Bioseparation process sc ience 生物分离工程科学， 北京：清华大学出版社，第 1 版，2002 年 5 月 参考书目： [1]、[美]Antonio A. García 等著，刘铮、詹劲等译，生物分离过程科学，北京：清 华大学出版社，第一版，2004 年 5 月 [2]、PA Belter, EL Cussler and WS Hu，Bioseparations: Downstream Processing for Biotechnology，John Wiley and Sons，1 st ed，1988 [3]、JD Seader and EJ Henley，Separation Process Principles，John Wiley and Sons， 1997 [4]、严希康，生化分离工程，北京：化学工业出版社，第 1 版，2001 年 2 月 执笔人: 石锦芹